Effect of 6-BAP application on leaf yellowing of Chrysanthemum morifolium Ram cv. 'Shrek' and 'Bomber Green'

Abstract
Growth regulators are used in commercial production of chrysanthemums without certainty about their effects on quality characteristics, particularly sensitivity to yellowing and leaf color loss. Cytokinin (CK) may contribute to the prevention and delay of chlorophyll degradation. This study aimed to quantify the response to the application of 6-benzylaminopurine (6-BAP) on delaying foliage yellowing associated with chlorophyll loss in the chrysanthemum cultivars ‘Shrek’ and ‘Bomber Green.’ 6-BAP was applied at concentrations of 33.3, 66.6, and 100 mg kg-1, alongside a control without CK and a commercial control (TC) containing CK from an algae concentrate. Applications were made at 35, 43, 51, 59, 67, and 75 days after transplanting (DAT). The relative chlorophyll content was quantified using a SPAD-502 meter in leaves from the lower, middle, and upper strata. Additionally, the longevity of the foliage from harvested stems was evaluated under simulated travel conditions and subsequent exposure in vases. The chlorophyll content differed significantly (P<0.05) between cultivars and strata, increasing in the middle and upper strata while decreasing in the lower strata. Vase life was shorter in ‘Shrek,’ at 7 d, with a foliage yellowing severity of grade 1, the onset of which occurred between 5 and 7 days with the use of 66.6 and 100 mg kg-1 of 6-BAP. Moreover, ‘Shrek’ plants exhibited yellowing of the foliage during growth; this physiological issue may be directly related to the plant material and its interaction with the environmental conditions of the cultivation area.
Keywords
Growth regulator, Asteraceae, SPAD, Chlorophylls, Cut flower, Vase life
References
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