Skip to main navigation menu Skip to main content Skip to site footer

Indole butyric acid and paclobutrazol in blackberry stem cutting propagation

Blackberry fruit cv. Tupy, from the experimental plot of the UFPR. Photo: L.A. Biasi

Abstract

Blackberry is an important species among small fruits that have gained prominence in recent years in family farming in Brazil's southern and southeastern regions. Due to the lack of efficient methods in vegetative propagation, there is a need for exogenous application of growth regulators to promote root development. Therefore, the objective of this study was to evaluate the effect of indole butyric acid (IBA) and paclobutrazol (PBZ) on the rooting of stem cuttings of blackberry cv. Tupy. The cuttings were prepared with a length of 10 cm and planted in 114 cm3 tubes containing medium-sized vermiculite as substrate and conducted in a greenhouse with intermittent misting. The treatments consisted of three concentrations of IBA (500; 1,000 and 2,000 mg L-1) and PBZ (100, 200, and 400 mg mL-1) in addition to the control treatment. A completely randomized experimental design was used in a 4×4 factorial scheme, with three replicates and 10 cuttings per experimental unit. After 120 days, the percentages of rooted cuttings, callus formation, alive, dead, and sprouted cuttings, shoot length, number, length, and fresh weight of roots were evaluated. The use of plant hormones did not have a favorable effect on the propagation of blackberry cv. Tupy through stem cuttings, with inhibition of rooting observed.

Keywords

Auxin, Growth regulators, Rooting, Rubus sp.

XML PDF

References

  • Ada, R. and R. Enrico. 2020. Some urea derivatives positively affect adventitious root formation: old concepts and the state of the art. Plants 9(3), 321. Doi: https://doi.org/10.3390/plants9030321
  • Ahmed, S., S.R. Roberto, M. Shahab, R. Koyama, R.C. Colombo, I. Hussain, and Q. Sarfaraz. 2018. Improvement of blackberry rooting using mini cuttings and different methods of IBA application. Int. J. Biosci. 13(2), 1-9.
  • Andrade, R.A., A.B.G. Martins, M.T.H. Silva, and I.G. Turolla. 2007. Propagação da amora-preta por estaquia utilizando ácido indolbutírico. Rev. Caatinga 20(2), 79-83.
  • Antunes, L.E.C., N.N.J. Chalfun, and M.A. Regina. 2000. Propagação de cultivares de amoreira-preta (Rubus spp.) através de estacas lenhosas. Rev. Bras. Frutic. 22(2), 195-199.
  • Antunes, L.E.C., I.S. Pereira, L. Picolotto, G.K. Vignolo, and M.A. Gonçalves. 2014. Produção de amoreira-preta no Brasil. Rev. Bras. Frutic. 36(1), 100-111. Doi: https://doi.org/10.1590/0100-2945-450/13
  • Bitencourt, J., K.C. Zuffellato-Ribas, and H.S. Koehler. 2010. Estaquia de Ginkgo biloba L. utilizando três substratos. Rev. Bras. Plantas Med. 12(2), 135-140. Doi: https://doi.org/10.1590/S1516-05722010000200002
  • Bueno, P.M.C., M.B.D. Tofanelli, W.A. Vendrame, and L.A. Biasi. 2021. Paclobutrazol as an alternative to improve propagation of Rubus brasiliensis Mart. Sci. Hortic. 287, 110215. Doi: https://doi.org/10.1016/j.scienta.2021.110215
  • Campagnolo, M.A. and R. Pio. 2012. Enraizamento de estacas caulinares e radiculares de cultivares de amoreira-preta coletadas em diferentes épocas, armazenadas a frio e tratadas com AIB. Cienc. Rural 42(2), 232-237. Doi: https://doi.org/10.1590/S0103-84782012000200008
  • Campbell, S.M., S.L. Anderson, Z.T. Brym, and B.J. Pearson. 2021. Evaluation of substrate composition and exogenous hormone application on vegetative propagule rooting success of essential oil hemp (Cannabis sativa L.). PLoS One 16(7), e0249160. Doi: https://doi.org/10.1371/journal.pone.0249160
  • Crosa, C.F.R., R.S. Souza, T. Silveira, R.D. Marco, L.E.C. Antunes, and C.R. Martins. 2021. Propagação vegetativa de amoreira-preta das cultivares Tupy y BRS Cainguá. Res. Soc. Dev. 10(4), e23910414104. Doi: https://doi.org/10.33448/rsd-v10i4.14104
  • Debner, A.R., H. Hatterman-Valenti, and F. Takeda. 2019. Blackberry propagation limitations when using floricane cuttings. HortTechnology 29(3), 276-282. Doi: https://doi.org/10.21273/HORTTECH04266-18
  • Desta, B. and G. Amare. 2021. Paclobutrazol as a plant growth regulator. Chem. Biol. Technol. Agric. 8, 1. Doi: https://doi.org/10.1186/s40538-020-00199-z
  • Dias, P.C., G.M. Ataíde, A. Xavier, L.S. Oliveira, and H.N. Paiva. 2015. Propagação vegetativa de Schizolobium amazonicum por estaquia. CERNE 21(3), 379-386. Doi: https://doi.org/10.1590/01047760201521031467
  • Dias, J.P.T., E.O. Ono, and J. Duarte Filho. 2011. Enraizamento de estacas de brotações oriundas de estacas radiculares de amoreira-preta. Rev. Bras. Frutic. 33(1), 649-653. Doi: https://doi.org/10.1590/S0100-29452011000500090
  • Dias, J.P.T., K. Takahashi, J. Duarte Filho, and E.O. Ono. 2012. Bioestimulante na promoção da brotação em estacas de raiz de amoreira-preta. Rev. Bras. Frutic. 34(1), 1-7. Doi: https://doi.org/10.1590/S0100-29452012000100003
  • Druege, U., A. Hilo, J.M. Pérez-Pérez, Y. Klopotek, M. Acosta, F. Shahinnia, S. Zerche, P. Franken, and M.R. Hajirezaei. 2019. Molecular and physiological control of adventitious rooting in cuttings: phytohormone action meets resource allocation. Ann. Bot. 123(6), 929-949. Doi: https://doi.org/10.1093/aob/mcy234
  • Dutra, L.F., E. Kersten, and J.C. Fachinello. 2002. Época de coleta, ácido indolbutírico e triptofano no enraizamento de estacas de pessegueiro. Sci. Agric. 59(2), 327-333. Doi: https://doi.org/10.1590/S0103-90162002000200019
  • Fachinello, J.C., M.S. Pasa, J.D. Schmtiz, and D.L. Betemps. 2011. Situação e perspectivas da fruticultura de clima temperado no Brasil. Rev. Bras. Frutic. 33(Spe 1), 109-120. Doi: https://doi.org/10.1590/S0100-29452011000500014
  • Ferreira, D.F. 2011. Sisvar: a computer statistical analysis system. Cienc. Agrotec. 35(6), 1039-1042. Doi: https://doi.org/10.1590/S1413-70542011000600001
  • Gehlot, A., A. Tripathi, I.D. Arya, and S. Arya. 2015. Influence of cutting diameter, auxin and rooting substrate on adventitious rooting from hardwood cuttings of Azadirachta indica A. Juss (Neem). Adv. For. Sci. 2(3), 49-61.
  • Gilani, S.A.Q., K. Shah, I. Ahmed, A. Basit, M. Sajid, A.S. Bano, and U. Shahid. 2019. Influence of indole butyric acid (IBA) concentrations on air layerage in guava (Psidium guajava L.) cv. Sufeda. Pure Appl. Biol. 8(1), 355-362. Doi: http://doi.org/10.19045/bspab.2018.700194
  • Gomes, E. and D. Krinski. 2016. Propagação vegetativa de Piper amalago L. (Piperaceae) em função de tipos de estaca e substratos. Cult. Agron. 25(2), 199-210. Doi: https://doi.org/10.32929/2446-8355.2016v25n2p199-210
  • Han, H., S. Zhang, and X. Sun. 2009. A review on the molecular mechanism of plants rooting modulated by auxin. Afr. J. Biotechnol. 8(3), 348-353.
  • Hartmann, H.T., D.E. Kester, F.T. Davies Jr., and R.L. Geneve. 2010. Plant propagation: principles and practices. 8th ed. Prentice Hall, Upper Saddle River, NJ.
  • Hilgert, M.A., L.C. Sá, M. Lazarotto, P.V.D. Souza, and C.R. Martins. 2020. Collection period and indolebutyric acid on the rooting of adult pecan plant cuttings. Pesq. Agropecu. Bras. 55, e01656. Doi: https://doi.org/10.1590/S1678-3921.pab2020.v55.01656
  • Hussain, I., A.M. Assis, L.Y. Yamanoto, R. Koyama, and S.R. Roberto. 2014. Indole butyric acid and substrates influence on multiplication of blackberry ‘Xavante’. Cienc. Rural 44(10), 1761-1765. Doi: https://doi.org/10.1590/0103-8478cr20131204
  • Hussain, I., S.R. Roberto, R.C. Colombo, A.M. Assis, and R. Koyama. 2017. Cutting types collected at different seasons on blackberry multiplication. Rev. Bras. Frutic. 39(3), e-939. Doi: https://doi.org/10.1590/0100-29452017939
  • İşbilir, M.E., O. Saraçoğlu, E. Dinçer, A. Donat, and A.A.M. Al-Salihi. 2022. Effects of paclobutrazol applications on rooting performance of black mulberry (Morus nigra L.) hardwood cuttings. Turk. J. Agric-Food. Sci. Tech. 10(Spe 1), 2722-2724. Doi: https://doi.org/10.24925/turjaf.v10isp1.2722-2724.5682
  • Jabir, B.M.O., K.B. Kinuthia, M.A. Faroug, F. Nureldinawad, E.M. Muleke, Z. Ahmadzai, and L. Liu. 2017. Effects of gibberellin and gibberellin biosynthesis inhibitor (paclobutrazol) applications on radish (Raphanus sativus) taproot expansion and the presence of authentic hormones. Int. J. Agric. Biol. 19(4), 779-786.
  • Jamal, A., G. Ayub, A. Rahman, A. Rashid, J. Ali, and M. Shahab. 2016. Effect of IBA (Indole Butyric Acid) levels on the growth and rooting of different cutting types of Clerodendrum splendens. Pure Appl. Biol. 5(1), 64-71. Doi: http://doi.org/10.19045/bspab.2016.50009
  • Li, S.-W., L. Xue, S. Xu, H. Feng, and L. An. 2009. Mediators, genes and signaling in adventitious rooting. Bot. Rev. 75, 230-247. Doi: https://doi.org/10.1007/s12229-009-9029-9
  • Maia, A.J. and R.V. Botelho. 2008. Reguladores vegetais no enraizamento de estacas lenhosas da amoreira-preta cv. Xavante. Semina: Ciênc. Agrar. 29(2), 323-330. Doi: https://doi.org/10.5433/1679-0359.2008v29n2p323
  • Moreira, R.A., J.D. Ramos, M.C.M. Cruz, L. Villar, and O.M. Hafle. 2012. Ácido indolbutírico e polímero hidroabsorvente no enraizamento de estacas de amoreira-preta. Rev. Sci. Agrar. Parana. 11(1), 74-81. Doi: https://doi.org/10.18188/sap.v11i1.4435
  • Nasir, N.N.N.M., M.M. Khandaker, K.S. Mohd, N.A. Badaluddin, N. Osman, and N. Mat. 2021. Effect of hydrogen peroxide on plant growth, photosynthesis, leaf histology and rubisco gene expression of the Ficus deltoidea Jack Var. Deltoidea Jack. J. Plant. Growth Regul. 40, 1950-1971. Doi: https://doi.org/10.1007/s00344-020-10243-9
  • Ötvös, K., M. Marconi, A. Vega, J. O’Brien, A. Johnson, R. Abualia, L. Antonielli, J.C. Antonielli, J.C. Montesinos, Y. Zhang, S. Tan, C. Cuesta, C. Artner, E. Bouguyon, A. Gojon, J. Friml, R.A. Gutiérrez, K. Wabnik, and E. Benková. 2021. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. EMBO J. 40, e106862. Doi: https://doi.org/10.15252/embj.2020106862
  • Picolotto, L., G.K. Vignolo, I.S. Pereira, M.A. Goncalves, and L.E.C. Antunes. 2015. Enraizamento de estacas de amoreira-preta em função da adubação nitrogenada na planta matriz. Rev. Ceres. 62(3), 294-300. Doi: https://doi.org/10.1590/0034-737X201562030009
  • Pigatto, G.B, E.N. Gomes, J.C. Tomasi, A.P. Ferriani, and C. Deschamps. 2018. Effects of indolebutyric acid, stem cutting positions and substrates on the vegetative propagation of Stevia rebaudiana Bertoni. Rev. Colomb. Cienc. Hortic. 12(1), 202-211. Doi: https://doi.org/10.17584/rcch.2018v12i1.6631
  • Qadri, R., M.T. Akram, I. Khan, M. Azam, N. Nisar, M.A. Ghani, M. Tanveer, and M.M. Khan. 2018. Response of guava (Psidium guajava L.) softwood cuttings to paclobutrazol application in different rooting media. Bangladesh J. Bot. 47(3), 361-367. Doi: https://doi.org/10.3329/bjb.v47i3.38653
  • Rocha, L.C., F.A. Teixeira, M.S. Pedreira, D.D. Fries, D.L.S. Dias, E.G.L. Costa, A.J. Figuereido, A.A. Seixas, C.C. Pacheco, and B.M. Santiago. 2020. Plant growth regulator and soil fertilizer improve production and growing stage of Brachiaria decumbens. Grassl. Sci. 66(2), 102-109. Doi: https://doi.org/10.1111/grs.12260
  • Schiavon, A.V., G.L. Leivas, E.E. Delazeri, A.S. Alves, P.C. Mello-Farias, and L.E.C. Antunes. 2021. Características físico-químicas de amora-preta ‘Tupy’ colhidas em diferentes estádios de maturação e mantidas sob refrigeração. Sci. Electronic Arch. 14(8), 39-46. Doi: https://doi.org/10.36560/14820211430
  • Tadeu, M.H., R. Pio, A.S. Tiberti, M.A. Figueiredo, and F.B.M. Souza. 2012. Enraizamento de estacas caulinares e radiculares de Rubus fruticosus tratadas com AIB. Rev. Ceres 59(6), 881-884. Doi: https://doi.org/10.1590/S0034-737X2012000600021
  • Tiberti, A.S., R. Pio, C.N. Assis, K.N. Silva, and M.H. Tadeu. 2012. Propagação do 'Boysenberry' por estaquia e mergulhia. Cienc. Rural 42(3), 423-428. Doi: https://doi.org/10.1590/S0103-84782012000300007
  • Villa, F., R. Pio, N.N.J. Chalfun, T.C.A. Gontijo, and L.F. Dutra. 2003. Propagação de amoreira-preta utilizando estacas lenhosas. Cienc. Agrotec. 27(4), 829-834. Doi: https://doi.org/10.1590/S1413-70542003000400013
  • Villa, F., D.R. Stumm, D.F. Silva, F.J. Menegusso, G. Ritter, and T.R. Kohler. 2018. Rooting of Black raspberry with plant growth regulator. Cienc. Rural 48(3), e20161023. Doi: https://doi.org/10.1590/0103-8478cr20161023
  • Zheng, Y., Z. Gao, and Z. Zhu. 2016. DELLA–PIF modules: old dogs learn new tricks. Trends Plant Sci. 21(10), 813-815. Doi: https://doi.org/10.1016/j.tplants.2016.08.006

Downloads

Download data is not yet available.

Most read articles by the same author(s)

Similar Articles

1 2 3 > >> 

You may also start an advanced similarity search for this article.