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Vol. 26 Núm. 45 (2017)

Articulos

Comparación in vitro de la citocompatibilidad entre los biomateriales fibroína y polipropileno

https://doi.org/10.19053/01211129.v26.n45.2017.6056

Publicado 2017-05-02

  • Alejandro Arboleda-Carvajal
    • ,
  • Julián González
    • ,
  • Manuel Hernando Franco-Arias
    • ,
  • Liliana Valladares-Torres

Alejandro Arboleda-Carvajal
Universidad Icesi (Cali-Valle del Cauca, Colombia).

Julián González
Universidad Icesi (Cali-Valle del Cauca, Colombia).

Manuel Hernando Franco-Arias
Universidad Icesi (Cali-Valle del Cauca, Colombia).

Liliana Valladares-Torres
Universidad Icesi (Cali-Valle del Cauca, Colombia).

Resumen

Este artículo evalúa el crecimiento de células Hela in vitro en presencia de fibroína y polipropileno. Con el objetivo de determinar la proliferación celular en presencia de estos dos biomateriales, se obtuvo el número de células/muestra por medio de la prueba de reducción metabólica del bromuro de 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol (MTT), utilizando evidencia de citotoxicidad directa e indirecta. Las pruebas directas e indirectas de citotoxicidad de fibroína y polipropileno mostraron una diferencia estadísticamente significativa en el promedio de células vivas para fibroína (p<0.005), sin importar el tipo de test. Los métodos in vitro utilizados en este estudio permitieron observar que la fibroína tiene un mejor comportamiento celular, en términos de viabilidad, comparada con el polipropileno.

Palabras clave

Biomateriales, Crecimiento celular, Fibroína, Polímeros, Polipropileno, Seda

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Citas

  1. N. Sawatjui, T. Damrongrungruang, W. Leeanansaksiri, P. Jearanaikoon, and T. Limpaiboon, “Fabrication and characterization of silk fibroin–gelatin/chondroitin ulfate/hyaluronic acid scaffold for biomedical applications,” Materials Letters, vol. 126 (1), pp. 207-210, Jul. 2014. DOI: http://doi.org/10.1016/j.matlet.2014.04.018. DOI: https://doi.org/10.1016/j.matlet.2014.04.018
  2. G. Lai, K. Shalumon, S. Chen, and J. Chen, “Composite chitosan/silk fibroin nanofibers for modulation of osteogenic differentiation and proliferation of human mesenchymal stem cells,” Carbohydr Polym, vol. 111 (1), pp. 288-97, Oct. 2014. DOI: http://doi.org/10.1016/j.carbpol.2014.04.094. DOI: https://doi.org/10.1016/j.carbpol.2014.04.094
  3. F. G. Omenetto, and D. L. Kaplan, “New Opportunities for an Ancient Material,” Science, vol. 329 (5991), pp. 528-531, Jul. 2010. DOI: http://doi.org/10.1126/science.1188936. DOI: https://doi.org/10.1126/science.1188936
  4. Y. Cao, and B. Wang, “Biodegradation of Silk Biomaterials,” Int J Mol Sci, vol. 10 (4), pp. 1514-1524, Mar. 2009. DOI: http://doi.org/10.3390/ijms10041514. DOI: https://doi.org/10.3390/ijms10041514
  5. H. Jin, J. Park, R. Valluzi, P. Cebe, and D. Kaplan, “Biomaterial films of Bombyx mori silk fibroin with poly(ethylene oxide),” Biomacromolecules, vol. 5 (3), pp. 711-717, May. 2004. DOI: http://doi.org/10.1021/bm0343287. DOI: https://doi.org/10.1021/bm0343287
  6. S. Hofmann, C. Foo, F. Rossetti, M. Textor, N. Vunjak, D. Kaplan, H. Merkle, and. Meinel, “Silk fibroin as an organic polymer for controlled drug delivery,” J Control Release, vol. 111 (1-2), pp. 219-227, Mar. 2006. DOI: http://doi.org/10.1016/j.jconrel.2005.12.009. DOI: https://doi.org/10.1016/j.jconrel.2005.12.009
  7. U. Kim, J. Park, H. Kim, M. Wada, and D. Kaplan, “Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin,” Biomaterials, vol. 26 (15), pp. 2775-85, May. 2005. DOI: http://doi.org/10.1016/j.biomaterials.2004.07.044. DOI: https://doi.org/10.1016/j.biomaterials.2004.07.044
  8. R. Unger, A. Sartoris, K. Peters, A. Motta, C. Migliare, M. Kunkel, U. Bulnheim, J. Rychly, and C. Kirkpatrick, “Tissue-like self-assembly in cocultures ofendothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials,” Biomaterials, vol. 28 (27), pp. 3965-3976, Sep. 2007. DOI: http://doi.org/10.1016/j.biomaterials.2007.05.032. DOI: https://doi.org/10.1016/j.biomaterials.2007.05.032
  9. Y. Yang, X. Chen, F. Ding, P. Zhang, J. Liu, and X. Gu, “Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro,” Biomaterials, vol. 28 (9), pp. 1643-1652, Mar. 2007. DOI: http://doi.org/10.1016/j.biomaterials.2006.12.004. DOI: https://doi.org/10.1016/j.biomaterials.2006.12.004
  10. H. Kim, L. Che, Y. Ha, and W. Ryu, “Mechanically-reinforced electrospun composite silk fibroin nanofibers containing hydroxyapatite nanoparticles,” Mater Sci Eng C Mater Biol Appl., vol. 40 (1), pp. 324-35, Jul. 2014. DOI: http://doi.org/10.1016/j.msec.2014.04.012. DOI: https://doi.org/10.1016/j.msec.2014.04.012
  11. H. Kim, U. Kim, H. Kim, C. Li, M. Wada, G. Leisk, and D. Kaplan, “Bone tissue engineering with premineralized silk scaffolds,” Bone, vol. 42 (6), pp. 1226-1234, Jun. 2008. DOI: http://doi.org/10.1016/j.bone.2008.02.007. DOI: https://doi.org/10.1016/j.bone.2008.02.007
  12. S. Sahoo, S. Toh, and J. Goh , “A bFGF-releasing silk/PLGA-based biohybrid scaffold for ligament/tendon tissue engineering using mesenchymal progenitor cells,” Biomaterials, vol. 31 (11), pp. 2990-2998, Apr. 2010. DOI: http://doi.org/10.1016/j.biomaterials.2010.01.004. DOI: https://doi.org/10.1016/j.biomaterials.2010.01.004
  13. M. Wolf, C. Dearth, C. Ranallo, S. LoPresti, L. Carey, K. Daly, B. Brown, and S. Badylak, “Macrophage polarization in response to ECM coated polypropylene mesh,” Biomaterials, vol. 35 (25), pp. 6838-6849, Aug. 2014. DOI: http://doi.org/10.1016/j.biomaterials.2014.04.115. DOI: https://doi.org/10.1016/j.biomaterials.2014.04.115
  14. W. Cobb, K. Kercher, and B. Heniford, “The argument for lightweight polypropylene mesh in hernia repair,” Surg Innov, vol. 12 (1), pp. 63-69, Mar. 2005. DOI: http://doi.org/10.1177/155335060501200109. DOI: https://doi.org/10.1177/155335060501200109
  15. A. McNally, and J. Anderson, “Macrophage fusion and multinucleated giant cells of inflammation,” Adv Exp Med Biol, vol. 713 (1), pp. 97-111, 2011. DOI: http://doi.org/10.1007/978-94-007-0763-4_7. DOI: https://doi.org/10.1007/978-94-007-0763-4_7
  16. J. M. Anderson, A. Rodriguez, and D. T. Chang, “Foreign body reaction to biomaterials,” Semin Immunol, vol. 20 (2), pp. 86-10, Apr. 2008. DOI: http://doi.org/10.1016/j.smim.2007.11.004. DOI: https://doi.org/10.1016/j.smim.2007.11.004
  17. D. Rockwood, R. Preda, T. Yücel, X. Wang, M. Lovett, and D. Kaplan , “Materials fabrication from Bombyx mori silk fibroin,” Nat Protoc, vol. 6 (10), pp. 1612-1631, Sep. 2011. DOI: http://doi.org/10.1038/nprot.2011.379. DOI: https://doi.org/10.1038/nprot.2011.379
  18. S. Farè, P. Torricelli, G. Giavaresi, S. Bertoldi, A. Alessandrino, T. Villa, M. Fini, M. C. Tanzi, and G. Freddi, “In vitro study on silk fibroin textile structure for Anterior Cruciate Ligament regeneration,” Materials Science and Engineering: C, vol. 33 (7), pp. 3601-3608, Oct. 2013. DOI: http://doi.org/10.1016/j.msec.2013.04.027. DOI: https://doi.org/10.1016/j.msec.2013.04.027
  19. P. Amornsudthiwat, R. Mongkolnavin, S. Kanokpanont, J. Panpranot, C. Wong, and S. Damrongsakkul, “Improvement of early cell adhesion on Thai silk fibroin surface by low energy plasma,” Colloids Surf B Biointerfaces, vol. 111 (1), pp. 579-586, Nov. 2013. DOI: http://doi.org/10.1016/j.colsurfb.2013.07.009. DOI: https://doi.org/10.1016/j.colsurfb.2013.07.009
  20. G. Sternschuss, D. Ostergard, and H. Patel, “Post-implantation alterations of polypropylene in the human,” J Urol, vol. 188 (1), pp. 27-32, Jul. 2012. DOI: http://doi.org/10.1016/j.juro.2012.02.2559. DOI: https://doi.org/10.1016/j.juro.2012.02.2559
  21. D. Zhang, Z. Y. Lin, R. Cheng, W. Wu, J. Yu, X. Zhao, X. Chen, and W. Cui, “Reinforcement of transvaginal repair using polypropylene mesh functionalized with basic fibroblast growth factor,” Colloids and Surfaces B: Biointerfaces, vol. 142 (1), pp. 10-19, 2016. DOI: http://doi.org/10.1016/j.colsurfb.2016.02.034. DOI: https://doi.org/10.1016/j.colsurfb.2016.02.034
  22. B. Brown, D. Mani, A. Nolfi, R. Liang, S. Abramowitch, and P. Moalli, “Characterization of the host inflammatory response following implantation of prolapse mesh in rhesus macaque,” American Journal of Obstetrics and Gynecology, vol. 213 (15), pp. 668.e1–668.e10, 2015. DOI: http://doi.org/10.1016/j.ajog.2015.08.002. DOI: https://doi.org/10.1016/j.ajog.2015.08.002
  23. I. Hejazi, J. Seyfi, E. Hejazi, G. Sadeghi, S. Jafar, and H. Khonakdar, “Investigating the role of surface micro/nano structure in cell adhesion behavior of superhydrophobic polypropylene/nanosilica surfaces,” Investigating the role of surface micro/nano structure in cell adhesion behavior of superhydrophobic polypropylene/nanosilica surfaces, vol. 127 (1), pp. 233-240, 2015. DOI: https://doi.org/10.1016/j.colsurfb.2015.01.054
  24. Y. Maghdouri-White, G. Bowlin, C. Lemmon, and D. Dréau, “Mammary epithelial cell adhesion, viability, and infiltration on blended or coated silk fibroin–collagen type I electrospun scaffolds,” Materials Science and Engineering: C, vol. 43 (1), pp. 37-44, Oct. 2014. DOI: http://doi.org/10.1016/j.msec.2014.06.037. DOI: https://doi.org/10.1016/j.msec.2014.06.037
  25. H. Sakagami, M. Satoh, Y. Yokote, H. Takano, M. Takahama, M. Kochi, and K. Akahane, “Amino acid utilization during cell growth and apoptosis induction,” Anticancer Res, vol. 18 (6), pp. 4303-4306, Nov. 1998.
  26. S. Sofia, M. McCarthy , G. Gronowicz, and D. Kaplan , “Functionalized silk-based biomaterials for bone formation,” Journal of biomedical materials research, vol. 54 (1), pp. 139-148, 2001. DOI: http://doi.org/10.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-7.
  27. L. Jia, L. Guo, J. Zhu, and Y. Ma, “Stability and cytocompatibility of silk fibroin-capped gold nanoparticles,” Mater Sci Eng C Mater Biol Appl, vol. 43 (1), pp. 231-236, Oct. 2014. DOI: http://doi.org/10.1016/j.msec.2014.07.024. DOI: https://doi.org/10.1016/j.msec.2014.07.024
  28. W. Cui, J. Li, Y. Zhang, H. Rong, W. Lu, and L. Jiang, “Effects of aggregation and the surface properties of gold nanoparticles on cytotoxicity and cell growth,” Nanomedicine, vol. 8 (1), pp. 46-53, Jan. 2012. DOI: http://doi.org/10.1016/j.nano.2011.05.005. DOI: https://doi.org/10.1016/j.nano.2011.05.005
  29. C. Zhang, J. Jin, J. Zhao, W. Jiang, and J. Yin, “Functionalized polypropylene non-woven fabric membrane with bovine serum albumin and its hemocompatibility enhancement,” Colloids Surf B Biointerfaces, vol. 102 (1), pp. 45-52, Feb. 2013. DOI: http://doi.org/10.1016/j.colsurfb.2012.08.007. DOI: https://doi.org/10.1016/j.colsurfb.2012.08.007
  30. A. Prudente, C. L. Riccetto, M. M. Simões, B. M. Pires, and M. G. de Oliveira, “Impregnation of implantable polypropylene mesh with S-nitrosoglutathione-loaded poly(vinyl alcohol),” Colloids Surf B Biointerfaces, vol. 108 (1), pp. 178-184, Aug. 2013. DOI: http://doi.org/10.1016/j.colsurfb.2013.02.018. DOI: https://doi.org/10.1016/j.colsurfb.2013.02.018
  31. N. Gomathi, R. Rajasekar, R. Rajesh Babu, D. Mishra, and S. Neogi, “Development of bio/blood compatible polypropylene through low pressure nitrogen plasma surface modification,” Materials Science and Engineering: C, vol. 32 (7), pp. 1767-1778, Oct. 2012. DOI: http://doi.org/10.1016/j.msec.2012.04.034. DOI: https://doi.org/10.1016/j.msec.2012.04.034

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