Micropropagation and phytopathology of calla lily (Zantedeschia spp.)
Abstract
Calla lily (Zantedeschia spp.) is an herbaceous flowering plant that belongs to the Araceae family, with worldwide distribution. Native to the swampy or mountainous regions of South Africa, it is valued as an ornamental plant due to its extraordinary spathe and decorative leaves. However, its production has decreased due to the presence of diseases. In vitro culture of plant cells and tissues has been successfully applied to precisely diagnose and control diseases to produce disease-free plants. This study analyzes information from in vitro techniques applied to this crop, highlighting phytopathological aspects. It considers the different stages of in vitro culture and the process of obtaining and propagating healthy or pathogen-free plants. In vitro culture has proven to be an effective tool for rapid clonal propagation and multiplication of Zantedeschia spp. Sanitary management before the in vitro culture is recommended.
Keywords
Tissue culture, Embryogenesis, Organogenesis, Totipotency, Pectobacterium
References
- Cafrune, E.E., F. Asinari, C.F. Nome, C.F. Perotto, M.C. Quiroga, and V.C. Conci. 2011. Identificación y caracterización del Dasheen mosaic virus en cala (Zantedeschia aethiopica) en Córdoba, Argentina. Fitopatol. Colomb. 35(1), 63-67.
- Casierra-Posada, F., M.M. Blanke, and J.C. Guerrero-Guío. 2014. Iron tolerance in calla lilies (Zantedeschia aethiopica). Gesunde Pflanzen 66, 63-68. Doi: https://doi.org/10.1007/s10343-014-0316-y
- Chen, L.-R., T.-C. Hsiung, K.-H. Lin, T.-B. Huang, M.-Y. Huang, and A. Wakana. 2017. Supplementary effect of hydrogen peroxide as a pre–disinfectant for sterilizing rhizome bud explants of Zantedeschia aethiopica L. with chlorine dioxide. J. Fac. Agric. Kyushu Univ. 62(1), 81-86. Doi: https://doi.org/10.5109/1799306
- Chen, L.R., P.R. Lin, and C.J. Huang. 2020. First report of Pectobacterium aroidearum causing soft rot disease of white calla lily in Taiwan. Plant Dis. 104(2), 563. Doi: https://doi.org/10.1094/PDIS-07-19-1462-PDN
- Cheng, Z.J., L. Wang, W. Sun, Y. Zhang, C. Zhou, Y.H. Su, W. Li, T.T. Sun, XY. Zhao, X.G. Li, Y. Cheng, Y. Zhao, Q. Xie, and X.S. Zhang. 2013. Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3. Plant Physiol. 161(1), 240-251. Doi: https://doi.org/10.1104/pp.112.203166
- Ciampi, L., J. Nissen, E. Venegas, R. Fuentes, M. Costa, R. Schöbitz, E. Alvarez, and P. Alvarado. 2009. Identification of two species of Fusarium link that cause wilting of colored callas (Zantedeschia aethiopica (L.) Spreng.) cultivated under greenhouse conditions in Chile. Chilean J. Agr. Res. 69(4), 516-525. Doi: https://doi.org/10.4067/S0718-58392009000400006
- Cruz-Castillo, J.G. 2022. ‘Utopia’: a new Zantedeschia aethiopica. HortScience 57(3), 443. Doi: https://doi.org/10.21273/HORTSCI16321-21
- Cruz-Castillo, J.G. and A. Hernández-Montes. 2022. ‘Sublime’, una nueva variedad de alcatraz (Zantedeschia aethiopica (L.) Spreng). Agro-Divulgación 2(4), 57-58.
- Cruz-Castillo, J.G. and P.A. Torres-Lima. 2017. ‘Deja Vu’: a new calla lily (Zantedeschia aethiopica) cultivar. Rev. Chapingo Ser. Hortic. 23(2), 97-101. Doi: https://doi.org/10.5154/r.rchsh.2017.01.005
- Cuellar-Mandujano, A.K., A. Hernández-Montes, and J.G. Cruz-Castillo. 2022. Atributos intangibles en alcatraz blanco (Zantedeschia aethiopica (L) K. Spreng): significados psicológicos y estructura de valores humanos para el consumidor. Nova Sci. 14(29). Doi: https://doi.org/10.21640/ns.v14i29.3129
- Delgado-Paredes, G.E., C. Vásquez-Díaz, B. Esquerre-Ibañez, P. Bazán-Sernaqué, and C. Rojas-Idrogo. 2021. In vitro tissue culture in plants propagation and germplasm conservation of economically important species in Peru. Sci. Agropec. 12(3), 337-349. Doi: https://doi.org/10.17268/sci.agropecu.2021.037
- Duquenne, B., T. Eeckhaut, S. Werbrouck, and J. Van Huylenbroeck. 2006. In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids. Plant Cell Tiss. Organ Cult. 87(3), 329-331. Doi: https://doi.org/10.1007/s11240-006-9161-8
- García-López, F. 2010. Efecto de la cepa bacteriana CAE-01 y fumigación al suelo sobre la pudrición blanda en el cultivo de alcatraz en La Perla, Veracruz. Msc thesis. Universidad Autónoma Chapingo, Chapingo, Mexico.
- Githeng'u, S.K., S. Nyalala, and L. Gaoqiong. 2016. Antibacterial activity of Coptis chinensis extract against Pectobacterium carotovorum subsp. carotovorum. Int. J. Phytopathol. 5(2), 61-66. Doi: https://doi.org/10.33687/phytopath.005.02.1159
- Gong, X., F. Qu, C. You, W. Sun, and M. Wang. 2008. Establishment of callus induction system from leaves in Zantedeschia. J. Yantai Univ. (Nat. Sci. Engi. Ed.). 3, 221-225.
- Grausgruber-Gröger, S., S. Richter, J.M. Salapura, D.K. Jošić, V. Trkulja, and H. Reisenzein. 2016. First report of Dasheen mosaic virus in Zantedeschia in Bosnia and Herzegovina. New Dis. Rep. 33(1), 13-13. Doi: https://doi.org/10.5197/j.2044-0588.2016.033.013
- Guttman, Y., J.R. Joshi, N. Chriker, N. Khadka, M. Kleiman, N. Reznik, Z. Wei, Z. Kerem, and I. Yedidia. 2021. Ecological adaptations influence the susceptibility of plants in the genus Zantedeschia to soft rot Pectobacterium spp. Hort. Res. 8(1), 13. Doi: https://doi.org/10.1038/s41438-020-00446-2
- Han, I.-S. and J.B. Kim. 2019. Establishment of a regeneration system for the production of calla plants (Zantedeschia spp.) via embryogenic callus culture. J. Plant Biotechnol. 46(1), 32-36. Doi: https://doi.org/10.5010/JPB.2019.46.1.032
- Hashemidehkordi, E., S.N. Mortazavi, and P. Azadi. 2021. An efficient in vitro propagation protocol of pot calla lily (Zantedeschia spp cv. Orania and Sunclub) via tuber production. Int. J. Hort. Sci. Technol. 8(4), 343-351.
- He, P., W. Cui, P. He, S. Munir, X. Li, Y. Wu, Y. Li, S. Asad, P. He, and Y. He. 2021. Bacillus amyloliquefaciens subsp. plantarum KC-1 inhibits Zantedeschia hybrida soft rot and promote plant growth. Biol. Control 154, 104500. Doi: https://doi.org/10.1016/j.biocontrol.2020.104500
- He, S., Y. Yang, Z. Li, X. Wang, Y. Guo, and H. Wu. 2020. Comparative analysis of four Zantedeschia chloroplast genomes: expansion and contraction of the IR region, phylogenetic analyses and SSR genetic diversity assessment. PeerJ, 8, e9132. Doi: https://doi.org/10.7717/peerj.9132
- Hernández-Villaseñor, L.A., M.Z. Reyna-Villela, S.M. Aceves-Villarruel, J. Salazar-flores, E.D. Torres-Sánchez, J.H. Torres-Jasso, and D. Rojas-Bravo. 2018. Effect of carbamazepine on the in vitro propagation of Zantedeschia aethiopica. Int. J. Environ. Sci. Toxicol. Res. 6(2), 12-17.
- Hlophe, N.P., M. Moyo, J. Van Staden, and J.F. Finnie. 2015. Micropropagation of Zantedeschia aethiopica (L.) Spreng.: towards its commercial use in the cut flower industry. Propag. Ornam. Plants 15(2), 73-78.
- Jonytienè, V., R. Masienè, N. Burbulis, and A. Blinstrubienè. 2017. Factors affecting Zantedeschia Spreng. dedifferentiation in vitro. Biologija 63(4), 334-340. Doi: https://doi.org/10.6001/biologija.v63i4.3608
- Krejzar, V., J. Mertelík, I. Pánková, K. Kloudová, and V. Kůdela. 2008. Pseudomonas marginalis associated with soft rot of Zantedeschia spp. Plant Protect. Sci. 44(3), 85-90. Doi: https://doi.org/10.17221/16/2008-PPS
- Kulpa, D. 2016. Micropropagation of calla lily (Zantedeschia rehmannii). Folia Hort. 28(2), 181-186. Doi: https://doi.org/10.1515/fhort-2016-0021
- Lee, Y.S. and J.A. Ko. 2005. Effect of plant growth regulators on in vitro micropropagation of colored calla lily (Zantedeschia spp.). Korean J. Plant Res. 18(1), 154-160.
- Liao, M.-T., D.-F. Mou, Y.-C. Chang, and C.-W. Tsai. 2020. Vector transmission of konjac mosaic virus to calla lily (Zantedeschia spp.) by aphids. Ann. App. Biol. 177(3), 367-373. Doi: https://doi.org/10.1111/aab.12628
- Lim, J.-A., S. Jee, D.H. Lee, E. Roh, K. Jung, C. Oh, and S. Heu. 2013. Biocontrol of Pectobacterium carotovorum subsp. carotovorum using bacteriophage PP1. J. Microbiol. Biotechnol. 23(8), 1147-1153. Doi: https://doi.org/10.4014/jmb.1304.04001
- Martínez-Hernández, A. 2022. Propagación in vitro de nuevas variedades de alcatraz (Zantedeschia aethiopica L. Spreng). MSc thesis. Universidad Autónoma Chapingo, Chapingo, Mexico.
- Mikiciński, A., P. Sobiczewski, M. Sulikowska, J. Puławska, and J. Treder. 2010. Pectolytic bacteria associated with soft rot of calla lily (Zantedeschia spp.) tubers. J. Phytopathol. 158(4), 201-209. Doi: https://doi.org/10.1111/j.1439-0434.2009.01597.x
- Muñoz-Escobar, E.M. and J.A. Palacio-Baena. 2010. Efectos del cloruro de mercurio (HgCl2) sobre la sobrevivencia y crecimiento de renacuajos de Dendrosophus bogerti. Actu. Biol. 32(93), 189-197. Doi: https://doi.org/10.17533/udea.acbi.13814
- Naor, V., J. Kigel, and M. Ziv. 2004a. Hormonal control of inflorescence development in plantlets of calla lily (Zantedeschia spp.) grown in vitro. Plant Growth Regul. 42(1), 7-14. Doi: https://doi.org/10.1023/B:GROW.0000014889.16196.f7
- Naor, V., J. Kigel, M. Ziv, and M. Flaishman. 2004b. A developmental pattern of flowering in colored Zantedeschia spp: effects of bud position and gibberellin. J. Plant Growth Regul. 23(4), 269-279. Doi: https://doi.org/10.1007/BF02637250
- Nery, F.C., V.L.A. Goulart, M.A. Viol, P.D.O. Paiva, R. Paiva, D.O. Prudente and M.C. Nery. 2015. In vitro germination and chemical composition of Zantedeschia aethiopica callus. Acta Hortic. 1083, 189-196. Doi: https://doi.org/10.17660/ActaHortic.2015.1083.22
- Nery, F.C., D.O. Prudente, R. Paiva, M.C. Nery, P.D.O. Paiva, and D. Domiciano. 2018. Micropropagation and histological analysis of calla lily. Acta Hortic. 1224, 183-190. Doi: https://doi.org/10.17660/ActaHortic.2018.1224.24
- Ngamau, K. 2001. Development of an in vitro culture procedure using seeds from Zantedeschia aethiopica 'Green Goddess' as explants. Gartenbauwissenschaft 66(3), 133-139.
- Ngamau, K. 2008. Selection for early flowering, temperature and salt tolerance of Zantedeschia aethiopica 'Green Goddess'. Acta Hortic. 766, 155-162. Doi: https://doi.org/10.17660/ActaHortic.2008.766.19
- Nic-Can, G.I., J.R. Avilez-Montalvo, R.N. Aviles-Montalvo, R.E. Márquez-López, E. Mellado-Mojica, R.M. Galaz-Ávalos, and V.M. Loyola-Vargas. 2016. The relationship between stress and somatic embryogenesis. pp. 151-170. In: Loyola-Vargas, V. and N. Ochoa-Alejo (eds.). Somatic embryogenesis: fundamental aspects and applications. Springer, Cham, Switzerland. Doi: https://doi.org/10.1007/978-3-319-33705-0_9
- Niyokuri, A.N. and S. Nyalala. 2023. Calla lily soft rot causal agents, symptoms, virulence and management: a review. Int. J. Hort. Sci. 29(1), 60-68. Doi: https://doi.org/10.31421/ijhs/29/2023/11380
- Purmale, L., A. Korica, and R. Joffe. 2023. First accounts of Zantedeschia sp Dasheen and Konjac mosaic virus detection in Latvia. Acta Hortic. 1359, 173-178. Doi: https://doi.org/10.17660/ActaHortic.2023.1359.21
- Ribeiro, M.N.O., M. Pasqual, A.B. Silva, and V.A. Rodrigues. 2014. Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina. Ornam. Hortic. 20(1), 21-26. Doi: https://doi.org/10.14295/rbho.v20i1.660
- Rizzo, D., A. Panattoni, L. Stefani, M. Paoli, B. Nesi, S. Lazzereschi, S. Vanarelli, P. Farina, M. Della Bartola, A. Materazzi, and A. Luvisi. 2015. First report of Zantedeschia mild mosaic virus on Zantedeschia aethiopica in Italy. J. Plant Pathol. 97(2), 399.
- Sánchez, J., I. Capote, and M. Daquinta. 2010. Multiplicación in vitro de brotes de tres variedades de callas (Zantedeschia sp.) empleando sistema de inmersión temporal. Cienc. Tecnol. 3(1), 1-5. Doi: https://doi.org/10.18779/cyt.v3i1.90
- Sánchez, J., M. Daquinta, and I. Capote. 2009. Multiplicación in vitro de Zantedeschia spp. variedad Treasure en sistemas de inmersión temporal. Biotecnol. Veg. 9(4), 211-215.
- Schwarz, O.J. and R.M. Beaty. 2000. Organogenesis. pp. 125-138. In: Trigiano, R.N (ed.). Plant tissue culture concepts and laboratory exercises. 2nd ed. Routledge. Doi: https://doi.org/10.1201/9780203743133
- Sheu, S.-Y., C.-W. Huang, M.-Y. Hsu, C. Sheu, and W.-M. Chen. 2017. Cellvibrio Zantedeschiae sp. nov., isolated from the roots of Zantedeschia aethiopica. Int. J. Syst. Evol. Microbiol. 67(9), 3615-3621. Doi: https://doi.org/10.1099/ijsem.0.002178
- Shin, J., S. Bae, and P.J. Seo. 2020. De novo shoot organogenesis during plant regeneration. J. Exp. Bot. 71(1), 63-72. Doi: https://doi.org/10.1093/jxb/erz395
- Singh, M., M.S. Rathore, K. Coudhary, and N.S. Shekhawat. 2009. Direct shoot bud formation and tuberization from aseptically cultured root tubers of calla lily (Zantedeschia aethiopica L.). J. Plant Biochem. Biotechnol. 18(2), 203-207. Doi: https://doi.org/10.1007/BF03263320
- Sun, X., X. Wang, B.S. Subedi, Y. Jiang, D. Wang, R. Gou, G. Zhang, W. Xu, and Z. Wei. 2023. Tissue culture of calla lily (Zantedeschia Spreng.): an updated review on the present scenario and future prospects. Phyton Int. J. Exp. Bot. 92(8), 2413-2428. Doi: https://doi.org/10.32604/phyton.2023.029667
- Sun, X., Y. Wang, T. Yang, X. Wang, H. Wang, D. Wang, H. Liu, X. Wang, G. Zhang, and Z. Wei. 2022. Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.). Front. Gen. 13, 1085694. Doi: https://doi.org/10.3389/fgene.2022.1085694
- Szabó, L.K., F. Desiderio, Z. Kirilla, A. Hegedűs, E. Várallyay, and A. Preininger. 2024. A mini-review on in vitro methods for virus elimination from Prunus sp. fruit trees. Plant Cell Tiss. Organ Cult. 156(42). Doi: https://doi.org/10.1007/s11240-023-02670-9
- Trejo-Téllez, B.I., N.I. Torres-Flores, L.I. Trejo-Téllez, and V.M. Cisneros-Solano. 2013. El alcatraz blanco (Zantedeschia aethiopica (L.) K. Spreng) en el municipio de La Perla, Veracruz. Agro Productividad 6(3). https://revista-agroproductividad.org/index.php/agroproductividad/article/view/457; consulted: May, 2021.
- van der Wolf, J.M., S.H. De Boer, R. Czajkowski, G. Cahill, F. van Gijsegem, T. Davey, B. Dupuis, J. Ellicott, S. Jafra, M. Kooman, I.K. Toth, L. Tsror, I. Yedidia, and J.E. van der Waals. 2021. Management of diseases caused by Pectobacterium and Dickeya species. pp. 175-214. In: van Gijsegem, F., J.M. van der Wolf, and I.K. Toth (eds.). Plant diseases caused by Dickeya and Pectobacterium species. Springer, Cham, Switzerland. Doi: https://doi.org/10.1007/978-3-030-61459-1_6
- Wang, H., Z. Yang, S. Du, L. Ma, Y. Liao, Y. Wang, I. Toth, and J. Fan. 2018. Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence. Mol. Plant Pathol. 19(1), 35-48. Doi: https://doi.org/10.1111/mpp.12493
- Waleron, M., A. Misztak, M. Waleron, M. Franczuk, J. Jońca, B. Wielgomas, A. Mikicinski, T. Popović, and K. Waleron. 2019. Pectobacterium zantedeschiae sp. nov. a new species of a soft rot pathogen isolated from calla lily (Zantedeschia spp.). Syst. Appl. Microbiol. 42(3), 275-283. Doi: https://doi.org/10.1016/j.syapm.2018.08.004
- Wei, Z., H. Zhang, Y. Wang, Y. Li, M. Xiong, X. Wang, and D. Zhou. 2017. Assessing genetic diversity and population differentiation of colored calla lily (Zantedeschia Hybrid) for an efficient breeding program. Genes 8(6), 168. Doi: https://doi.org/10.3390/genes8060168
- Xiao-Chun, S.H.U. 2010. Study on the tissue culture and rapid propagation of Zantedeschia hybrida ‘Feng Yan’. J. Anhui Agr. Sci. 38(26), 14268-14269.
- Yan, L.I., X.T. Wei, Q.H. Gan, J.I. Ying, X.L. Shao, and Y.C. Wang. 2014. Isolation and identification of soft rot bacteria on imported Zantedeschia. J. Food Saf. Qual. 5(12), 3944-3946.