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Vol. 12 No. 3 (2018)

Section on aromatic, medicinal and spice plants

Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro

https://doi.org/10.17584/rcch.2018v12i3.7801

Published 2018-09-01

  • David Álvarez-Sánchez
    • ,
  • Andrés Hurtado-Benavides
    • ,
  • Diana Chaves-Morillo
    • ,
  • Danita Andrade-Díaz

David Álvarez-Sánchez
Universidad de Nariño, Facultad de Ciencias Agrícolas, Pasto

Andrés Hurtado-Benavides
Universidad de Nariño, Facultad de Ingeniería Agroindustrial, Pasto

Diana Chaves-Morillo
Universidad de Nariño, Facultad de Ingeniería Agroindustrial, Pasto

Danita Andrade-Díaz
Universidad de Nariño, Facultad de Ciencias Agrícolas, Pasto

Abstract

Rhizoctonia solani is a fungus that affects the production of tubers and vigor of potato plants, Solanum tuberosum L. The environmental consequences generated by its control have driven the search for less contaminating alternatives, and essential oils have great potential. The wild shrub Lippia origanoides H.B.K. (Verbenaceae) has been studied because of its attractive fungicidal properties in its essential extracts; therefore, the objective of this research was to evaluate the biocidal activity of L. origanoides essential oil on R. solani, in vitro. The oil extraction was carried out with steam dragging, the chemical composition was determined with Gas Chromatography-Mass Spectrometry (GC-MS), and, finally, the biocidal activity of the oil was evaluated with the dilution technique in the culture medium, which was complemented with the bioautography technique, which identified thymol as the major compound of the oil (60.7%), responsible for the fungus inhibition. The concentration 110 μL mL-1 limited the growth of R. solani by affecting the structures, thereby demonstrating the biocidal effect of this essential oil and its potential antifungal agent.

Keywords

Oregano, Thymol, Solanum tuberosum, Thin-layer chromatography

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References

  • Abd-El-Khair, H. y El-Gamal, N. 2011. Effects of aqueous extracts of some plant species against Fusarium solani and Rhizoctonia solani in Phaseolus vulgaris plants. Archives of Phytopathol. Plant Prot., 44, 1–16.
  • Álvarez, D., Hurtado, A. y Acosta, J. 2016. Aceite esencial de Lippia origanoides como posible producto biorracional en el cultivo de papa (Solanum tuberosum). Vitae. 23(1), 526–530.
  • Amini, M., Safaie, N., Salmano, M. and Shams, M. 2012. Antifungal activity of three medicinal plant essential oils against some phytopathogenic fungi. Trakia J.l of Sci., 10(1), 1–8.
  • Arango, O., Bolaños, F., Villota, O., Hurtado, A. y Toro, I. 2012. Optimization of yield and thymol content of wild oregano essential oil obtained by steam distillation process. Biotecnol. en el Sect. Agropecu. y agroindustrial. 10(2), 217–226.
  • Arango, O., Hurtado, A., Pantoja, D. y Santacruz, L. 2014. Actividad inhibitoria del aceite esencial de Lippia origanoides H.B.K sobre el crecimiento de Phytophthora infestans. Acta Agron., 64(2), 116–124.
  • Barbosa, F., Barbosa, L., Melo, E., Botelho, F. y Santos, R. 2006. Influência da temperatura do ar de secagem sobre o teor e a composição química do óleo essencial de Lippia alba (Mill) N. E. Brown. Química Nova. 29(6), 1221–1225.
  • Bittara, F., Rodríguez, D., Sanabria, M., Monroy, J. y Rodríguez, J. L. 2009. Evaluación de fungicidas y productos vegetales en el combate de la sarna polvorienta de la papa. Interciencia. 34(4), 265–269.
  • Hoyos, J., Alves, E., Rozwalka, L., Souza, E. y Zeviani, W. 2012. Antifungal activity and ultrastructural alterations in Pseudocercospora griseola treated with essential oils. Ciência e Agrotecnologia. 36(3). 270–284.
  • Kankam, F., Qiu, H., Pu, L., Long, H., Zhang, C., He, J. y Zhang, H. 2016. Isolation, purification and characterization of phytotoxins Produced by Rhizoctonia solani AG-3, the cause agent of potato stem canker. Am. J. Potato Res. 93(4), 321–330.
  • Khaledi, N., Taheri, P. y Tarighi, S. 2015. Antifungal activity of various essential oils against Rhizoctonia solani and Macrophomina phaseolina as major bean pathogens. J. Appl. Microbiol. 118(3), 704–717. doi: 10.1111/jam.12730.
  • Marei, G., Abdel, M. y Abdelgaleil, S. 2012. Comparative antifungal activities and biochemical effects of monoterpenes on plant pathogenic fungi. Pestic. Biochem. Physio. 103(1), 56–61.
  • Medeiros, F, Gouveia, F., Bizzo, H., Vieira, R. y Del Menezzi, C. 2016. Fungicidal activity of essential oils from Brazilian Cerrado species against wood decay fungi. Int. Biodeterior. Biodegradation. 114(2016), 87–93. doi: https://doi.org/10.1016/j.ibiod.2016.06.003.
  • Medeiros, H., de Lima, I., Nunes, K., Osório, L., de Almeida, R., dos Santos, B., Melo, H., Lira, A., Freire, M., Lopes, A. y Dantas, J. 2014. Effect of Lippia origanoides H.B.K. essential oil in the resistance to aminoglycosides in methicillin resistant Staphylococcus aureus. Eur. J. Integr. Med. 6(5), 560–564.
  • Moreira, M., Newandram, G., Dev, O., Manrangon, C., Coura, R. y Moreira, V. 2011. Composition, antifungal activity and main fungitoxic components of the essential oil of Mentha piperita L. J. food Saf. 32(1), 29–36.
  • Nguyen, V., Nguyen, D., Seo, D., Park, R. y Jung, W. 2009. Antimycotic activities of Cinnamon-derived compounds against Rhizoctonia solani in vitro. BioControl. 54(5), 697–707.
  • Ospina, J., Grande, C., Menjivar, J. y Sanchez, M. 2016. Relación entre índice de refracción y la concentración de Timol en aceites esenciales de Lippia origanoides Kunth. Chil. J. Agric. Anim. Sci. ex Agro-Ciencia. 32(2), 127–133.
  • Plodpai, P., Chuenchitt, S., Petcharat, V., Chakthong, S. y Voravuthikunchai, S. 2013. Anti-Rhizoctonia solani activity by Desmos chinensis extracts and its mechanism of action. Crop Prot. 43, 65–71.
  • Ramírez, L., Isaza, J., Veloza, L., Stashenko, E. y Marín, D. 2009. Actividad antibacteriana de aceites esenciales de Lippia origanoides de diferentes orígenes de Colombia. Ciencia. 17(4), 313–321.
  • Rodriguez, D. y Sanabria, M. 2005. Efecto del extracto de tres plantas silvestres sobre la Rizoctoniasis, la mancha sureña del maíz y los patógenos que las causan. Interciencia. 30(12), 739–744.
  • Seema, M. y Devaki, N. 2010. Effect of some essential oils on Rhizoctonia solani Kuhn infecting flue-cured virginia tobacco. J. Biopestic. 3(3), 563–566.
  • Souza, E., Oliveira, C., Stamford, T., Conceição, M. y Gomes, N. 2013. Influence of carvacrol and thymol on the physiological attributes, enterotoxin production and surface characteristics of Staphylococcus aureus strains isolated from foods. Brazilian J. Microbiol. 44(1), 29–35.
  • Stashenko, E., Martínez, J., Ruíz, C., Arias, G., Durán, C., Salgar, W. y Cala, M. 2010. Lippia origanoides chemotype differentiation based on essential oil GC-MS and principal component analysis. J. Sep. Sci. 33(1), 93–103.
  • Taborda, L., Sanchez, M., Bonilla, R. y Huertas, C. 2015. Efecto fungistático de extractos y aceites esenciales de Lippia origanoides HBK y Thymus vulgaris L. como alternativas de manejo de Botrytis cinerea en fresa. Acta Agron. 64(1), 93–99.
  • Tangarife, V., Roa, V., Betancur, L., Durán, D., Stashenko, E. y Mesa, A. 2012. Antifungal activity of Verbenaceae and Labiatae families essential oils. Pharmacologyonline. 1, 43–55.
  • Vicuña, G., Stashenko, E. y Fuentes, J. 2010. Chemical composition of the Lippia origanoides essential oils and their antigenotoxicity against bleomycin-induced DNA damage. Fitoterapia, 81(5), 343–349.
  • Villa, A., Pérez, R., Morales, H., Basurto, M., Soto, J. y Martínez, E. 2015. Situación actual en el control de Fusarium spp. y evaluación de la actividad antifúngica de extractos vegetales. Acta Agron. 64(2), 194–205.

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