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Vol. 19 No. 1 (2025)
In Press / En prensa

Vegetable section

Molecular detection of three RNA viruses in tomato (Solanum lycopersicum L.) leaf tissue and seeds in Antioquia (Colombia)

https://doi.org/10.17584/rcch.2025v19i1.18368

Published 2025-01-01

  • Juan Pablo Bados
    • ,
  • Jazmín Gómez
    • ,
  • Alejandra Pérez
    • ,
  • Jhoan Salazar
    • ,
  • Mauricio Marín

Juan Pablo Bados
Universidad Nacional de Colombia, Sede Medellín, Facultad de Ciencias, Laboratorio de Microbiología Industrial, Medellin (Colombia)

Jazmín Gómez
Universidad Nacional de Colombia, Sede Medellín, Facultad de Ciencias, Laboratorio de Microbiología Industrial, Medellin (Colombia)

Alejandra Pérez
Universidad Nacional de Colombia, Sede Medellín, Facultad de Ciencias, Laboratorio de Microbiología Industrial, Medellin (Colombia)

Jhoan Salazar
Universidad Nacional de Colombia, Sede Medellín, Facultad de Ciencias, Laboratorio de Microbiología Industrial, Medellin (Colombia)

Mauricio Marín
Universidad Nacional de Colombia, Sede Medellín, Facultad de Ciencias, Laboratorio de Microbiología Industrial, Medellin (Colombia)

RT-PCR detection of PYVV using primers PYVV_F_CP/ PYVV_R_CP. Photo: J.P. Bados

Abstract

Viruses are a significant threat to tomato crops (Solanum lycopersicum) worldwide. In Colombia, 20 species of viruses from 10 genera have been reported in tomato, with 9 of them being RNA viruses that cause varying levels of damage. This study in Antioquia evaluated the presence of three RNA viruses: potato virus Y (PVY), potato yellow vein virus (PYVV) and Southern tomato virus (STV) in different tomato seeds and plants using RT-qPCR, conventional RT-PCR and Sanger sequencing. The study included 15 leaf tissue samples of the Chonto variety and 15 seed samples of various tomato varieties (Cherry, Chonto, Yellow, Kidney, San Marzano, and Mexican husk tomato). The results showed high levels of PVY in leaf tissue (93.3%) and seeds (46.6%), while PYVV was detected in 60% of leaf samples and 26.6% of seed samples. STV was found in 93.3% of seeds but was not detected in any leaf samples. Sanger sequencing confirmed the presence of these viruses, and phylogenetic analyses placed the isolates in clades previously reported for these viruses in Colombia. These findings underscore the need for a tomato seed certification program in Colombia to ensure viral-free seeds.

Keywords

Diagnosis, Plant virology, RT-qPCR, Sequencing, Solanaceae

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