Skip to main navigation menu Skip to main content Skip to site footer

Vol. 18 No. 1 (2024)

Section on aromatic, medicinal and spice plants

Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species

https://doi.org/10.17584/rcch.2024v18i1.16885

Published 2024-01-01

  • Doris Gutiérrez

Doris Gutiérrez
Jardín Botánico de Bogotá José Celestino Mutis, Subdirección Científica, Bogota (Colombia)

Peperomia subspathulata Yunk. Agroecological farm, Jardín Botánico José Celestino Mutis. Photo: D. Gutiérrez

Abstract

Peperomia subspathulata Yunck is an aromatic plant of the Piperaceae family. This species is native to Colombia and Ecuador. It is used in food preparation for its aroma and has been traditionally used to treat blows and wounds. The present study evaluated the essential oil and the ethanolic extract of aerial parts from P. subspathulata against Aspergillus sp., Botrytis sp., and Penicillium sp. The essential oil was obtained by hydrodistillation, and gas chromatography coupled with mass spectrometry analyzed its composition. The ethanolic extract was obtained by maceration with ethanol 96%. The antifungal activity tests were conducted in a potato dextrose agar medium which different concentration of essential oil and extract were added. The main components identified in the essential oil were safrole (44.3%), α-bisabolol (24.2%), myristicin (4.7%), trans-β-caryophyllene (3.0%), viridiflorene (30%), α-humulene (2.3%), trans-nerolidol (1.5%), linalool (1.1%), methyleugenol (1.1%) and cis-farnesene (1.0%). The total phenolic content of the ethanolic extract was determined by the Folin-Ciocalteu method (48.5±0.5 mg gallic acid equivalent GAE/g of extract). The ethanolic extract and the essential oil inhibited the growth of microorganisms. The essential oil was the most effective against Botrytis sp. (MIC=500 µg mL-1) and Aspergillus (MIC=1,000 µg mL-1).

Keywords

Phytopathogenic fungi, Essential oil, Ethanolic extract, Medicinal plants, Bio-assays

XML PDF

References

  1. Akinnibosun, H.A., F.I. Akinnibosun, and B.E. German. 2008. Antibacterial activity of aqueous and ethanolic leaf extracts of Peperomia pellucida (l.) HB & K.(Piperaceae) on three gram-negative bacteria isolates. Sci. World J. 3(4), 33-36. Doi: https://doi.org/10.4314/swj.v3i4.51825
  2. Al Aboody, M.S. and S. Mickymaray. 2020. Anti-fungal efficacy and mechanisms of flavonoids. Antibiotics 9(2), 45. Doi: https://doi.org/10.3390/antibiotics9020045
  3. Albarracin, L.T., W.A. Delgado, L.E. Cuca, and M.C. Ávila. 2017. Chemical constituents of the bark of Endlicheria oreocola (Lauraceae) from Colombia. Biochem. Syst. Ecol. 74, 60-62. Doi: https://doi.org/10.1016/j.bse.2017.09.003
  4. Al-Madhagi, W.M., N.M. Hashim, N.A. Ali, and R. Othman. 2019. Phytochemical screening, cytotoxic and antimicrobial activities of Limonium socotranum and Peperomia blanda extracts. Trop. Biomed. 36(1), 11-21.
  5. Angulo, A.f., R.A. Rosero, and M.S. González. 2012. Estudio etnobotánico de las plantas medicinales utilizadas por los habitantes del corregimiento de Genoy, Municipio de Pasto, Colombia. Univ. Salud 14(2), 168-185.
  6. Brandão, R.M., V.R.F. Ferreira, L.R. Batista, E. Alves, N.A. Lira, B.S. Bellete, J.R.S. Scolforo, and M.D.G. Cardoso. 2020. Antifungal and antimycotoxigenic effect of the essential oil of Eremanthus erythropappus on three different Aspergillus species. Flavour Fragr. J. 35(5), 524-533. Doi: https://doi.org/10.1002/ffj.3588
  7. Cheng, M.-J., S.-J. Lee, Y.-Y. Chang, S.-H. Wu, I.-L. Tsai, B. Jayaprakasam, and I.-S. Chen. 2003. Chemical and cytotoxic constituents from Peperomia sui. Phytochem. 63(5), 603-608. Doi: https://doi.org/10.1016/S0031-9422(03)00183-3
  8. De Díaz, A.M.P., P.P. Díaz, and H. Cardoso. 1988. Volatile constituents of Peperomia subespatulata. Planta Med. 54(1), 92-93. Doi: https://doi.org/10.1055/s-2006-962356
  9. Di Ciaccio, L.S., A.V. Catalano, P.G. López, D. Rojas, D. Cristos, R.H. Fortunato, and A.E. Salvat. 2020. In vitro antifungal activity of Peltophorum dubium (Spreng.) Taub. extracts against Aspergillus flavus. Plants 9(4), 438. Doi: https://doi.org/10.3390/plants9040438
  10. Elyemni, M., B. Louaste, I. Nechad, T. Elkamli, A. Bouia, M. Taleb, M. Chaouch, and N. Eloutassi. 2019. Extraction of essential oils of Rosmarinus officinalis L. by two different methods: hydrodistillation and microwave assisted hydrodistillation. Sci. World J. 3659432. Doi: https://doi.org/10.1155/2019/3659432
  11. Ferreira, E.A., J.B. Reigada, M.V. Correia, M.C.M. Young, E.F. Guimaraes, G.C. Franchi Jr., A.E. Nowill, J.H.G. Lago, L.F. Yamaguvhi, and M.J. Kato. 2014. Antifungal and cytotoxic 2-Acylcyclohexane-1, 3-diones from Peperomia alata and P. trineura. J. Nat. Prod. 77(6), 1377-1382. Doi: https://doi.org/10.1021/np500130x
  12. García-Barriga, H. 1974. Flora medicinal de Colombia: botánica médica. Editorial Tercer Mundo. Bogota.
  13. ISO. 1998a. ISO 279 Essential oils — Determination of relative density at 20 degrees C — Reference method. Geneve, Switzerland.
  14. ISO. 1998b. ISO 280 essential oils — Determination of refractive index. Geneve, Switzerland.
  15. Jardín Botánico de Bogotá José Celestino Mutis. n.d. Plataforma Nombres Comunes: plantas Bogotá. Peperomia subspathulata. In: http://nombrescomunes.jbb.gov.co/; consulted: March, 2022.
  16. Jin, Y.S. 2019. Recent advances in natural antifungal flavonoids and their derivatives. Bioorg. Med. Chem. Lett. 29(19), 126589. Doi: https://doi.org/10.1016/j.bmcl.2019.07.048
  17. Kamatou, G.P.P. and A.M. Viljoen. 2010. A review of the application and pharmacological properties of α-bisabolol and α-bisabolol-rich oils. J. Am. Oil Chem. Soc. 87(1), 1-7. Doi: https://doi.org/10.1007/s11746-009-1483-3
  18. Kfoury, M., A.L.H. Sahraoui, N. Bourdon, F. Laruelle, J. Fontaine, L. Auezova, H. Geige-Gerges, and S. Fourmentin. 2016. Solubility, photostability and antifungal activity of phenylpropanoids encapsulated in cyclodextrins. Food Chem. 196, 518-525. Doi: https://doi.org/10.1016/j.foodchem.2015.09.078
  19. Khan, M.R. and A.D. Omoloso. 2002. Antibacterial activity of Hygrophila stricta and Peperomia pellucida. Fitoterapia 73(3), 251-254. Doi: https://doi.org/10.1016/S0367-326X(02)00066-7
  20. Khan, A., M. Rahman, and S. Islam. 2010. Isolation and bioactivity of a xanthone glycoside from Peperomia pellucida. Life Sci. Med. Res. 2010, LSMR-1.
  21. Langfield, R., F.J. Scarano, M.E. Heitzman, M. Kondo, G.B. Hammond, and C.C. Neto. 2004. Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides. J. Ethnopharmacol. 94(2-3), 279-281. Doi: https://doi.org/10.1016/j.jep.2004.06.013
  22. Marei, G.I.K. and S.A.M. Abdelgaleil. 2018. Antifungal potential and biochemical effects of monoterpenes and phenylpropenes on plant pathogenic fungi. Plant Protect. Sci. 54(1), 9-16. Doi: https://doi.org/10.17221/9/2017-PPS
  23. Ogunmoye, A.O., I.A. Oladosu, O.C. Atewolara-Odule, O.O. Olubomehin, I.B. Onajobi, and S. Tijani. 2018. Antimicrobial activities of essential oils from Peperomia pellucida (Linn.) leaf obtained in Nigeria. J. Chem. Soc. Nigeria 43(4), 872-878.
  24. Okoh, S.O., B.C. Iweriebor, O.O. Okoh, and A.I. Okoh. 2017. Bioactive constituents, radical scavenging, and antibacterial properties of the leaves and stem essential oils from Peperomia pellucida (L.) Kunth. Pharmacogn. Mag. 13(Suppl. 3), 392-400. Doi: https://doi.org/10.4103/pm.pm_106_17
  25. Oloyede, G.K., P.A. Onocha, and B.B. Olaniran. 2011. Phytochemical, toxicity, antimicrobial and antioxidant screening of leaf extracts of Peperomia pellucida from Nigeria. Adv. Environ. Biol. 5(12), 3700-3709.
  26. Peng, Y., S.J. Li, J. Yan, Y. Tang, J.P. Cheng, A.J. Gao, X. Yao, J.J. Ruan, and B.L. Xu. 2021. Research progress on phytopathogenic fungi and their role as biocontrol agents. Front. Microbiol. 12, 670135. Doi: https://doi.org/10.3389/fmicb.2021.670135
  27. Phongtongpasuk, S. and S. Poadang. 2014. Extraction of antioxidants from Peperomia pellucida L. Kunth. Thammasat Int. J. Sci. Tech. 19(3), 38-43.
  28. Poudel, D.K., A. Rokaya, P.K. Ojha, S. Timsina, R. Satyal, N.S. Dosoky, P. Satyal, and W.N. Setzer. 2021. The chemical profiling of essential oils from different tissues of Cinnamomum camphora L. and their antimicrobial activities. Molecule 26(17), 5132. Doi: https://doi.org/10.3390/molecules26175132
  29. Sanabria-Galindo, A., S.I. López, and R. Gualdrón. 1997. Estudio fitoquímico preliminar y letalidad sobre Artemia salina de plantas colombianas. Rev. Colomb. Cienc. Quim.-Farm. 26(1), 15-19.
  30. Sánchez-León, G., A. Vargas-Rincón, and P. Jiménez. 2015. Evaluación de la actividad antifúngica de extractos etanólicos de dos morfotipos de Raphanus raphanistrum L. sobre tres hongos fitopatógenos. Bioagro 27(1), 2-10.
  31. Sil, A., K. Pramanik, P. Samantaray, M.F. Mondal, and V. Yadav. 2020. Essential oils: A boon towards eco-friendly management of phytopathogenic fungi. J. Entomol. Zool. Stud. 8(4), 1884-1891.
  32. Singleton, V.L., R. Orthofer, and R.M. Lamuela-Raventós. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299, 152-178. Doi: https://doi.org/10.1016/S0076-6879(99)99017-1
  33. Soni, A. and S. Sosa. 2013. Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts. J. Pharmacogn. Phytochem. 2(4), 22-29.
  34. Stanojević, L., M. Stanković, V. Nikolić, L. Nikolić, D. Ristić, J. Čanadanovic-Brunet, and V. Tumbas. 2009. Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella L. extracts. Sensors 9(7), 5702-5714. Doi: https://doi.org/10.3390/s90705702
  35. Stashenko, E., C. Ruiz, A. Muñoz, M. Castañeda, and J. Martínez. 2008. Composition and antioxidant activity of essential oils of Lippia origanoides HBK grown in Colombia. Nat. Prod. Commun. 3(4). Doi: https://doi.org/10.1177/1934578X0800300417
  36. Ulloa Ulloa, C., P. Acevedo-Rodríguez, S. Beck, M.J. Belgrano, R. Bernal, P.E. Berry, L. Brako, M. Celis, G. Davidse, R.C. Forzza, S.R. Gradstein, O. Hokche, B. León, S. León-Yánez, R.E. Magill, D.A. Neill, M. Nee, P.H. Raven, H. Stimmel, M.T. Strong, J.L. Villaseñor, J.L. Zarucchi, F.O. Zuluaga, and P.M. Jørgensen. 2017. An integrated assessment of the vascular plant species of the Americas. Science 358(6370), 1614-1617. Doi: https://doi.org/10.1126/science.aao0398
  37. Valente, V.M.M., G.N. Jham, C.M. Jardim, O.D. Dhingra, and I. Ghiviriga. 2015. Major antifungals in nutmeg essential oil against Aspergillus flavus, and A. ochraceus. J. Food Res. 4(1), 51-57. Doi: https://doi.org/10.5539/jfr.v4n1p51
  38. Valero Gutiérrez, Y., L.F. Yamaguchi, M.M. Moraes, C.S. Jeffrey, and M.J. Kato. 2016. Natural products from Peperomia: occurrence, biogenesis and bioactivity. Phytochem. Rev. 15(6), 1009-1033. Doi: https://doi.org/10.1007/s11101-016-9461-5
  39. Villegas, L.F., A. Marçalo, J. Martin, I.D. Fernández, H. Maldonado, A.J. Vaisberg, and G.B. Hammond. 2001. (+)-epi-α-bisabolol is the wound-healing principle of Peperomia galioides: investigation of the in vivo wound-healing activity of related terpenoids. J. Nat. Prod. 64(10), 1357-1359. Doi: https://doi.org/10.1021/np0102859

Downloads

Download data is not yet available.

Similar Articles

<< < 1 2 3 4 

You may also start an advanced similarity search for this article.