Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.)

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Autores

Luis Alfonso Causil V. https://orcid.org/0000-0001-9417-5618
José Luis Coronado G. https://orcid.org/0000-0002-8532-800X
Luis Fernando Verbel M. https://orcid.org/0000-0003-4204-1591
María Fernanda Vega J. https://orcid.org/0000-0002-2986-7527
Keiner Antonio Donado E. https://orcid.org/0000-0003-3383-0564
Carluis Pacheco G. https://orcid.org/0000-0001-9395-4281

Abstract

(NaClO) sodium hypochlorite is a highly toxic chemical compound widely used because of its disinfectant properties. One of the most commonly used plants to detect different types of genetic changes caused by chemical substances is Allium cepa L., in tests for environmental monitoring. The objective of this research was to evaluate the cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium strain L.). Five sodium hypochlorite solutions were prepared at different concentrations, in addition to a control solution, with three replications for each treatment; for 3 days, the growth of the roots was measured; the apices were cut submerging them into hydrochloric acid, subsequently dyed with Aceto-Orcein and were observed under a microscope; the mitotic index (MI) was calculated and the cell anomalies were observed (chromosomal). An analysis of variance (ANOVA) and multiple range tests using the Least Significant Difference method (LSD) were performed. The results showed that there was not a statistically significant difference between the treatments (P

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