@article{Bueno_Biasi_Tofanelli_2018, title={Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)}, volume={12}, url={https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7226}, DOI={10.17584/rcch.2018v12i2.7226}, abstractNote={<p>This study presents the first micropropagation protocol for greenberry (<em>Rubus erythroclados</em>), a wild Brazilian species with edible green fruits. In the <em>in vitro </em>multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, <em>in </em>and <em>ex vitro </em>rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the <em>in vitro </em>trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the <em>ex vitro </em>trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. <em>R. erythroclados </em>multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The <em>ex vitro </em>rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for <em>R. erythroclados </em>micropropagation with 5 μM BA for <em>in vitro </em>shoot proliferation and <em>ex vitro </em>rooting of microcuttings with intermittent misting.</p>}, number={2}, journal={Revista Colombiana de Ciencias Hortícolas}, author={Bueno, Paulo Mauricio Centenaro and Biasi, Luiz Antonio and Tofanelli, Mauro Brasil Dias}, year={2018}, month={May}, pages={405–415} }