Extraction of Bacterial DNA from Water Sources of Agricultural Use

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Autores

Nicolas Forero Pineda https://orcid.org/0000-0003-2421-5674
Johana Marín - Suárez https://orcid.org/0000-0001-6988-8093
Fabio Emilio Forero- Ulloa https://orcid.org/0000-0001-6815-6579
Andrés Gómez-Palacio https://orcid.org/0000-0002-1069-9199

Abstract

Within the agricultural process different types of water bodies are used, therefore knowledge of crop productivity must include the understanding of the flow of microbiological material present in the used water sources. The last methodological approach aims at the identification of bacterial communities through the analysis of DNA sequences existent in environmental samples. Due to the high contamination that environmental samples may have, it is important to perform an optimal DNA extraction process that allows subsequent molecular analysis by PCR-based methods. For this reason, the objective of this article is to describe a chemical protocol for the extraction of bacterial DNA from water bodies used in local agricultural activities that is simple, efficient, and quick to apply to get good-quality DNA. Bacterial DNA was extracted from water samples obtained from Tota Lake (Boyacá, Colombia) using a modified chemical protocol and compared with a standard commercial method. As a result, bacterial DNA was obtained with a concentration higher than 140 ng/ul and a purity >1.7 A260/280, results which are similar to those obtained with the commercial standard method with a maximum concentration of 45.94 ng/ul and a purity higher than 1.8 A260/280. The results suggest that this DNA extraction protocol is a fast and low-cost method with which high quality and purity DNA is obtained that can be used in any molecular analysis.   

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