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Vol. 15 Núm. 1 (2024)
Vol 15, Núm.1 (2024): Enero-Junio

Artículos de investigación / Research papers

Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática

https://doi.org/10.19053/01217488.v15.n1.2024.15304

Publicado 2024-04-09

Juan Alejandro Pérez Aguilar
a:1:{s:5:"es_ES";s:21:"Universidad del Cauca";}

Ricardo Benítez Benítez
Universidad del Cauca

Resumen

Cannabis sativa es una planta ampliamente utilizada en Colombia para propósitos medicinales, para lo que se usa su flor, generando altas cantidades de tallos residuales. En este estudio, tallos de Cannabis se pretrataron con ácido sulfúrico o hidróxido de sodio diluido y su efecto se analizó por espectroscopía de infrarrojo (FTIR-ATR). Los sólidos remanentes se llevaron a hidrólisis enzimática usando dos mezclas enzimáticas comerciales: Celluclast 1.5 L y Cellic CTec3; se hizo seguimiento a la producción de azúcares reductores. Los pretratamientos removieron hemicelulosa y lignina, evidenciado por la reducción en las señales en 1734, 1540 y 1240 cm-1. También ocasionaron un incremento en la cristalinidad de la celulosa. Ambos pretratamientos lograron aumentar la producción de azúcares en la hidrólisis con las dos enzimas trabajadas. Aunque el pretratamiento alcalino fue mucho más efectivo que el ácido y permitió producir hasta 28.59 mg/mL de azúcares con Celluclast 1.5 L y 24.94 mg/mL con Cellic CTec3. El mezclar estas enzimas, usarlas de forma secuencial, o incrementar la carga enzimática no tuvo un efecto sobre la concentración final de azúcares alcanzada.

Palabras clave

Cannabis sativa, cristalinidad, azúcares reductores, hidrólisis enzimática, pretratamientos

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