Effects of indolebutyric acid, stem cutting positions and substrates on the vegetative propagation of <i>Stevia rebaudiana</i> Bertoni

Authors

DOI:

https://doi.org/10.17584/rcch.2018v12i1.6631

Keywords:

Auxin, cuttings, natural sweetener, seedling production.

Abstract

Stevia rebaudiana Bertoni is a species with high economic importance because of the production of natural sweeteners with a low caloric value. The seed production of this species is irregular and micropropagation techniques raise the cost of seedling production. Thus, this study aimed to optimize the vegetative propagation of a Brazilian accession of this species through stem cuttings. The plant material was collected from one-year-old mature plants. Two experiments were carried out to determine the best indolebutyric acid (IBA) concentration to improve rooting and to evaluate the interaction between the stem cutting position (apical, medium and basal) and substrates (Tropstrato HT®, vermiculite, and vermiculite/Tropstrato HT® mixture). The rooting of the stem cuttings was affected by the IBA concentrations. The application of 710 mg L-1 was recommended for higher rooting percentages. The stem cuttings from the apical branch region had higher rooting percentages than the medium and basal areas (means of 70, 45.8 and 7.5%, respectively). Similar results were observed for the root numbers, root lengths and root fresh and dry mass. The substrate Tropstrato HT® promoted higher rooting percentages and lower mortality than vermiculite.

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Author Biography

Erik Nunes Gomes, Universidade Federal do Paraná, Departamento de Fitotecnia e Fitossanitarismo, Setor de Ciências Agrárias, Curitiba-PR

Licenciado em Ciêncas Agrícolas - Instituto Federal Catarinense;

Mestrado em Agronomia/Produção Vegetal - Universidade Federal do Paraná.

Doutorado em Andamento em Agronomia/Produção Vegetal - Universidade Federal do Paraná.

References

Abdullateef, R.A. and M. Osman. 2012. Effects of stem cutting types, position and hormonal factors on rooting in Stevia rebaudiana Bertoni. J. Agr. Sci. 4(1), 49-57. Doi: 10.5539/jas.v4n1p49

Alcantara, G.B., Y. Oliveira, D.M. Lima, L.A. Fogaça, F. Pinto, and L.A. Biasi. 2010. Efeito dos ácidos naftaleno acético e indolilbutírico no enraizamento de estacas de jambolão [Syzygium cumini (L.) Skeels]. Rev. Bras. Plantas Med. 12(3), 317-321. Doi: 10.1590/S1516-05722010000300009

Amaro, H.T.R, J.R. Silveira, A.M.S.S. David, M.A.V. Resende, and J.A.S. Andrade. 2013. Tipos de estacas e substratos na propagação vegetativa da menta (Mentha arvensis L.). Revista Rev. Bras. Plantas Med. 15(3), 313-318. Doi: 10.1590/S1516-05722013000300001

Bischoff, A., D. Vendramim, E. Nunes Gomes, K.C. Zuffellato-Ribas, M.L. Engel, and R.A. Maggioni. 2017. Enraizamento de estacas de erva-baleeira em função de diferentes concentrações de ácido indol butírico e número de folhas. Rev. Ciênc. Agroveterinárias 16(1), 41-47. Doi: 10.5965/223811711612017041

Bona, C.M., I.R. Biasetto, M. Masetto, C. Deschamps, and L.A. Biasi. 2012. Influence of cutting type and size on rooting of Lavandula dentata L. Rev. Bras. Plantas Med. 14(1), 8-11. Doi: 10.1590/S1516-05722012000100002

Bona, C.M., L.A., F. Zanette, and T. Nakashima. 2005. Estaquia de três espécies de Baccharis. Cienc. Rural. 35 (1), 223-226. Doi: /10.1590/S0103-84782005000100037

Brasil (Ministério do Desenvolvimento, Indústria e Comércio Exterior). Secretaria do Comércio Exterior. 2014. Programa AliceWeb. 2014. In: http://aliceweb.desenvolvimento.gov.br; consulted: May 26, 2014.

Chatsudthipong, V. and C. Muanprasat. 2009. Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacol. Ther. 121(1), 41–54. Doi: 10.1016/j.pharmthera.2008.09.007

Cunha, A., F. Chaves, A. Batista, and A. Hidalgo. 2015. Propagação vegetativa de estacas de Piper hispidum Sw. em diferentes substratos. Rev. Bras. Plantas Med. 17(4), 685-692. Doi: 10.1590/1983-084X/14_113

De Carvalho, M.A.M. and L.B.P. Zaidan. 1995. Obtenção de estacas de Stevia rebaudiana através de estacas. Pesq. Agropec. Bras. 30(2), 201-206.

Fachinello, J. C., A. Hoffmann, J. C. Nachtigal, E. Kersten, and G.R.L. Fortes. 1995. Propagação de plantas frutíferas de clima temperado. 2nd ed. Editora UFPEL, Pelotas- RS, Brazil.

Garbuio, C., L.A. Biasi, A. Kowalski, D. Signor, E. Machado, and C. Deschamps. 2007. Propagação por estaquia em patchouli com diferentes números de folhas e tipos de estacas. Sci. Agr. 8(4), 435-438. Doi: 10.5380/rsa.v8i4.9893

Goettemoeller, J. and A. Ching. 1999. Seed germination in Stevia rebaudiana. pp. 510-511. In: Janick, J. (ed.). Perspectives on new crops and new uses. ASHS Press, Alexandria, VA, USA.

Hartmann, H., D. Kester, F. Davies, and R. Geneve. 2010. Plant propagation. Principles and practices. 8th ed. Prentice Hall, New Jersey, NJ, USA.

Jarma, A., M. Espitia, and G. Fischer. 2010. Síntesis de esteviósidos en estevia (Stevia rebaudiana Bert.). Acta Biol. Colomb. 15(1), 263-268.

Kassahun, B. M. and S.A. Mekonnen. 2011. Effect of cutting position and rooting hormone on propagation ability of stevia (Stevia rebaudiana Bertoni). J. Plant. Biochem. Biotechnol. 6(1), 5-8.

Khalil, S.A., R. Zamir, and N. Ahmad. 2014. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni). Saudi. J. Biol. Sci. 21(6), 566-573. Doi: 10.1016/j.sjbs.2014.02.005

Koppad, A.G., N.K. Umarbadshah, and M. Gouda. 2006. Effect of growth regulators on propagation of stem cuttings in Stevia rebaudiana. Karnataka J. Agric. Sci. 19(3), 740-742.

LeCroy, J. 2014. Factors affecting seed and stem cutting propagation of Stevia rebaudiana (Bert.). M.Sc. thesis. Graduate Faculty, Auburn University, Auburn, AL, USA.

Lemus-Mondaca, R., A.Vega-Gálvez, L. Zura-Bravo, and K. Ah-Hen. 2012. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem. 132(3), 1121-1132. Doi: 10.1016/j.foodchem.2011.11.140

Lima Filho, O.F., A.C. Valois, and Z.M. Lucas. 2004. Sistemas de Produção 5: Estévia. Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Agropecuária Oeste, Dourados-MS, Brazil.

Magevski, G.C., M.P. Czepak, E.R. Schmildt, R.S. Alexandre, and A.A. Fernandes. 2011. Propagação vegetativa de espécies silvestres do gênero Piper, com potencial para uso como porta enxertos em pimenta-do-reino (Piper nigrun). Rev. Bras. Plantas Med. 13(special), 559-563. Doi: 10.1590/S1516-05722011000500009

Mandal, S., H. Evelina, B.B. Giri, V. P.Singha, and R. Kapoor. 2013. Arbuscular mycorrhiza enhances the production of stevioside and rebaudioside-A in Stevia rebaudiana via nutritional and non-nutritional mechanisms. Appl. Soil Ecol. 72(1), 187-194. Doi: 10.1016/j.apsoil.2013.07.003

Medina, E.L., A.E.G. Rivero, and A.L. Zavaleta. 2016. Enraizamiento de esquejes de Stevia rebaudiana Bertoni (Asteraceae) “estevia”, aplicando dosis creciente de ácido indolbutírico. Arnaldoa, 23(2), 569-576. Doi: 10.22497/arnaldoa.232.23209

Mendes, A.D.R., T.H.S. Lacerda, S.M.G. Rocha, and E.R. Martins. 2014. Reguladores vegetais e substratos no enraizamento de estacas de erva-baleeira (Varronia curassavica Jacq.). Rev. Bras. Plantas Med. 16(2), 262- 270. Doi: 10.1590/S1516-05722014000200015

Nunes Gomes, E. and D. Krinski. 2016. Propagação vegetativa de Piper amalago L. (Piperaceae) em função de tipos de estaca e substratos. Cult. Agron. 25(3), 199-210.

Osman, M., N.S. Samsudin, G. Faruq, and A. Nezhadahmadi. 2013. Factors affecting microcuttings of Stevia using a mist-chamber propagation box. Sci. World J. 2013, 1-10. Doi: 10.1155/2013/940201

Patel, D.K. 2015. Stem cutting propagation for ex-situ conservation of Stevia rebaudiana (Bertoni) Bertoni in herbal garden. Eur. J. Biotechnol. 3(9), 35-42.

Paulus, D., R. Valmorbida, E. Toffoli, and E. Paulus. 2014. Propagação vegetativa de Aloysia triphylla (L’Hér.) Britton em função da concentração de AIB e do comprimento das estacas. Rev. Bras. Plantas Med.16(1), 25-31. Doi: 10.1590/S1516-05722014000100004

Ramesh, K., V. Singh, and P.S. Ahuja. 2007. Production potential of Stevia rebaudiana (Bert.) Bertoni. under intercropping systems. Arch. Agron. Soil Sci. 53(4), 443-458. Doi: 10.1080/03650340701474949

Saad, A., F.A. Khan, A. Hayee, and M.S. Nazir. 2014. A Review on Potential Toxicity of Artificial Sweetners vs Safety of Stevia: A Natural Bio-Sweetner. J. Biol. Agric. Healthc. 4(15) 137-145.

Silva, F.A.S. and C.A.V. Azevedo. 2016. Comparison of means of agricultural experimentation data through different tests using the software Assistat. Afr. J. Agric. Res. 37(11), 3527-3531. Doi: 10.5897/AJAR2016.11523

Sivaram, L. and U. Mukundam. 2003. In vitro culture studies on Stevia rebaudiana. In Vitro Cell. Dev. Biol. 39(5), 520–523. Doi: 10.1079/IVP2003438

Smitha, G.R. and K. Umesha. 2012. Vegetative propagation of stevia [Stevia rebaudiana (Bertoni) Hems.] through cuttings. J. Trop. Agr. 50(1), 72-75.

Tracz, V., C.T.A. Cruz-Silva, and M.Z. Luz. 2014. Produção de mudas de penicilina (Alternanthera brasiliana (L.) Kuntze) via estaquia. Rev. Bras. Plantas Med. 16(3), 644-648. Doi: 10.1590/1983-084x/12_098

Yadav, A. K., S. Singh, D. Dhyani, and P.S. Ahuja. 2011. A review on the improvement of stevia [Stevia rebaudiana (Bertoni)]. Can. J. Plant Sci., 91(1), 1-27. Doi: 10.4141/cjps10086

Zuffellato-Ribas, K.C. and J.D. Rodrigues. 2001. Estaquia: uma abordagem dos principais aspectos fisiologicos. Editora UFPR, Curitiba-PR, Brazil.

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Published

2018-05-02

How to Cite

Pigatto, G. B., Gomes, E. N., Tomasi, J. de C., Ferriani, A. P., & Deschamps, C. (2018). Effects of indolebutyric acid, stem cutting positions and substrates on the vegetative propagation of <i>Stevia rebaudiana</i> Bertoni. Revista Colombiana De Ciencias Hortícolas, 12(1), 202–211. https://doi.org/10.17584/rcch.2018v12i1.6631

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Section on aromatic, medicinal and spice plants

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