Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes
Abstract
In this work, lanthanide complexes were synthesized starting from the corresponding La (III), Ce (III), Sm (III) and Yb (III) chlorides and cinnamate ligands which present bidentate coordination between the carboxyl group of the ligand and the lanthanide metal. These compounds were used as hosts of β-cyclodextrin to obtain new inclusion complexes by a co-precipitation method using N,N-dimethylformamide as solvent. The inclusion products were characterized by IR-ATR spectroscopy, Raman, UV-vis, 1H and 13C NMR, XRD, TGA-DSC, elemental analysis and EDTA complexometry. Antibacterial activity tests were performed using six ATTC strains (S. aureus ATCC 25923, S. aureus ATCC 29213, E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. Typhimurium ATCC 14028 and K. pneumoniae ATCC BAA-2146) by the microdilution method with Mueller-Hinton broth. The results of the biological activity for the lanthanide complexes showed the synergistic effect between the lanthanide cation and the cinnamate ligand. For the inclusion complexes, a decrease of the minimum inhibitory concentration (MIC) was observed with respect to the initial lanthanide complexes. The results obtained with the bovine thymus DNA and the plasmid pBR322 DNA allow to propose an electrostatic interaction between the evaluated complexes and the molécular structure of the DNA.Keywords
antibacterial activity, inclusion complexes, interaction with DNA, lanthanide complexes
Author Biography
Dorian Polo Cerón
Es Químico por la Universidad Nacional de Colombia (1998-2003), trabajó un año en la industria colombiana desempeñando el cargo de jefe de producción y realizó sus estudios de Doctorado en España (2003-2008) en el Departamento de Tecnología Química y Ambiental (Universidad Rey Juan Carlos) a través de una beca predoctoral, obteniendo el grado de Doctor con Mención de Doctor Europeo con la máxima calificación (Sobresaliente “cum laude”) en septiembre de 2008. Su Tesis Doctoral de título “Síntesis de Nuevos Complejos Metaloceno con Aplicaciones en la Polimerización de Olefinas”. Durante su tesis doctoral, realizó una estancia predoctoral de tres meses, en Inglaterra, como Becario en la Universidad de Bristol, bajo la supervisión del profesor Robin Bedford.
En el año 2009 realizó una estancia postdoctoral (14 meses) en el grupo de los profesores Glen Deacon y Peter Junk en Monash University. Durante este período postdoctoral en Australia, investigó sobre “Síntesis de complejos metálicos con lantánidos, empleando como ligandos formamidinas y carbodimidas”.
A partir de Agosto de 2010, después de participar en un concurso de méritos obtuvo la plaza como profesor nombrado de la Universidad del Valle, en el área de Química Inorgánica. Actualmente, está vinculado al grupo de investigación LICAP categorizado como A por Colciencias, en donde dirige la línea de investigación en Bioinorgánica. Durante su carrera investigadora, ha participado en 9 proyectos de investigación: Unión Europea (1), Universidades (8), los cinco últimos como investigador principal, en la actualidad dos de ellos se encuentran en desarrollo. Ha publicado hasta la fecha un total de 17 artículos en revistas internacionales de reconocido prestigio. Además, ha escrito el libro: “Aplicaciones de complejos metaloceno en la polimerización de olefinas”. Ha presentado un total de 24 Contribuciones a congresos, siete (7) de ellas como comunicaciones orales.
El Dr. Polo-Cerón mantiene colaboraciones con el profesor Peter Junk (Monash University) sobre la síntesis de complejos lantánidos y con el profesor Santiago Gómez Ruiz (Universidad Rey Juan Carlos) sobre la síntesis de complejos metálicos con posible actividad biológica. Igualmente, ha planteado colaboraciones científicas con profesores asociados a la Universidad de Antioquia, Universidad Santiago de Cali y con investigadores del PECET y el Laboratorio de Salud Departamental Aníbal Patiño.
Ha dirigido 3 proyectos fin de carrera para la obtención del título en Ingeniería Química (Universidad Rey Juan Carlos). En el Departamento de Química de la Universidad del Valle ha dirigido tres (3) trabajos de pregrado, una (1) tesis de maestría y actualmente es director de cuatro (4) estudiantes de pregrado y tres (3) estudiantes de postgrado. Es editor asociado de EJournal of Chemistry at Hindawi Publishing Corporation y hace parte del comité del programa del Departamento de Química de la Universidad del Valle.
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