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Vol. 18 No. 3 (2024)
In press / En imprenta

Fruits section

Occurrence and molecular characterization of cucumber mosaic virus (CMV) in plantain orchards in Caldas and Risaralda departments of Colombia

https://doi.org/10.17584/rcch.2024v18i3.17841

Published 2024-09-01

  • Diana Milena Rodríguez-Mora
    • ,
  • Luz Natalia Martínez-Caballero
    • ,
  • Mónica Betancourt
    • ,
  • Isabel Moreno

Diana Milena Rodríguez-Mora
Corporación Colombiana de Investigación Agropecuaria – Agrosavia, Centro de Investigación Palmira, Palmira (Colombia)

Luz Natalia Martínez-Caballero
Corporación Colombiana de Investigación Agropecuaria – Agrosavia, Centro de Investigación Palmira, Palmira (Colombia)

Mónica Betancourt
Corporación Colombiana de Investigación Agropecuaria – Agrosavia, Centro de investigación Tibaitatá, Mosquera (Colombia)

Isabel Moreno
Corporación Colombiana de Investigación Agropecuaria – Agrosavia, Centro de Investigación Palmira, Palmira (Colombia)

Chlorotic streaking and mosaic in plantain leaf. Photo: M. Betancourt

Abstract

Cucumber mosaic virus (CMV) affects plantain (Musa AAB) orchards in most producing regions across the globe. This study aimed to determine the occurrence and characterize the CMV affecting plantain orchards in the departments of Caldas and Risaralda, Colombia. Leaf tissue samples were collected from six municipalities, then they were screened for CMV diagnosis using the enzyme-linked immunosorbent assay (ELISA). The samples with positive reaction were confirmed by reverse transcription polymerase chain reaction (RT-PCR). The results confirmed the presence of CMV with an incidence of 2.8% in the region in study. For the molecular characterization of the virus, a partial region of the RNA-dependent RNA polymerase (RdRp) gene was amplified and sequenced. The sequence analysis showed that the isolates shared nucleotide identities above 97.7% with the GenBank accession MG696855.1, corresponding to a CMV isolate obtained from lulo (Solanum quitoense) in Colombia. Phylogenetic analysis grouped the sequences generated in this study with sequences of reference to the subgroup IA. Our results contribute to the update and understanding of CMV population dynamics in this important plantain production region.

Keywords

CMV, ELISA, Molecular detection, Musa AAB

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References

  1. Alarcón, J.J., J. Castaño, M. Aristizábal, and M. Betancurt. 2005. Reconocimiento de enfermedades virales en plátano y banano en la granja Montelindo, municipio de Palestina (Caldas), Colombia. pp. 238-241. In: Castaño, J. (ed.). Memorias II Seminario Internacional Sobre Producción, Comercialización e Industrialización de Plátano. Corpoica, Manizales, Colombia.
  2. Anderson, B.J., P.M. Boyce, and C.L. Blanchard. 1995. RNA 4 sequences from cucumber mosaic virus subgroup I and II. Gene 161(2), 293-294. Doi: https://doi.org/10.1016/0378-1119(95)00276-C
  3. Apalowo, O.A., A.O. Adediji, O.S. Balogun, T.I. Fakolujo, J.M. Archibong, N.B. Izuogu, M.A. Abdelgawad, M.M. Ghoneim, S. Mustapha, F.S.I. Qashqari, G.E. Batiha, and G.I. Atiri. 2022. Genetic structure of cucumber mosaic virus from natural hosts in Nigeria reveals high diversity and occurrence of putative novel recombinant strains. Front. Microbiol, 13, 753054. Doi: https://doi.org/10.3389/fmicb.2022.753054
  4. Bae, M., H.-J. Lee, S. Joo, B. Kim, M.-R. Park, and R.-D. Jeong. 2021. First report of cucumber mosaic virus infecting banana (Musa spp.) in Korea. J. Plant Pathol. 103(3), 1075. Doi: https://doi.org/10.1007/s42161-021-00882-6
  5. Belalcázar, S., M.I. Arcila, J.A. Valencia, H. Reichel, and J. Narváez. 1996. Efecto del virus del mosaico del pepino, CMV, sobre los parámetros de crecimiento, desarrollo y producción del clon de banano Gros Michel. pp. 88-94. In: Tecnología del Eje Cafetero para la siembra y explotación rentable del cultivo del plátano. Comité Departamental de Cafeteros del Quindío, Armenia, Colombia.
  6. Bello, V.H., L. Carpim, H.J. Lorenzi, J.A.M. Rezende, and E.W. Kitajima. 2023. Natural occurrence of cucumber mosaic virus infecting Physalis peruviana plants in Brazil. J. Phytopathol. 171(4-5), 143-144. Doi: https://doi.org/10.1111/jph.13165
  7. Bhat, A.I., T. Hohn, and R. Selvarajan. 2016. Badnaviruses: the current global scenario. Viruses 8(6), 177. Doi: https://doi.org/10.3390/v8060177
  8. Buitrón-Bustamante, J.L. and L.E. Morillo-Velastegui. 2017. Estandarización de un método de detección molecular del Cucumber mosaic virus (CMV) en banano ecuatoriano. Rev. Corpoica Cienc. Tecnol. Agropecuaria 18(1), 113-124. Doi: http://doi.org/10.21930/rcta.vol18_num1_art:562
  9. Cabrera, D., M. Jaramillo, J. Villarreal, C. Nome, C. Torres, O. Portal, and F. Giolitti. 2018. Cucumber mosaic virus infecting ‘Cavendish’ banana in Argentina. Australasian. Plant Dis. Notes 13, 36. Doi: http://doi.org/10.1007/s13314-018-0322-5
  10. Catellanos, F.G. and J.C. Lucas. 2011. Caracterización física del fruto en variedades de plátano cultivadas en la zona cafetera de Colombia. Acta Agron. 60(2), 176-182.
  11. Chang, S., J. Puryear, and J. Cairney. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Mol. Biol. Rep. 11, 113-116. Doi: https://doi.org/10.1007/BF02670468
  12. Du, Z., J. Chen, and C. Hiruki. 2006. Optimization and application of a multiplex RT-PCR system for simultaneous detection of five potato viruses using 18S rRNA as an internal control. Plant Dis. 90(2), 185-189. Doi: https://doi.org/10.1094/PD-90-0185
  13. Fidan, H. and G. Koç. 2019. Occurrence, ecology, and phylogeny of banana streak badnavirus (BSV) and cucumber mosaic cucumovirus (CMV) in Musa sp. production areas of the Mediterranean coastline of Turkey. Appl. Ecol. Environ. Res. 17(3), 5935-5951 Doi: https://doi.org/10.15666/aeer/1703_59355951
  14. Ganchozo-Mendoza, E., A.F.F. Quadros, F.M. Zerbini, F.J. Flores, and F.R. Garcés-Fiallos. 2024. Characterization and genome analysis of Cucumber mosaic virus on commercial tobacco plants in Ecuador. Trop. Plant Pathol. 49, 480-488. Doi: https://doi.org/10.1007/s40858-024-00643-7
  15. Gauhl, F., C. Pasberg-Gauhl, B.E.L. Lockhart, J. d'A. Hughes, and G. Dahal. 1999. Incidence and distribution of banana streak badnavirus in the plantain production region of southern Nigeria. Int. J. Pest Manag. 45(3), 167-171. Doi: https://doi.org/10.1080/096708799227752
  16. Grieco, F., R. Alkowni, M. Saponari, V. Savino, and G.P. Martelli. 2000. Molecular detection of olive viruses. EPPO Bull. 30(3-4), 469-473. Doi: https://doi.org/10.1111/j.1365-2338.2000.tb00931.x
  17. Hayes, R.J. and K.W. Buck. 1990. Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63(2), 363-368. Doi: https://doi.org/10.1016/0092-8674(90)90169-F
  18. Jacquemond, M. 2012. Cucumber mosaic virus. Adv. Virus Res. 84, 439-504. Doi: https://doi.org/10.1016/B978-0-12-394314-9.00013-0
  19. James, A.P., D.B. Kidanemariam, S.D. Hamill, J.L. Dale, and R.M. Harding. 2021. Infectivity of an infectious clone of banana streak CA virus in A-genome bananas (Musa acuminata ssp.). Viruses 13(6), 1071. Doi: https://doi.org/10.3390/v13061071
  20. Joshi, M., T.K. Narute, and M. Dawale. 2023a. Prevalence and distribution of cucumber mosaic virus disease of banana in major banana growing districts of Western Maharashtra state during 2021-2022. Pharma Innov. J. 12(9), 1808-1810.
  21. Joshi, M., T.K. Narute, and D. Sarnobat. 2023b. The cucumber mosaic virus: a review. Int. J. Plant Soil Sci. 35(24), 253-260. Doi: https://doi.org/10.9734/ijpss/2023/v35i244316
  22. Kebede, Y. and S. Majumder. 2021. Molecular characterization of Cucumber mosaic virus subgroup IB infecting Cavendish banana plants in Ethiopia. J. Plant Prot. Res. 61(4), 392-401. Doi: https://doi.org/10.24425/jppr.2021.139249
  23. Khaled-Gasmi, W., R. Souissi, and S. Boukhris-Bouhachem. 2023. Cucumber mosaic virus epidemiology in pepper: aphid dispersal, transmission efficiency and vector pressure. Ann. Appl. Biol. 182(1), 101-111. Doi: https://doi.org/10.1111/aab.12798
  24. Khan, S., A.T. Jan, B. Mandal, and Q.M.R. Haq. 2012. Immunodiagnostics of cucumber mosaic virus using antisera developed against recombinant coat protein. Arch. Phytopathol. Plant Prot. 45(5), 561-569. Doi: https://doi.org/10.1080/03235408.2011.588043
  25. Koua, D.K., A. Ebou, T.K. Kouadio, P. Lepoivre, and S. Massart. 2020. Cucumber mosaic virus and their associated satellite RNAs infecting banana (Musa sp. Genomic group AAA) in Côte d’Ivoire: a molecular characterization. BioRxiv 2020.11.17.386326. Doi: https://doi.org/10.1101/2020.11.17.386326
  26. Li, N., C. Yu, Y. Yin, S. Gao, F. Wang, C. Jiao, and M. Yao. 2020. Pepper crop improvement against cucumber mosaic virus (CMV): a review. Front. Plant Sci. 11, 598798. Doi: https://doi.org/10.3389/fpls.2020.598798
  27. Liu, Y.Y., S.L. Yu, Y.F. Lan, C.L. Zhang, S.S. Hou, X.D. Li, X.Z. Chen, and X.P. Zhu. 2009. Molecular variability of five Cucumber mosaic virus isolates from China. Acta Virol. 53(2), 89-97. Doi: https://doi.org/10.4149/av_2009_02_89
  28. López-Cardona, N., B. Villegas-Estrada, and R.E. Arango-Isaza. 2014. Evaluación de incidencia y pérdidas ocasionadas por virus que afectan cultivos de plátano y banano (Musa spp.) en la zona central cafetera. Agron. 22(1), 22-35.
  29. Manzo-Sánchez, G., M. Orozco-Santos, L. Martínez-Bolaños, E. Garrido-Ramírez, and B. Canto-Canche. 2014. Enfermedades de importancia cuarentenaria y económica del cultivo de banano (Musa sp.) en México. Rev. Mex. Fitopatol. 32(2), 89-107.
  30. Mateus-Cagua, D. and G. Rodríguez-Yzquierdo. 2019. Effect of biostimulants on dry matter accumulation and gas exchange in plantain plants (Musa AAB). Rev. Colomb. Cienc. Hortic. 13(2), 151-160. Doi: https://doi.org/10.17584/rcch.2019v13i2.8460
  31. MinAgricultura, Ministerio de Agricultura y Desarrollo Rural Colombia. 2024. Agronet: área, producción y rendimiento nacional por cultivo – plátano/plátano exportación. In: https://www.agronet.gov.co/estadistica/Paginas/home.aspx?cod=1; consulted: April, 2024.
  32. Mochizuki, T. and S.T. Ohki. 2012. Cucumber mosaic virus: viral genes as virulence determinants. Mol. Plant Pathol. 13(3), 217-225. Doi: https://doi.org/10.1111/j.1364-3703.2011.00749.x
  33. Nouri, S., R. Arevalo, B.W. Falk, and R.L. Groves. 2014. Genetic structure and molecular variability of Cucumber mosaic virus isolates in the United States. Plos One 9(5), e96582. Doi: https://doi.org/10.1371/journal.pone.0096582
  34. Palukaitis, P. and F. García-Arenal. 2003. Cucumoviruses. Adv. Virus Res. 62, 241-323. Doi: https://doi.org/10.1016/S0065-3527(03)62005-1
  35. Pérez-Vicente, L. and A. Porras. 2015. Impacto potencial del cambio climático sobre las plagas de bananos y plátanos en Cuba. Fitosanidad 19(3), 201-211.
  36. Reichel, H., S. Belalcázar, G. Múnera, E. Arévalo, and J. Narváez. 1996a. First report of Banana streak virus infecting plantains (Musa spp.) in Colombia. Plant Dis. 80(4), 463.
  37. Reichel, H., L. Mariño, J. Kummert, S. Belalcázar, and J. Narváez. 1996b. Caracterización del gen de la proteína de la cápside de dos aislamientos del virus del mosaico del pepino (CMV), obtenidos de plátano y banano (Musa spp). Corpoica Cienc. Tecnol. Agropecu. 1(1), 1-5. Doi: https://doi.org/10.21930/rcta.vol1_num1_art:145
  38. Reichel, H., A.K. Martínez, J.A. Arroyave, R. Sedano, F.J. Morales, O. Duterme, J. Kummert, and P. Lepoivre. 2003. First report of Banana mild mosaic virus isolated from plantains (Musa AAB) in Colombia. Plant Dis. 87(9), 1150. Doi: https://doi.org/10.1094/PDIS.2003.87.9.1150C
  39. Rivera-Toro, D.M., J.C. Vaca-Vaca, and K. López-López. 2020. Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops. Agron. Colomb. 38(2), 218-225. Doi: https://doi.org/10.15446/agron.colomb.v38n2.82975
  40. Roossinck, M.J. 2001. Cucumber mosaic virus, a model for RNA virus evolution. Mol. Plant Pathol. 2(2), 59-63. Doi: https://doi.org/10.1046/j.1364-3703.2001.00058.x
  41. Roossinck, M.J., L. Zhang, and K.-H. Hellwald. 1999. Rearrangements in the 5’ nontranslated region and phylogenetic analyses of Cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. J. Virol. 73(8), 6752-6758. Doi: https://doi.org/10.1128/JVI.73.8.6752-6758.1999
  42. Selvarajan, R. 2015. Viral diseases of banana. CABI Rev. 2015, 1-16. Doi: https://doi.org/10.1079/PAVSNNR201510050
  43. Stover, R.H. 1972. Banana, plantain and abaca diseases. Commonwealth Mycological Institute, Kew, UK.
  44. Tamura, K., G. Stecher, and S. Kumar. 2021. MEGA11: molecular evolutionary genetics analysis version 11. Mol. Biol. Evol. 38(7), 3022-3027. Doi: https://doi.org/10.1093/molbev/msab120
  45. Thompson, J.R., JL. Langenhan, M. Fuchs, and K.L. Perry. 2015. Genotyping of Cucumber mosaic virus isolates in western New York State during epidemic years. characterization of an emergent plant virus population. Virus Res. 210, 169-177. Doi: https://doi.org/10.1016/j.virusres.2015.07.028
  46. Vidal, E., R.K. Yokomi, A. Moreno, E. Bertolini, and M. Cambra. 2012. Calculation of diagnostic parameters of advanced serological and molecular tissue-print methods for detection of Citrus tristeza virus: a model for other plant pathogens. Phytopathology 102(1), 114-121. Doi: https://doi.org/10.1094/PHYTO-05-11-0139
  47. Viswanath, M., A. Roy, K.A. Prajwala, S.P. Nanda, B.V.K. Bhagavan, and R.R. Kumar. 2021. Virus indexing in banana: a review. Pharma Innov. J. 10(6), 1301-1304.

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