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Vol. 15 No. 1 (2024)
Vol 15, Núm.1 (2024): Enero-Junio

Artículos de investigación / Research papers

Antioxidant activity of non-psychoactive cannabis varieties from North Cauca, Colombia

https://doi.org/10.19053/01217488.v15.n1.2024.15214

Published 2024-02-13

  • Paola Andrea Arango Romero
    • ,
  • Jhan Carlos Delgado
    • ,
  • Sandra Marcela Muñoz
    • ,
  • Luis Llanos
    • ,
  • Ricardo Benítez

Paola Andrea Arango Romero
Universidad del Cauca

Jhan Carlos Delgado
Universidad del Cauca

Sandra Marcela Muñoz
Universidad del Cauca

Luis Llanos
Universidad del Cauca

Ricardo Benítez
Universidad del Cauca

Abstract

The present study aim to publicize the chemical composition and possible medicinal potential of varieties of non-psychoactive cannabis grown in the department of Cauca. Cannabinoids were identified and quantified by high-performance liquid chromatography coupled to an ultraviolet detector (HPLC/UV) for the analysis of the flower, and gas chromatography coupled to a mass spectrometer (GC-MS) for the analysis of the ethanolic extracts and content terpenes. Phenols were quantified by reaction with the Folin & Ciocalteau reagent; for the determination of flavonoids and anthraquinones, the extracts were treated with AlCl3. Finally, to determine the antioxidant activity, three methods were used: DPPH, ABTS and FRAP. It was possible to determine that varieties A and B contained percentages of total tetrahydrocannabinol (THC) less than 1% and percentages of total cannabidiol (CBD) between 9-15%. In the ethanolic extracts concentrations (m/m) of CBD were reached in varieties A and B, of 10% and 13.7%, respectively. Nine terpenes from sample A and seven from sample B were identified and quantified, with β-caryophyllene being the most abundant in both. Considering that there is evidence in the literature that the CBD/THC ratio influences biological activity, ethanolic extracts of varieties A and B are expected to have moderate to low antioxidant activity, which, according to some researchers, it may be associated with the neuroprotective effect, which may be favored by the presence of β-caryophyllene.

Keywords

Antioxidant activity, Cannabinoid, Cannabis, Terpenoid

PDF (Español)

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