Andamios eletrohilados de poli(ɛ-caprolactona) /colágeno con uso potencial en regeneración de tejido cutáneo
Resumen
Dentro de los tratamientos actuales para la reparación de las lesiones en piel se encuentran los autoinjertos, los aloinjertos ylos sustitutos dérmicos biosintéticos. Sin embargo, solo la terapia con autoinjerto es la que da mejores resultados y está sujeta al área de piel afectada en el paciente quemado, los demás tratamientos solo dan cobertura temporal a la herida, evidenciando la necesidad de generar estructuras que además de proteger la herida posean funciones biológicas que contribuyan a los procesos de reparación o regeneración de la piel. Con el fin de generar un apósito que reúna estas características, se fabricaron andamios de poli(ℇ-caprolactona) /Colágeno tipo I por electrohilado como posibles sustitutos dérmicos. Estos andamios se caracterizaron porSEM, ángulo de contacto, ATR-FTIR, TGA, DSC y se determinó su biocompatibilidad a partir de laadhesión y proliferación decélulas estromales mesenquimales de gelatina de Wharton (CEM-GW). Se encontró que la presencia de colágeno en los andamios disminuye el diámetro de fibra y mejora la hidrofilicidad favoreciendo los procesos de adhesión de las CEM-GW. Además, se demostró que no es necesario utilizar altas cantidades de colágeno para obtener un andamio con características fisicoquímicas y biológicas favorables.
Palabras clave
Sustitutos dérmicos, CEM-GW, lesiones cutáneas, biocompatibilidad.
Citas
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