Impatiens Walleriana: Prospects For Genetic Improvement

Impatiens walleriana: perspectivas para el mejoramiento genético

Main Article Content

Abstract

Impatiens walleriana es una planta de la familia Balsaminaceae con alto valor ornamental. Presenta flores muy atractivas, las cuales se pueden encontrar en diferentes formas y colores, debido a ello, forma parte de muchos jardines en un gran número de países alrededor del mundo. El mejoramiento genético en esta especie usualmente se ha llevado a cabo mediante métodos convencionales, sin embargo, el avance de la tecnología ha hecho posible la utilización de un gran número de herramientas biotecnológicas y moleculares. El objetivo de esta revisión es consolidar las bases para un programa de mejora genética en I. walleriana. En esta revisión se abarcan diferentes aspectos que son básicos para incurrir en la mejora genética de esta especie; dichos factores son la morfología floral, polinización, cariotipado y estudios de variabilidad genética. Además, se expone lo que se conoce hasta la fecha sobre estudios enfocados en genes implicados en los procesos de estrés ante la sequía, tolerancia a Plasmopara obducens y longevidad floral.

Keywords:

Downloads

Download data is not yet available.

Article Details

References (SEE)

Abrahamczyk S., Lozada-Gobilard S., Ackermann M., Fischer E., Krieger V., Redling A. y Weigend M. (2017). A question of data quality – testing pollination syndromes in Balsaminaceae. PLoS One, 12(10), e0186125. DOI: https://doi.org/10.1371/journal.pone.0186125

Adamowski W. (2008). Balsams on the offensive: the role of planting in the invasion of Impatiens species. In Tokarska-Guzik B., Brock J. H., Brundu G., Child L., Daehler C. C. y Pyšek P. (eds), Plant invasions: human perception, ecological impacts and management (pp. 57-70). Leiden, Netherlands: Backhuys Publishers.

Aldon D., Mbengue M., Mazars C. y Galaud J. P. (2018). Calcium signaling in plant biotic interactions. Int. J. Mol. Sci., 19(3), 665. DOI: https://doi.org/10.3390/ijms19030665

Antonic D., Miloševic S., Cingel A., Lojic M., Trifunovic-Momcilov M., Petric M. y Simonovic A. (2016). Effects of exogenous salicylic acid on Impatiens walleriana L grown in vitro under polyethylene glycol-imposed drought. S. Afr. J. Bot., 105, 226-233.

Arisumi T. (1973). Chromosome numbers and interspecific hybrids among New Guinea Impatiens species. J. Hered., 64(2), 77-79.

Barrett B.A., Kidwell K.K. y Fox P.N. (1998). AFLP vs. pedigree based genetic diversity assessment methods. Crop Sci., 38, 1271-1278.

Bhattarai K., Wang W., Cao Z. y Deng Z. (2018). Comparative Analysis of Impatiens Leaf Transcriptomes Reveal Candidate Genes for Resistance to Downy Mildew Caused by Plasmopara obducens. Int. J. Mol. Sci., 19(7), 2057. DOI: https://doi.org/10.3390/ijms19072057

Bicknell R. (1995). Breeding cut flower cultivars of Leptospermum using interspecific hybridization. New Zeal J Crop Hortic., 23, 412-421.

Bigeard J., Colcombet J. y Hirt H. (2015). Signaling Mechanisms in Pattern-Triggered Immunity (PTI). Molecular Plant., 8(4), 521-539. DOI: https://doi.org/10.1016/j.molp.2014.12.022

Blanco J. y Vázquez F.M. (2014). Impatiens balfourii Hook (Balsaminaceae) actuando como especie invasora en la Reserva Natural de la Garganta de los Infiernos (Extremadura). Bouteloua, 18, 100-105.

Broome R., Sabir K. y Carrington S. (2007). Plants of the Eastern Caribbean. Online database. University of the West Indies, Barbados. http://ecflora.cavehill.uwi.edu/index.html

CABI. (2019). Invasive Species Compendium. https://www.cabi.org/isc/datasheet/28769#tosummaryOfInvasiveness

Carr J. y Korban S.S. (2004). Evaluating genetic relationships in seed impatiens, Impatiens walleriana, using AFLP profiling. Plant Breeding, 123, 577-581.

Carrodeguas A. y Zúñiga, A. (2020). Bases para la mejora genética en Gerbera hybrida. Repertorio Científico, 23(2), 62-51.

Casanova E., Trillasa M.I., Moyseta L.L., Vainstein A. (2005). Influence of rol genes in floriculture. Biotechnology Advances, 23, 3-39.

Chavan S. K., Singh A. y Barkule S. (2018). Genetic variability studies on Adenium obesum Forssk Roem & Schult. Asian. Jr. of Microbiol. Biotech. Env. Sc., 20(3), 965-969.

Cubero J.I. (2003). Los poliploides en la mejora vegetal. In: Introducción a la mejora genética vegetal. Cap. 15. Ediciones Mundi-prensa. Madrid. España, 2003. p. 325-351.

Cunnington J.H., Aldaoud R., Loh M., Washington W.S. y Irvine G., (2008). First record of Plasmopara obducens (downy mildew) on impatiens in Australia. Plant Pathology, 57(2), 371. DOI: https://doi.org/10.1111/j.1365-3059.2007.01630.x

DAISIE (2018). Delivering Alien Invasive Species Inventories for Europe. http://www.europe-aliens.org/

Dan Y., Baxter A., Zhang S., Pantazis C. y Veilleux, R. (2010). Development of Efficient Plant Regeneration and Transformation System for Impatiens using Agrobacterium tumefaciens and multiple bud cultures as explants. BMC Plant Biol., 10: 165. DOI: https://doi.org/10.1186/1471-2229-10-165

Daughtrey M., Beckerman J., Davis W.J., Rane K., Crouch J.A. (2020). Corroboration That Highly Resistant Impatiens Cultivars Are Not Immune to Downy Mildew Disease: A Report of Crop Losses from Two California Producers. Plant Health Progress, 21(3): 214-216.

De La Riva F. (2011). Poscosecha de flores de corte y medio ambiente. IDESIA, 29(3), 125-130

de Morais J.S., Sant’Ana A.S., Dantas A.M., Silva B.S., Lima M.S., Borges G.C. y Magnani M. (2020). Antioxidant activity and bioaccessibility of phenolic compounds in white, red, blue, purple, yellow and orange edible flowers through a simulated intestinal barrier. Food Res. Int., 131, 109046. DOI: https://doi.org/10.1016/j.foodres.2020.109046

Delteil A., Gobbato E., Cayrol B., Estevan J., Michel-Romiti C., Dievart A., Kroj T. y Morel J.B. (2016). Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus. BMC Plant Biology, 16, 17. DOI: https://doi.org/10.1186/s12870-016-0711-x

Demidchik V. (2015). Mechanisms of oxidative stress in plants: From classical chemistry to cell biology. Environ. Exp. Bot.,109, 212-228.

Díez M.J. y Nuez F. (2008). Tomato. In: J. Prohens-Tomás y J. Nuez (eds), Vegetables II: Fabaceae, Liliaceae, Solanaceae, and Umbelliferae. Handbook of Plant Breeding. Ed. Springer Publishing. NY, USA.

Diningsih E., Yanda R.P. Soehendi R. y Marwoto B. (2019). Identification of the main virus infecting impatiens (Impatiens balsamina L.) IOP Conference Series: Earth and Environmental Series. Southeast Asia Plant Protection Conference. Bongor, Indonesia

Djuric M., Subotic A., Prokic L., Trifunović-Momčilov M., Cingel A., Dragićević M., Simonovic A. y Milosevic S. (2021). Molecular characterization and expression of four aquaporin Genes in Impatiens walleriana during drought stress and recovery. Plants, 10(1), 154. DOI: https://doi.org/10.3390/plants10010154

Djuric M., Subotic A., Prokic L., Trifunović-Momčilov M., Cingel A., Vujicic M. y Milosevic S. (2020). Morpho-physiological and molecular evaluation of drought and recovery in Impatiens walleriana grown ex vitro. Plants, 9(11), 1559. DOI: https://doi.org/10.3390/plants9111559

Antonić, D.D., Subotic A.R., Dragićević M.B., Pantelic D., Milosevic S.M., Simonovic A.D.y Momčilović I. (2020). Effects of exogenous salicylic acid on drought response and characterization of dehydrins in Impatiens walleriana. Plants, 9(11), 1589. DOI: https://doi.org/10.3390/plants9111589

Fischer E. y Rahelivololona M.E. (2002). New taxa of Impatiens (Balsaminaceae) from Madagascar I. Adansonia, 24, 271-294.

Fischer E. y Rahelivololona M.E. (2016). New taxa of Impatiens (Balsaminaceae) from Madagascar VIII. Impatiens maxhuberi, a new species from Marojejy and Anjanaharibe-Sud. Phytotaxa, 244, 191-195.

Fort A. (2013). Genome dosage and parent of origin effects in a ploidy series of Arabidopsis thaliana (PhD Diss.). National University Ireland, Galway. https://aran.library.nuigalway.ie/handle/10379/4432

Fujihashi H., Akiyama S. y Ohba H. (2002). Origin and relationships of the Sino-Himalayan Impatiens (Balsaminaceae) based on molecular phylogenetic analysis, chromosome numbers and gross morphology. J. Jap. Bot. 77, 284-295.

Fukumoto H., Yamaki M., Isoi K. y Ishiuro K. (1996). Antianaphylactic Effects of the Principal Compounds from the White Petals of Impatiens balsamina L. Phytother Res., 10: 202-206.

Gechev T.S., Van-Breusegem F., Stone J.M., Denev I. y Laloi C. (2006). Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bioessays, 28, 1091-1101. DOI: https://doi.org/10.1002/bies.20493

Ghanbari M., Jowkar A., Salehi H. y Zarei M. (2019). Effects of polyploidization on petal characteristics and optical properties of Impatiens walleriana (Hook.). Plant Cell Tissue and Organ Culture, 138, 299-310. DOI: https://doi.org/10.1007/s11240-019-01625-3

Granados C. y Chaparro-Giraldo A. (2012). Métodos de transformación genética de plantas. Revista UDCA Actualidad y Divulgacion Cientifica. 15, 49-61. DOI: https://doi.org/10.31910/rudca.v15.n1.2012.802

Grey-Wilson C. (1980). Impatiens of Africa: Morphology, pollination and pollinators, ecology, phytogeography, hybridization, keys and a systematic treatment of all African species with a note on collecting and cultivation. Ed. University of Chicago Press.

GRIIS (2018). Global Register of Introduced and Invasive Species. IUCN ISSG. http://griis.org/

Gutiérrez-Beltrán E. y de la Torre F.N. (2015). La coevolución como un sistema de defensa en la interacción planta-patógeno. Encuentros en la biología, 9(157), 79-81.

Haddad B., Gristina A.S., Mercati F., Saadi A.E., Aiter N., Martorana A., Sharaf A. y Carimi F. (2020). Molecular analysis of the official Algerian olive collection highlighted a hotspot of Diversity in the Central Mediterranean basin. Genes, 11(3), 303. DOI: https://doi.org/10.3390/genes11030303

Herrera J.C. (2007). La citogenética molecular y su aplicación en el estudio de los genomas vegetales. Agronomía Colombiana, 25(1), 26-35.

Howard N.P., Stimart D., de Leon N., Havey M.J. y Martin W. (2012). Diallel analysis of floral longevity in Impatiens walleriana. J Am Soc Hortic., 137, 47-50.

Janssens S.B., Geuten K., Viaene T., Yuan Y.M., Song Y. y Smets E. (2007). Phylogenetic utility of the AP3/DEF K-domain and its molecular evolution in Impatiens (Balsaminaceae). Mol. Phylogenet. Evol., 43(1), 225-239.

Janssens S.B., Geuten K., Yuan Y.M., Song Y., Kupfer P. y Smets E. (2006). Phylogenetics of Impatiens and Hydrocera (Balsaminaceae) using chloroplastat pB-rbcL spacer sequences. Syst. Bot., 31, 171-180.

Janssens S.B., Knox E.B., Huysmans S., Smets E.F. y Merckx V.F.S.T. (2009). Rapid radiation of Impatiens (Balsaminaceae) during Pliocene and Pleistocene: result of a global climate change. Mol. Phylogenet. Evol., 52, 806-824.

Janssens S.B., Wilson S.Y., Yuan Y.M., Nagels A., Smets E.F. y Huysmans S. (2012). A total evidence approach using palynological characters to infer the complex evolutionary history of the Asian Impatiens (Balsaminaceae). Taxon, 61, 355-367

Jones D. y O´Neill T. (2004). Impatiens downy mildew. East Malling, UK: Horticultural Development Council: Factsheet 05/04 Impatiens Protected Crops.

Jones K. y Smith J.B. (1966). The cytogeography of Impatiens L. (Balsaminaceae). Kew Bull., 20(1): 63-72.

Lane C.R., Beales P.A., O'Neill T.M., McPherson G.M., Finlay A.R., David, J., Constantinescu O. y Henricot B. (2005). First report of Impatiens downy mildew (Plasmopara obducens) in the UK. Plant Pathology, 54(2), 243. DOI: https://doi.org/10.1111/j.1365-3059.2005.01133.x

Lehrer J., Brand M. y Lubell J. (2008). Induction of tetraploidy in meristematically active seeds of Japanese barberry (Berberis thunbergii var. atropurpurea) through exposure to colchicine and oryzalin. Scientia Hort., 119, 67-71.

Lodish H., Berk A., Matsudaira P., Kaiser C., Krieger M., Scott M.P., Zipursky L. y Darnell J.(2016). Cap 12. Cellular energetics. In: Molecular Cell Biology. Ed. W.H Freeman and Company. NY, USA.

Mabberley D.J. (2017). Mabberley’s Plant-Book, A portable dictionary of plants, their classification and uses, 4th ed. Cambridge University Press, UK.

Macnish A.J., Irving D.E., Joyce D.C., Vithanage V., Wearing A.H. y Lisle A.T (2004). Variation in ethylene-induced postharvest flower abscission responses among Chamelaucium Desf. (Myrtaceae) genotypes. Sci Hortic., 102, 415-432. DOI: https://doi.org/10.1016/j.scienta.2004.05.002

Mercati F. y Sunseri F. (2020). Genetic Diversity Assessment and marker-assisted selection in crops. Genes, 11, 1481. DOI: https://doi.org/10.3390/genes11121481

Merling C. y Grant M.F. (1986). Hybridization studies in the genus Impatiens. Canadian Journal of Botany, 64, 5. DOI: https://doi.org/10.1139/b86-145

Messina C.D., Podlich D. y Dong Z. (2011). Yield-trait performance landscapes: from theory to aplication in breeding maize for drought tolerance. J. Exp. Bot., 62(3), 855-868. DOI: https://doi.org/10.1093/jxb/erq329

Milošević S., Lojić M., Antonić D., Cingel A. y Subotić A. (2015). Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation. Genetika, 47(1), 71-84.

Mondragón J. (2009). Impatiens walleriana Hook f. (Balsaminaceae): ficha informativa. http://www.conabio.gob.mx/malezasdemexico/balsaminaceae/impatiens-walleriana/fichas/ficha.htm#:~:text=Flores%3A%20De%20varios%20colores%3A%20rojas,de%20largo%3B%20p%C3%A9talos%20relativamente%20similares

Motz V.A., Bowers C.P., Kneubehl A.R., Lendrum E.C., Young L.M. y Kinder D.H. (2015). Efficacy of the saponin component of Impatiens capensis Meerb. in preventing urushiol-induced contact dermatitis. J Ethnopharmacol, 162, 163-167.

Navarro C. y Muñoz-Garmendia F. (2013) Flora Ibérica, vol. IX: Impatiens L. http://www.floraiberica.es/floraiberica/texto/imprenta/tomoIX/09_126_00_01_Balsaminaceae_2010_09 20.pdf

Naveed Z.A. y Ali G.S. (2018). Comparative transcriptome analysis between a resistant and a susceptible wild tomato accession in response to Phytophthora parasitica. Int. J. Mol. Sci., 19(2), 3735. DOI: https://doi.org/10.3390/ijms19123735

Ojito-Ramos K. y Portal O. (2010). Introducción al sistema inmune en plantas. Biotecnología Vegetal, 10(1), 3-19.

Olsen A., Lutken H., Nymark J., Muller R. (2015). Ethylene resistance in flowering ornamental plants – improvements and future perspectives. Horticulture Research, 2, 15038. DOI: https://doi.org/10.1038/hortres.2015.38

Onozaki T., Yagi M., Tanase K. y Shibata M. (2011). Crossings and selections for six generations based on flower vase life to create lines with ethylene resistance or ultra-long vase life in carnations (Dianthus caryophyllus L.). J. Jpn. Soc. Hortic. Sci., 80, 486-498.

Oswald B. y Nuismer S. (2007). Neopolyploidy and pathogen resistance. Proc. Biol. Sci., 274, 2393-2397.

Palumbo F., Galvao A.C., Nicoletto C., Sambo P. y Barcaccia G. (2019). Diversity analysis of sweet potato genetic resources using morphological and quantitative traits and molecular markers. Genes, 10(11), 840. DOI: https://doi.org/10.3390/genes10110840

Parker A. (2005). Pollinator limitation and seed set of Impatiens walleriana. Tropical Ecology Collection, Monteverde Institute. Digital Colections. https://digital.lib.usf.edu/SFS0001528/00001

PIER (2018). Pacific Islands Ecosystems at Risk. In: Pacific Islands Ecosystems at Risk. Honolulu, Hawaii, USA: HEAR, University of Hawaii. http://www.hear.org/pier/index.html

Pires E.d.O., Pereira E., Pereira C., Dias M.I., Calhelha R.C., Ćirić A., Soković M., Hassemer G., Garcia C.C., Caleja C., Barros L. y Ferreira I.C.F.R. (2021). Chemical Composition and Bioactive Characterization of Impatiens walleriana. Molecules, 26, 1347. DOI: https://doi.org/10.3390/molecules26051347

Pyšek P. y Prach K. (1993). Plant invasions and the role of riparian habitats: a comparison of four species alien to central Europe. Journal of Biogeography, 20: 413-420.

Queensland Government (2018). Weeds of Australia, Biosecurity Queensland Edition. In: Weeds of Australia, Biosecurity Queensland Edition. Australia: Queensland Government. http://keyserver.lucidcentral.org/weeds/data/media/Html/search.html

Ranty B., Aldon D., Cotelle V., Galaud J.P., Thuleau P., Mazars C. (2016). Calcium Sensors as Key Hubs in Plant Responses to Biotic and Abiotic Stresses. Frontiers in Plant Science. DOI: https://doi.org/10.3389/fpls.2016.00327

Rao A., Bakhsh A., Kiani S., Shahzad K., Shahid A., Husnain T. y Riazuddin S. (2009). The myth of plant transformation. Biotechn. Adv., 27, 753-763.

Renganayaki K., Read J.C. y Fritz A.K. (2001). Genetic diversity among Texas bluegrass genotypes (Poa arachnifera Torr.) revealed by AFLP and RAPD markers. Theor. Appl. Genet. 102, 1037-1045.

Rommens C. (2004). All-native DNA transformation: a new approach to plant genetic engineering. Trends Plant Sci., 9(9), 457-464.

Sanjari A. y Yazdansepas A. (2008). Evaluation of Wheat (Triticum aestivum L.) Genotypes under Pre- and Post-anthesis Drought Stress Conditions. J. Agric. Sci. Technol., 10, 109-121.

Shajita P., Dhanesh N., Ebin P., Joseph L., Devassy A., John R. y Mathew L. (2015). Molecular phylogeny of Balsams (Genus Impatiens) based on ITS regions of nuclear ribosomal DNA implies two colonization events in south India. Journal of Applied Biology & Biotechnology, 4(6), 1-9. DOI: https://doi.org/10.7324/JABB.2016.40601

Yua S.X., Steven J.B., Zhua X.Y, Magnus L., Gao T.G. y Wei W. (2015). Phylogeny of Impatiens (Balsaminaceae): Integrating molecular and morphological evidence into a new classification. Cladistics, 32(2), 179-197. DOI: https://doi.org/10.1111/cla.12119

Shibuya K., Berry K.G., Ciardi J.A., Loucas H.M., Underwood B.A., Nourizadeh J.R., Ecker H.J. y Klee D.G. (2004). The central role of PhEIN2 in ethylene responses throughout plant development in petunia. Plant Physiol, 136, 2900-2912.

Song Y., Yong-Ming Y. y Philippe K. (2003). Chromosomal evolution in Balsaminaceae, with cytological observations on 45 species from Southeast Asia. Caryologia, 56, 463-481.

Sreekala A.K., Pandurangan A.G., Ramasubbu R., Kulloli S.K. (2008). Reproductive biology of Impatiens coelotropis Fischer, a critically endangered balsam from the Southern Western Ghats. Current Science. 95(3), 386-388.

Tanaka Y., Katsumoto Y., Brugliera F. y Mason J. (2005). Genetic engineering in floriculture. Plant Cell, Tissue and Organ Culture, 80, 1-24.

Tanase K., Otsu S., Satoh S., Onozaki T. (2013). Expression and regulation of senescence related genes in carnation flowers with low ethylene production during senescence. J. Jpn. Soc. Hortic. Sci., 82, 179-187.

Tang D., Wang G. y Zhou J. (2017). Receptor kinases in plant-pathogen interactions: more than pattern recognition. Plant Cell, 29, 618-637. DOI: https://doi.org/10.1105/tpc.16.00891

Tatsuzawa F., Suzuki Y., Sato M., Kato K. (2016). Flower Colors and Pigments in the Cultivars of Impatiens walleriana. Horticultural Research (Japan), 15, 115-122. DOI: https://doi.org/10.2503/hrj.15.115

Taylor N.y Fauquet C. (2002). Microparticle bombardment as a tool in plant science and agricultural biotechnology. DNA and Cell Biology. (USA), 21(12), 963-977.

Uchneat M.S. (2007). Impatiens. En: Anderson N.O. (eds) Flower Breeding and Genetics. Springer, Dordrecht. DOI: https://doi.org/10.1007/978-1-4020-4428-1_10

USDA-NRCS (2018). The PLANTS Database. En: The PLANTS Database Greensboro, North Carolina, USA: National Plant Data Team. https://plants.sc.egov.usda.gov

Van-Alvorst A.C. y Bovy A.G. (1995). The role of ethylene in the senescence of carnation flowers, a review. Plant Growth Regulation, 16: 43-53.

van Vankelburg J.L.C.H., Schoenenber N., van de Vossenberg B.T.L.H., Man in´t Veld W.A., Westenberg M. y Boer E. (2019). A natural hybrid of Impatiens, in the introduced range, demonstrated by sequence analysis of the nuclear ribosomal DNA-gene repeat. Biodiversity & Conservation Biology, 166(2), 144-152. DOI: https://doi.org/10.1080/23818107.2019.1584863

Wang W., He Y., Cao Z. y Deng Z. (2018). Induction of Tetraploids in Impatiens (Impatiens walleriana) and Characterization of Their Changes in Morphology and Resistance to Downy Mildew. HortScience, 53(7), 925-931. DOI: https://doi.org/10.21273/HORTSCI13093-18

Wang Y. y LI J. (2006). Genes controlling plant architecture. Current opinion on Biotech, 17, 123-129.

Wegulo S.N., Koike S.T., Vilchez M. y Santos P., (2004). First report of downy mildew caused by Plasmopara obducens on Impatiens in California. Plant Disease, 88(8), 909. DOI: https://doi.org/10.1094/PDIS.2004.88.8.909B

Yšek P. (1995). Invasion dynamics of Impatiens glandulifera. A century of spreading reconstructed. Biological Conservation, 74, 41-48.

Yuan Y.M., Song Y., Geuten K., Rahelivololona E., Wohlhauser S., Fischer E., Smets E. y Kaupfer P. (2004). Phylogeny andbiogeography of Balsaminaceae inferred from ITS sequence data. Taxon, 53, 391-403.

Yue B., Xue W., Luo L. y Xing J.Y. (2008). Identification of quantitative trait loci for four morphologic traits under water stress in rice (Oryza sativa L.) Genet. Genomics, 35(9), 569-575. DOI: https://doi.org/10.1016/S1673-8527(08)60077-6

Zhu Y., Shao J., Zhou Z. y Davis R.E. (2017). Comparative transcriptome analysis reveals a preformed defense system in apple root of a resistant genotype of G.935 in the absence of pathogen. Int. J. Plant Genomics. DOI: https://doi.org/10.1155/2017/8950746

Zhuo F., Xiong F., Deng X., Li Z. y Ren M. (2020). Target of Rapamycin (TOR) Negatively Regulates Ethylene Signals in Arabidopsis. Intern. Journ. Mol. Sciences. DOI: https://doi.org/10.3390/ijms21082680

Zúñiga A. y Carrodeguas A. (2020). Factores importantes para el mejoramiento genético en la piña (Anannas comosus var. comosus). Repertorio Científico, 23(2), 50-37.

Citado por: