LED lights in the <i>in vitro</i> multiplication of three banana cultivars
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Gomes da Rocha, P. S., Pedroso de Oliveira, R., Bueno Scivittaro, W., & Mosele, S. (2018). LED lights in the <i>in vitro</i> multiplication of three banana cultivars. Revista Colombiana De Ciencias Hortícolas, 11(2), 247-252. https://doi.org/10.17584/rcch.2017v11i2.6666
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Autores
Paulo Sérgio Gomes da Rocha
http://orcid.org/0000-0001-5275-1621
Roberto Pedroso de Oliveira
http://orcid.org/0000-0002-5402-6834
Walkyria Bueno Scivittaro
http://orcid.org/0000-0002-6709-6831
Sergio Henrique Mosele
http://orcid.org/0000-0001-5878-8097
Abstract
Light emitting diodes (LEDs) are considered the most promising new source of light for the in vitro cultivation of plants because they have a long list of benefits and positively affect the growth of explants. The objective of this study was to evaluate the use of LEDs as an alternative light source, using fluorescent lamps in the multiplication of three banana cultivars. The Grande Naine, FHIA 18 and Prata Anã cultivars were placed in an MS (Murashige and Skoog) medium with 30 g L-1 sucrose, 100 mg L-1 myo-inositol, 7 g L-1 agar and 6 mg L-1 BAP; three successive subcultures were carried out for 30 days under five light sources (blue-EDEB LEDs 3LA1 470 nm, green-TEDS LEDs 3LA1 530 nm, red-EDER LEDs 3LA3 630 nm, Growlux fluorescent lamps and white fluorescent lamps), with a 16 hour photoperiod, temperature of 25±2°C and light intensity of 20 µmol m-2 s-1. The design was completely randomized with a 5×3 factorial (light source × cultivar), with five repetitions. For all three cultivars, it was observed that the number of shoots and leaves was not influenced by the type of light. The highest number of formed shoots was obtained with the cultivar Grande Naine (2.6 buds/explant), with no difference between cv. FHIA 18 and Prata Anã (1.4 and 1.3 shoots/explant). There was a significant difference between the cultivars and light source for the length of budding; the longest length was obtained with Prata Anã, grown under green LEDs.
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References
Bello, J.J.B., E.M. Estrada, J.H.C. Velázquez e E.V.M. Ramos. 2016. Effect of LED light quality on in vitroshoot roliferation and growth of vanilla (Vanilla planifólia Andrews). Afr. J. Biotec. 15(8), 272-277. Doi: https://doi.org/10.5897/AJB2015.14662
Camolesi, M.R., A.N. Martins, L.D. Souza e C.G. Saconi. 2010. Enraizamento in vitro de mudas micropropagadas de bananeira (Musa sp.) em diferentes meios de cultivo. Ciênc Agrotec. 34(6), 1446-1451. Doi: https://doi.org/10.1590/S1413-70542010000600013
Carvalho, A.C.P., A.A.J. Rodrigues e E.O. Santos. 2012. Produção de mudas micropropagadas de bananeira. Em: Circular Técnica 37, http://ainfo.cnptia.embrapa.br/digital/bitstream/item/72455/1/CIT12001.pdf; consulta: fevereiro de 2017.
Costa, F.H.S., J.E.S. Pereira, M.A.A. Pereira e J.P. Oliveira. 2006. Efeito da interação entre carvão ativado e N6-benzilaminopurina na propagação in vitro de bananeira, cv. Grand Naine. Rev. Bras. Frutic. 28(2), 280-283. Doi: https://doi.org/10.1590/S0100-29452006000200028
FAO. 2017. Production. Em: http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567; consulta: fevereiro de 2017.
Ferreira, L.T., M.M.A. Silva, C. Ulisses, T. Camara e L. Willadino. 2017. Using LED lighting in somatic embryogenesis and micropropagation of an elite surgacane variety and its effect on redox metabolism during acclimatization. Plant Cell Tiss. Organ Cult. 128, 211-221. Doi: https://doi.org/10.1007/s11240-016-1101-7
Gupta, S.D. e B. Jatothu. 2013. Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotechn. Rep. 7(3), 211-220. Doi: https://doi.org/10.1007/s11816-013-0277-0
Heo, J.W., K.S. Shin, S.K. Kim e K.Y. Paek. 2006. Light quality affects in vitro growth of grape ‘Teleki 5BB’. J. Plant Biol. 49, 276-280. Doi: https://doi.org/10.1007/BF03031155
Kim, S.J., E.J. Hahn, J.W. Heo e K.Y. Paek. 2004. Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci. Hortic. 110, 143-151. Doi: https://doi.org/10.1016/j.scienta.2003.10.003
Medeiros, D.S. 2015. Taxa de multiplicação de mudas micropropagadas de bananeira cv. Grande naine e cv. Prata catarina influenciada pela fase de estabelecimento de cultura. Dissertação (mestrado). Universidade Federal de Santa Catarina, Florianópolis, Brasil.
Moon, H.K., S.Y. Park, Y.W. Kim e C.S. Kim. 2006. Growth of Tsuru-rindo (Tripterospermum japonicum) cultures in vitro under various sources of light-emitting diode (LED) irradiation. J. Plant Biol. 49(2), 174-179. Doi: https://doi.org/10.1007/BF03031014
Murashige, T. e F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497. Doi: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nhut, D.T., T. Takamura, H. Watanabe e M.M. Tanaka. 2003. Efficiency of a novel culture system by using light-emitting diode (LED) on in vitro and subsequent growth of micropropagated banana plantlets. Acta Hortic. 616, 121-127. Doi: https://doi.org/10.17660/ActaHortic.2003.616.10
Oliveira, H.S., O.F. Lemos, V.S. Miranda, H.C.P. Moura, M.F. Campelo e L.R.R. Santos. 2011. Estabelecimento e multiplicação in vitro de brotos no processo de micropropagação de cultivares de bananeira (Musa spp.). Acta Amaz. 41, 369-376. Doi: https://doi.org/10.1590/S0044-59672011000300006
Oliveira, R.P. e S.O. Silva. 1997. Avaliação da micropropagação comercial de bananeira. Pesq. Agrop. Bras. 32, 415-420.
Poudel, P.R., I. Kataoka e R. Mochioka. 2008. Effect of red and blue light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss. Organ Cult. 92(2), 147-153. Doi: https://doi.org/10.1007/s11240-007-9317-1
Rocha, H.S., C.R.R. Silva, A.G. Araújo e A.B. Silva. 2007. Propagação in vitro de bananeira ‘Prata Anã’: intensidade luminosa e concentrações de sacarose nas fases de multiplicação e enraizamento. Plant Cell Cult. Microprop. 3, 10-16.
Rocha, P.S.G., R.P. Oliveira, W.B. Scivittaro e U.L. Santos. 2010. Diodos emissores de luz e concentrações de BAP na multiplicação in vitro de morangueiro. Ciênc. Rur. 40(9), 1922-1928. Doi: https://doi.org/10.1590/S0103-84782010000900011
Rocha, P.S.G., R.P. Oliveira e W.B. Scivittaro. 2013a. Diodos emissores de luz e concentrações (LEDs) na micropropagação de amoreira-preta cv. Tupy. Hortic. Argent. 32(79), 14-19.
Rocha, P.S.G., R.P. Oliveira e W.B. Scivittaro. 2013b. Sugarcane micropropagation using light emitting diodes and ajustment in growty-medium sucrose concentration. Ciênc. Rur. 43(7), 1168-1173. Doi: https://doi.org/10.1590/S0103-84782013000700005
Rocha, P.S.G., R.P. Oliveira e W.B. Scivittaro. 2015. New light sources for in vitro potato micropropagation. Biosci. J. 31(5), 1312-1318. Doi: https://doi.org/10.14393/BJ-v31n5a2015-26601
Yeh, N. e J.P. Chung. 2009. High-brightness LEDs: energy efficient lighting sources and their potential in door plant cultivation. Renew Sust Energ Rev. 13, 2175-2180. Doi: https://doi.org/10.1016/j.rser.2009.01.027
Wozny, A. e N. Miler. 2016. LEDs application in ex vitro rooting and acclimatization of crysanthemum (Chrysanthemum x Grasndiflorum/Ramat./Kitam). Elet. J. Pol. Agric. Univer. 19(4), 1-8.
Camolesi, M.R., A.N. Martins, L.D. Souza e C.G. Saconi. 2010. Enraizamento in vitro de mudas micropropagadas de bananeira (Musa sp.) em diferentes meios de cultivo. Ciênc Agrotec. 34(6), 1446-1451. Doi: https://doi.org/10.1590/S1413-70542010000600013
Carvalho, A.C.P., A.A.J. Rodrigues e E.O. Santos. 2012. Produção de mudas micropropagadas de bananeira. Em: Circular Técnica 37, http://ainfo.cnptia.embrapa.br/digital/bitstream/item/72455/1/CIT12001.pdf; consulta: fevereiro de 2017.
Costa, F.H.S., J.E.S. Pereira, M.A.A. Pereira e J.P. Oliveira. 2006. Efeito da interação entre carvão ativado e N6-benzilaminopurina na propagação in vitro de bananeira, cv. Grand Naine. Rev. Bras. Frutic. 28(2), 280-283. Doi: https://doi.org/10.1590/S0100-29452006000200028
FAO. 2017. Production. Em: http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567; consulta: fevereiro de 2017.
Ferreira, L.T., M.M.A. Silva, C. Ulisses, T. Camara e L. Willadino. 2017. Using LED lighting in somatic embryogenesis and micropropagation of an elite surgacane variety and its effect on redox metabolism during acclimatization. Plant Cell Tiss. Organ Cult. 128, 211-221. Doi: https://doi.org/10.1007/s11240-016-1101-7
Gupta, S.D. e B. Jatothu. 2013. Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotechn. Rep. 7(3), 211-220. Doi: https://doi.org/10.1007/s11816-013-0277-0
Heo, J.W., K.S. Shin, S.K. Kim e K.Y. Paek. 2006. Light quality affects in vitro growth of grape ‘Teleki 5BB’. J. Plant Biol. 49, 276-280. Doi: https://doi.org/10.1007/BF03031155
Kim, S.J., E.J. Hahn, J.W. Heo e K.Y. Paek. 2004. Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci. Hortic. 110, 143-151. Doi: https://doi.org/10.1016/j.scienta.2003.10.003
Medeiros, D.S. 2015. Taxa de multiplicação de mudas micropropagadas de bananeira cv. Grande naine e cv. Prata catarina influenciada pela fase de estabelecimento de cultura. Dissertação (mestrado). Universidade Federal de Santa Catarina, Florianópolis, Brasil.
Moon, H.K., S.Y. Park, Y.W. Kim e C.S. Kim. 2006. Growth of Tsuru-rindo (Tripterospermum japonicum) cultures in vitro under various sources of light-emitting diode (LED) irradiation. J. Plant Biol. 49(2), 174-179. Doi: https://doi.org/10.1007/BF03031014
Murashige, T. e F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497. Doi: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nhut, D.T., T. Takamura, H. Watanabe e M.M. Tanaka. 2003. Efficiency of a novel culture system by using light-emitting diode (LED) on in vitro and subsequent growth of micropropagated banana plantlets. Acta Hortic. 616, 121-127. Doi: https://doi.org/10.17660/ActaHortic.2003.616.10
Oliveira, H.S., O.F. Lemos, V.S. Miranda, H.C.P. Moura, M.F. Campelo e L.R.R. Santos. 2011. Estabelecimento e multiplicação in vitro de brotos no processo de micropropagação de cultivares de bananeira (Musa spp.). Acta Amaz. 41, 369-376. Doi: https://doi.org/10.1590/S0044-59672011000300006
Oliveira, R.P. e S.O. Silva. 1997. Avaliação da micropropagação comercial de bananeira. Pesq. Agrop. Bras. 32, 415-420.
Poudel, P.R., I. Kataoka e R. Mochioka. 2008. Effect of red and blue light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss. Organ Cult. 92(2), 147-153. Doi: https://doi.org/10.1007/s11240-007-9317-1
Rocha, H.S., C.R.R. Silva, A.G. Araújo e A.B. Silva. 2007. Propagação in vitro de bananeira ‘Prata Anã’: intensidade luminosa e concentrações de sacarose nas fases de multiplicação e enraizamento. Plant Cell Cult. Microprop. 3, 10-16.
Rocha, P.S.G., R.P. Oliveira, W.B. Scivittaro e U.L. Santos. 2010. Diodos emissores de luz e concentrações de BAP na multiplicação in vitro de morangueiro. Ciênc. Rur. 40(9), 1922-1928. Doi: https://doi.org/10.1590/S0103-84782010000900011
Rocha, P.S.G., R.P. Oliveira e W.B. Scivittaro. 2013a. Diodos emissores de luz e concentrações (LEDs) na micropropagação de amoreira-preta cv. Tupy. Hortic. Argent. 32(79), 14-19.
Rocha, P.S.G., R.P. Oliveira e W.B. Scivittaro. 2013b. Sugarcane micropropagation using light emitting diodes and ajustment in growty-medium sucrose concentration. Ciênc. Rur. 43(7), 1168-1173. Doi: https://doi.org/10.1590/S0103-84782013000700005
Rocha, P.S.G., R.P. Oliveira e W.B. Scivittaro. 2015. New light sources for in vitro potato micropropagation. Biosci. J. 31(5), 1312-1318. Doi: https://doi.org/10.14393/BJ-v31n5a2015-26601
Yeh, N. e J.P. Chung. 2009. High-brightness LEDs: energy efficient lighting sources and their potential in door plant cultivation. Renew Sust Energ Rev. 13, 2175-2180. Doi: https://doi.org/10.1016/j.rser.2009.01.027
Wozny, A. e N. Miler. 2016. LEDs application in ex vitro rooting and acclimatization of crysanthemum (Chrysanthemum x Grasndiflorum/Ramat./Kitam). Elet. J. Pol. Agric. Univer. 19(4), 1-8.
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