Effects of corm size and benzylaminopurine on plantain proliferation in two propagation environments
- Jorge Espinoza González
, - Antonio Bustamante González,
- Galo Cedeño García
Abstract
Macropropagation of plantain in a thermal chamber is an economic technology, effective as a method of phytosanitary cleaning, and efficient to enhance the production of seedlings. The aim of the research was to evaluate the effects of corm size (CZ) and benzylaminopurine (BAP) on the proliferation of plantain seedlings cv. Barraganete in two propagation environments (PE). The treatments consisted of two levels of BAP (with and without BAP), three CZ (2±0.5, 4±0.5 and 6±0.5 kg) and two PE (thermal chamber and raised bed). The variables evaluated were budding time (days), multiplication rate (MR) per unit (seedlings per corm) and per area (seedlings per m2). The budding time was significantly influenced (p < 0.05) by the PE, where the thermal chamber advanced the emergence of shoots by 12 days, with respect to the raised bed. The MR of seedlings per corm and m2 were significantly influenced (p < 0.05) by BAP x PE and CZ x PE interactions, where the highest production of seedlings per corm occurred within the thermal chamber with BAP and corms of 6±0.5 kg, while the production of seedlings per m2 was higher with 2±0.5 kg corms under the same thermal chamber conditions and with BAP. The main effects results reported that with BAP there was a 30 and 31% increase in MR per corm and per m2, respectively, in relation to the treatment without BAP. Within the thermal chamber, the MR per corm and per m2 increased by 44% with respect to the raised bed. Regarding the effect of CZ, larger corms reached a higher individual MR, while those of smaller size reached a higher MR per area. It is recommended to use a thermal chamber and BAP to massify the production of plantain seedlings via macropropagation.
Keywords
Musa AAB, Macropropagación, Rizoma, Cámara térmica, Fitorregulador
References
- Álvarez, E., G. Ceballos, L. Gañán, D. Rodríguez, S. González y A. Pantoja. (2013). Producción de material de "siembra" limpio en el manejo de las enfermedades limitantes del plátano. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. 16 p.
- Ashango, T. (2017). Effect of corm and corm pieces on regeneration and multiplication of enset (Ensete ventricosum (Welw.) Cheesman). International Journal of Research – GRANTHAALAYAH, 5(5), 281-289. https://doi.org/10.29121/granthaalayah.v5.i5.2017.1860. DOI: https://doi.org/10.29121/granthaalayah.v5.i5.2017.1860
- Beltrón C., A. Sánchez y M. Ortiz. (2018). El fortalecimiento de la comercialización del plátano mediante formas asociativas. caso de estudio del cantón El Carmen de la provincia de Manabí. Revista Caribeña de Ciencias Sociales (agosto 2018). Recuperado en línea: https://www.eumed.net/rev/caribe/2018/08/comercializacion-platano-ecuador.html
- Bangata, B., K. Mobambo, M. Kasongo, D. Shungu, K. Vuvu, P. Vangu. et al. (2018). Évaluation du potentiel prolifératif de six cultivars de bananier (cv. AAB, ABB, et AAA) par macropropagation en République Démocratique du Congo. Journal of Applied Biosciences, 127(1), 12770-12784. https://doi.org/10.4314/jab.v127i1.3. DOI: https://doi.org/10.4314/jab.v127i1.3
- Baganta, B. M., N. Ngbenelo et K. Mobambo. (2019). Evaluation du potentiel de prolifération d’explants de différentes dimensions de bananier plantain (Musa sp. cv. AAB) par la macropropagation en conditions semi-contrôlées. Revue Africaine d’Environnement et d’Agriculture, 2(2), 25-31.
- Cedeño, G. A., H. Soplín, S. Helfgott, G. Cedeño y I. Sotomayor. (2016). Aplicación de biorreguladores para la macro- propagación del banano cv. Williams en cámara térmica. Agron Mesoam, 27(2), 397-408. http://dx.doi.org/10.15517/am.v27i2.24390 DOI: https://doi.org/10.15517/am.v27i2.24390
- Cheng, Z., Wang, L., Sun, W., Zhang, Y., Zhou, C., Su, Y., Li, W., Sun, T., Zhao, X., Li, X., Cheng, Y., Zhao, Y., Xie, Q., and Zhang, X. (2013). Pattern of Auxin and Cytokinin Responses for Shoot Meristem Induction Results from the Regulation of Cytokinin Biosynthesis by AUXIN RESPONSE FACTOR3. Plant Physiology, 161(1), 240-251. https://doi.org/10.1104/pp.112.203166 DOI: https://doi.org/10.1104/pp.112.203166
- Dhaval, P., and Keara, F. (2009). Temperature-regulation of plant architecture. Plant Signaling & Behavior, 4(7), 577-579. https://doi:10.4161/psb.4.7.8849. DOI: https://doi.org/10.4161/psb.4.7.8849
- Deo, B., Keshari, B and Pradhan, B. (2019). In vitro propagation of popular banana cultivar (Musa spp. Cv. Patakpura). Bangladesh Journal of Agricultural Research, 44(4), 641-648. https://doi.org/10.3329/bjar.v44i4.45699 DOI: https://doi.org/10.3329/bjar.v44i4.45699
- Galán, V., Rangel, A., Lopez, J., Perez, J., Sandoval, J., y Souza, H. (2018). Propagación del banano: técnicas tradicionales, nuevas tecnologías e innovaciones. Rev. Bras. Frutic., Jaboticabal, 40, 4, e-574. https://doi.org/10.1590/0100-29452018574 DOI: https://doi.org/10.1590/0100-29452018574
- Hatfield, J., and Prueger, J. (2015). Temperature extremes: Effect on plant growth and development. Weather and Climate Extremes, 10, 4–10. https://doi.org/10.1016/j.wace.2015.08.001. DOI: https://doi.org/10.1016/j.wace.2015.08.001
- Jacobsen, K., Omondi, B., Almekinder, C., Alvarez, E., Blomme, G., Dita, M., Iskra-Caruana, M., Ocimatii, W., Tinzaara, W., Kumar, P., and Staver, C. (2019). Seed degeneration of banana planting materials: strategies for improved farmer access to healthy seed. Plant Pathology, 68(1), 207-228. https://doi.org/10.1111/ppa.12958 DOI: https://doi.org/10.1111/ppa.12958
- Coyne, D., A. Wasukira, J. Dusabe, I. Rotifa and T. Dubois. (2010). Boiling water treatment: A simple, rapid and effective technique for nematode and banana weevil management in banana and plantain (Musa spp.) planting material. Crop Protection, 29(12), 1478-1482. https://doi.org/10.1016/j.cropro.2010.08.008 DOI: https://doi.org/10.1016/j.cropro.2010.08.008
- Koné, T., Koné, M., Teixeira, J., Koné, D., and Kouadio, J. (2011). Effect of substrate type and bulb size on in vivo production of seedlings in three cultivars of plantain (Musa spp.). The African Journal of Plant Science and Biotechnology, 5(1), 50-55.
- Koné, T., Soumahoro, A., Coulibaly, Z., Traore, S., Koné, D., and Koné, M. (2016). Effects of substrates, weight and physiological stage of suckers on massive propagation of plantain (Musa paradisiaca L.). International Journal of Research – GRANTHAALAYAH, 4(1), 1-13. https://doi.org/10.29121/granthaalayah.v4.i1.2016.2837. DOI: https://doi.org/10.29121/granthaalayah.v4.i1.2016.2837
- Lassois L., Lepoivre P., Swennen R., van den Houwe I., and Panis B. (2012) Thermotherapy, Chemotherapy, and Meristem Culture in Banana. In: Lambardi M., Ozudogru E., Jain S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology (Methods and Protocols), vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_32 DOI: https://doi.org/10.1007/978-1-62703-074-8_32
- López, J., Cedeño, G., y Cedeño, G.A. (2021). Efectos de bencilaminopurina y tipo de brotes en la producción y calidad de plántulas de plátano vía macropropagación. Revista ALFA, 5(15), 386-399. https://doi.org/10.33996/revistaalfa.v5i15.124 DOI: https://doi.org/10.33996/revistaalfa.v5i15.124
- MAG (Ministerio de Agricultura y Ganadería). (2020). Sistema de Información Pública Agropecuaria, Ficha técnica del cultivo de plátano (Musa AAB). Recuperado en línea: https://n9.cl/5d6fp
- Njau, N., Mwangi, M., Gathu, R., Mbaka, J., and Muasya, R. (2011). Banana weevil (Cosmopolites sordidus) reduces availability of corms for seedling production through macropropagation technology. Journal of Animal & Plant Sciences, 12(1), 1537-1542.
- Ntamwira, J., Sivirihauma, C., Ocimati, W., Bumba, M., Vutseme, L., Kamira, M., and Blomme, G. (2017). Macropropagation of banana/plantain using selected local materials: A cost-effective way of mass propagation of planting materials for resource-poor households. European Journal of Horticultural Science, 82(1), 38-53. https://doi.org/10.17660/eJHS.2017/82.1.5 DOI: https://doi.org/10.17660/eJHS.2017/82.1.5
- Opata, J., Melichar, P., Hegele, M., Hegele, S., Dzomeku, B., and Wünsche, J. (2020). Macropropagation of plantain (Musa AAB): Responses to hormonal and mechanical corm manipulation. Fruits, 75(3), 123-129. https://doi.org/10.17660/th2020/75.2.3 DOI: https://doi.org/10.17660/th2020/75.2.3
- Panattoni, A., Luvisi, A., and Triolo, E. (2013). Review. Elimination of viruses in plants: twenty years of progress. Spanish Journal of Agricultural Research, 11(1), 173–188. https://doi.org/10.5424/sjar/2013111-3201 DOI: https://doi.org/10.5424/sjar/2013111-3201
- Pereira, G., Santaella, M., Alves, L., Silva, E., Flenga, A., and Santos, D. (2019). Concentrations of 6-Benzylaminopurine (BAP) in micropropagation of banana ‘Farta Velhaco’ (AAB). Comunicata Scientiae, 9(1):58-63. https://doi.org/10.14295/CS.v9i1.2034 DOI: https://doi.org/10.14295/cs.v9i1.2034
- Ramirez, J., Batoom, G., and Sacayanan, A. (2016). Macropropagation of Saba Banana Using Misting System and Different Plant Growth Enhancers. MAYFEB Journal of Agricultural Science, 4, 28–33.
- Rodríguez, D., Ceballos, G., Mejía, J., Álvarez, E., y Lugo, O. (2013). Construcción, implementación y estandarización de cámara térmica para producción de semilla de plátano libre enfermedades. Centro Internacional de Agricultura Tropical (CIAT), Cali, COL.
- Su, Y., and Zhang, X. (2014). The Hormonal Control of Regeneration in Plants. Current Topics in Developmental Biology, 108, 35-69. https://doi.org/10.1016/B978-0-12-391498-9.00010-3 DOI: https://doi.org/10.1016/B978-0-12-391498-9.00010-3
- Smith, R. (2013) Plant Tissue Culture (Third Edition). Chapter 4, Pages 45-51 - Explant Preparation. Academic Press Elsevier, San Diego. https://doi.org/10.1016/B978-0-12-415920-4.00004-9 DOI: https://doi.org/10.1016/B978-0-12-415920-4.00004-9
- Shi, W., Villar-Salvador, P., Li, G., and Jiang, X. (2019). Acorn size is more important than nursery fertilization for outplanting performance of Quercus variabilis container seedlings. Annals of Forest Science, 76, 22. https://doi.org/10.1007/s13595-018-0785-8 DOI: https://doi.org/10.1007/s13595-018-0785-8
- Shin, J., Bae, S., and Seo, P. (2020). De novo shoot organogenesis during plant regeneration. Review paper. Journal of Experimental Botany, 71(1), 63-72. https://doi.org/10.1093/jxb/erz395 DOI: https://doi.org/10.1093/jxb/erz395
- Taji, A., and Williams, R. (2005). Use of in vitro breeding strategies in the development of Australian native plants. Acta Horticulturae, 683, 87-93. https://doi.org/10.17660/ActaHortic.2005.683.7 DOI: https://doi.org/10.17660/ActaHortic.2005.683.7
- Thiemele, D., Issali, A., Traore, S., Kouassi, K., Aby, N., Gnonhouri, P., Kobenan, J., Yao, T., Adiko, A., and Zakra, A. (2015). Macropropagation of plantain (Musa spp.) Cultivars PITA 3, FHIA 21, ORISHELE and CORNE 1: effect of benzylaminopurine (BAP) concentration. Journal of Plant Development, 22, 31-39.
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