Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.)

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Luis Alfonso Causil V.
José Luis Coronado G.
Luis Fernando Verbel M.
María Fernanda Vega J.
Keiner Antonio Donado E.
Carluis Pacheco G.


(NaClO) sodium hypochlorite is a highly toxic chemical compound widely used because of its disinfectant properties. One of the most commonly used plants to detect different types of genetic changes caused by chemical substances is Allium cepa L., in tests for environmental monitoring. The objective of this research was to evaluate the cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium strain L.). Five sodium hypochlorite solutions were prepared at different concentrations, in addition to a control solution, with three replications for each treatment; for 3 days, the growth of the roots was measured; the apices were cut submerging them into hydrochloric acid, subsequently dyed with Aceto-Orcein and were observed under a microscope; the mitotic index (MI) was calculated and the cell anomalies were observed (chromosomal). An analysis of variance (ANOVA) and multiple range tests using the Least Significant Difference method (LSD) were performed. The results showed that there was not a statistically significant difference between the treatments (P


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