Skip to main navigation menu Skip to main content Skip to site footer

Extracción, purificación y cuantificación de mangiferina en la corteza de algunos cultivares de mango (MANGIFERA INDICA L)

Abstract

La mangiferina C19H18O11 (1,3,6,7-Tetrahydroxyxantona-C2-β-D-glucósido), se aisló a partir de la corteza de 40 cultivares del árbol de Mangifera indica L, por extracción líquido/sólido y análisis cuantitativo por espectrofotometría UV-Vis. El producto se caracterizo como un polvo amorfo, amarillo verdoso, soluble en EtOH y MeOH, p.f 270 ± 2 ºC, λmax (EtOH) 370 nm. Los cultivares Pig 693 y Rosa presentan los mayores contenidos del compuesto activo con 5257,9 y 4906,2 (mg/100g bs), los de menor concentración son Bocao y Itamaraca con 698,9 y 893,8 (mg/100g bs) respectivamente. El análisis de conglomerados genero 4 grupos, donde los mangos asiáticos tuvieron un promedio superior en el contenido de mangiferina, seguidos por los estadounidenses y los suramericanos. Este trabajo es la primera investigación exploratoria que se realiza sobre esta xantona en Colombia, sustancia que presenta múltiples y efectivas aplicaciones en las áreas de fitomedicina, cosmetología y nutrición.

Keywords

Colombia, Fitoquimica, Metabolitos, Xantonas

PDF (Español)

References

  1. Beltrán, A.E., Y. Alvarez, F. E. Xavier, R. Hernanz, J. Rodriguez, A.J. Núñez, M.J. Alonso y M. Salaices. 2004. Vascular effects of the Mangifera indica L. extract (Vimang). Eur. J. Pharmacol. 499(3), 297-305. Doi: 10.1016/j.ejphar.2004.07.073
  2. Bhatia, V.K. y T.R. Seshadri. 1968. Synthesis of mangiferin. Tetrahedron Lett. 9(14), 1741-1742. Doi: 10.1016/S0040-4039(01)99040-9
  3. Chavan, J.J., D.M. Ghadage, A.S. Bhoite y S.D. Umdale. 2015. Micropropagation, molecular profiling and RPHPLC determination of mangiferin across various regeneration stages of Saptarangi (Salacia chinensis L.). Ind. Crops Prod. 76, 1123-1132. Doi: 10.1016/j.indcrop.2015.08.028
  4. Faizi, S., S. Zikr-ur-Rehman, M. Ali y A. Naz. 2006. Temperature and solvent dependent NMR studies on mangiferin and complete NMR spectral assignments of its acyl and methyl derivatives. Magn. Resonance Chem. 44(9), 838-844. Doi: 10.1002/mrc.1854
  5. Ferreira, F.d.R., I.B. Valentim, E.L.C. Ramones, M.T.S. Trevisan, C. Olea-Azar, F. Perez-Cruz, F.C. de Abreu y M.O.F. Goulart. 2013. Antioxidant activity of the mangiferin inclusion complex with β-cyclodextrin. LWT-Food Sci. Technol. 51(1), 129-134. Doi: 10.1016/j.lwt.2012.09.032
  6. Gómez-Zaleta, B., M.T. Ramírez-Silva, A. Gutiérrez, E. González-Vergara, M. Güizado-Rodríguez y A. Rojas-Hernández. 2006. UV/vis, 1H, and 13C NMR spectroscopic studies to determine mangiferin pKa values. Spectrochimica Acta Part A: Mol. Biomol.Spectrosc. 64(4), 1002-1009. Doi: 10.1016/j.saa.2005.09.009
  7. Han, D., C. Chen, C. Zhang, Y. Zhang y X. Tang. 2010. Determination of mangiferin in rat plasma by liquid-liquid extraction with UPLC-MS/MS. J. Pharm. Biomed. Anal. 51(1), 260-263. Doi: 10.1016/j.jpba.2009.07.021
  8. Joubert, E., M. Botha, C. Maicu, D. De Beer y M. Manley. 2012. Rapid screening methods for estimation of mangiferin and xanthone contents of Cyclopia subternata plant material. South Afr. J. Bot. 82, 113-122. Doi: 10.1016/j.sajb.2012.07.019
  9. Kawakami, C.M. y L.R. Gaspar. 2015. Mangiferin and naringenin affect the photostability and phototoxicity of sunscreens containing avobenzone. J. Photochem. Photobiol. B: Biol. 151, 239-247. Doi: 10.1016/j.jphotobiol.2015.08.014
  10. Kulkarni, V.M. y V.K. Rathod. 2014. Extraction of mangiferin from Mangifera indica leaves using three phase partitioning coupled with ultrasound. Ind. Crops Prod. 52, 292-297. Doi: 10.1016/j.indcrop.2013.10.032
  11. Kulkarni, V.M. y V.K. Rathod. 2015. A novel method to augment extraction of mangiferin by application of microwave on three phase partitioning. Biotechnol. Rep. 6, 8-12. Doi: 10.1016/j.btre.2014.12.009
  12. Leiro, J.M., E. Álvarez, J.A. Arranz, I.G. Siso y F. Orallo. 2003. In vitro effects of mangiferin on superoxide concentrations and expression of the inducible nitric oxide synthase, tumour necrosis factor-β and transforming growth factor-β genes. Biochem. Pharm. 65(8), 1361-1371. Doi: 10.1016/S0006-2952(03)00041-8
  13. Martínez, G., R. Delgado, G. Pérez, G. Garrido, A.J. Núñez S. y O.S. León. 2000. Evaluation of the in vitro antioxidant activity of Mangifera indica L. extract (Vimang). Phytother. Res. 14(6), 424-427. Doi: 10.1002/1099-1573(200009)14:6<424::AID-PTR643>3.0.CO;2-8
  14. Mishra, B., K.I. Priyadarsini, M. Sudheerkumar, M.K. Unnikrishhnan y H. Mohan. 2006. Pulse radiolysis studies of mangiferin: A C-glycosyl xanthone isolated from Mangifera indica. Rad. Phys. Chem. 75(1), 70-77. Doi: 10.1016/j.radphyschem.2005.05.015
  15. Montes, A., L. Wehner, C. Pereyra y E.J.M. Ossa. 2016. Mangiferin nanoparticles precipitation by supercritical antisolvent process. J. Supercrit. Fluids 112, 44-50. Doi: 10.1016/j.supflu.2016.02.008
  16. Núñez S., A.J., H.T. Vélez C., J. Agüero-Agüero, J. González-González, F. Naddeo, F. De Simone y L. Rastrelli. 2002. Isolation and quantitative analysis of phenolic antioxidants, free sugars, and polyols from mango (Mangifera indica L.) stem bark aqueous decoction used in Cuba as a nutritional supplement. J. Agric. Food Chem. 50(4), 762-766. Doi: 10.1021/jf011064b
  17. Qiu, X., J.-l. Zhao, C. Hao, C. Yuan, N. Tian, Z.-S. Xu y R.- M. Zou. 2016. Simultaneous determination of mangiferin and neomangiferin in rat plasma by UPLC-MS/ MS and its application for pharmacokinetic study. J. Pharm. Biomed. Anal. 124, 138-142. Doi: 10.1016/j.jpba.2016.02.034
  18. Ribeiro, S.M.R., L.C.A. Barbosa, J.H. Queiroz, M. Knödler y A. Schieber. 2008. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chem. 110(3), 620-626. Doi: 10.1016/j.foodchem.2008.02.067
  19. Ruiz-Montañez, G., J.A. Ragazzo-Sánchez, M. Calderón-Santoyo, G. Velázquez-de la Cruz, J.A. Ramírez de León y A. Navarro-Ocaña. 2014. Evaluation of extraction methods for preparative scale obtention of mangiferin and lupeol from mango peels (Mangifera indica L.). Food Chem. 159, 267-272. Doi: 10.1016/j.foodchem.2014.03.009
  20. Scartezzini, P. y E. Speroni. 2000. Review on some plants of Indian traditional medicine with antioxidant activity. J. Ethnopharm. 71(1-2), 23-43. Doi: 10.1016/S0378-8741(00)00213-0
  21. Sethiya, N.K., A. Trivedi y S.H. Mishra. 2015. Rapid validated high performance thin layer chromatography method for simultaneous estimation of mangiferin and scopoletin in Canscora decussata (South Indian Shankhpushpi) extract. Rev. Bras. Farmacognosia 25(3), 193-198. Doi: 10.1016/j.bjp.2015.04.002

Downloads

Download data is not yet available.

Similar Articles

1 2 3 4 > >> 

You may also start an advanced similarity search for this article.