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Vol. 13 No. 1 (2016)

PAPERS

Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique

https://doi.org/10.19053/01228420.4810

Published 2016-01-01

  • Rosa Isabel Higuera-Piedrahita
    • ,
  • María Eugenia López-Arellano
    • ,
  • Raquel López-Arellano
    • ,
  • César Cuenca-Verde
    • ,
  • Jorge Alfredo Cuéllar-Ordaz

Rosa Isabel Higuera-Piedrahita
Universidad Nacional Autónoma de México (Cuautitlán Izcalli, México).

María Eugenia López-Arellano
Instituto Nacional de Investigaciones Forestales (Cuernavaca, México).

Raquel López-Arellano
Universidad Nacional Autónoma de México (Cuautitlán Izcalli, México).

César Cuenca-Verde
Universidad Nacional Autónoma de México (Cuautitlán Izcalli, México).

Jorge Alfredo Cuéllar-Ordaz
Universidad Nacional Autónoma de México (Cuautitlán Izcalli, México).

Abstract

The new approach of integrated nematode control has driven to look for other alternatives that consider steadily and sustainable the environment. The aim of the present study was to obtain different ethanolic fracctions from Artemisia cina plant, using artemisinins, and to evaluate the nematicide association capacity effect with infective larvae (L3) and abomasal explant. The artemisinins from A. cina were obtained by thin layer chromatography and were evaluated on unsheathed L3 of Haemonchus contortus. The abomasal technique explants were performed for triplicate and compared as follows: three control with levamisole (7.5 mg/ml), commercial artemisinin (1 mg/ml), and distillate water, and six A. cina fracctions at 1 mg/ml each one: Ac3k, Ac3b, Ac3a, Ac3h, Ac3i and Ac4b. No significant differences between fractions of A. cina and commercial artemisinin (p>0.05) were obtained. These findings led us to conclude that there was no association capacity effect of fractions of A. cina on unsheathed L3 of H. contortus, to the abomasal tissue. It is important to continue with more studies to determine the parasitic phase in which the artemisinin viability is susceptible to be affected.

Keywords

abomasal explants, artemisia cina, aitemisinin, ethanolic extract, haemonchus contortus.

PDF (Español)

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