Aplicación conjunta de sustancias húmicas y PGPR inoculadas, y co-inoculadas en plantas de Phaseolus lunatus (L.) yLeucaena leucocephala (Lam.) de Wit
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Resumen
El objetivo de esta investigación fue evaluar el efecto de la inoculación y co-inoculación de rizobios y Azospirillum brasilense combinados con sustancias húmicas (SH) en la promoción del crecimiento de Phaseolus lunatus (frijol lima) y Leucaena leucocephala (leucaena). Para ello se realizaron experimentos en invernadero con el cultivo de cada especie vegetal. Se siguió un diseño experimental de bloques completos al azar con cinco repeticiones. Se sembraron las semillas de plantas y luego se inocularon con rizobios y se coinocularon con A. brasilense. Posteriormente se agregaron SH con la dosis recomendada por el fabricante. En los experimentos con ambas plantas se utilizaron tratamientos control con adición de nitrógeno (N) con o sin SH. Después de 45 días, se determinó la masa seca de la parte aérea (MSPA), la masa seca de la raíz (MSR), el N acumulado en la parte aérea (Nac) y el índice de eficiencia relativa (IER). Además, se determinó la masa de nódulos secos (MNS) y en las plantas de leucaena el número de nódulos (NN). Los resultados mostraron que en las plantas de frijol lima y leucaena hubo un mayor aumento de MSPA, MSR y Nac en los tratamientos que recibieron SH y co-inoculación con rizobios y A. brasilense que en los tratamientos que fueron inoculados solo con rizobios y SH, y en tratamientos que recibieron N y SH en comparación con la adición de N y la inoculación aislada de rizobios. La aplicación combinada de SH y rizobios en co-inoculación con A. brasilense tuvo un mayor efecto en el aumento de MNS en frijol lima y NN en leucaena, seguido de los tratamientos donde solo se agregaron rizobios con SH. Estos resultados indican la existencia de una interacción potencial del uso de SH con la co-inoculación de rizobios y A. brasilense, lo que resulta prometedor para la producción de cultivos agrícolas sostenibles.
Palabras clave
Sustancias húmicas, Co-inoculación, Rizobios, Azospirillum, Bioestimulantes, Sostenibilidad
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