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Vol. 17 Núm. 3 (2023)

Sección de otras especies

Selección y evaluación de mutantes knockout genéticamente editados de homólogos de AtAAP2 y AtCRF4 de arroz para la eficiencia agronómica del uso del nitrógeno (ANUE)

https://doi.org/10.17584/rcch.2023v17i3.16120

Publicado 2023-09-01

  • Kentaro Wakatabi
    • ,
  • Michael Gomez Selvaraj
    • ,
  • Diego Alexander Guzmán-Prada
    • ,
  • Juan Bosco Cuásquer
    • ,
  • Karina López-López
    • ,
  • Masaki Endo
    • ,
  • Manabu Ishitani

Kentaro Wakatabi
Universidad Nacional de Colombia, Facultad de Ciencias Agropecuarias, Palmira

Michael Gomez Selvaraj
Centro Internacional de Agricultura Tropical, Cali

Diego Alexander Guzmán-Prada
Centro Internacional de Agricultura Tropical, Cali

Juan Bosco Cuásquer
Centro Internacional de Agricultura Tropical, Cali

Karina López-López
Universidad Nacional de Colombia, Facultad de Ciencias Agropecuarias, Palmira

Masaki Endo
National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, Tsukuba

Manabu Ishitani
Centro Internacional de Agricultura Tropical, Cali

Gene-edited rice lines with different phenotypic features. Photo: K. Wakatabi

Resumen

El nitrógeno (N) es esencial para la síntesis de aminoácidos en la producción de arroz, pero su uso excesivo plantea una preocupación ambiental. Esta investigación tuvo como objetivo mejorar la eficiencia agronómica del uso del nitrógeno (ANUE) en el arroz mediante “knockout (KO)” de homólogos en arroz de los dos genes seleccionados de Arabidopsis thaliana: AtAAP2, una permeasa de aminoácidos involucrada en el transporte de N en los brotes, y AtCRF4, un factor de transcripción que participa en la absorción de N en las raíces. Los homólogos de estos genes en el arroz se identificaron por la similitud de la secuencia de aminoácidos y se desactivaron mediante la edición genética (GE) mediada por CRISPR/Cas9. Las líneas AAP2-KO y CRF4-KO fueron sometidas a evaluaciones agronómicas con tres dosis de N: 100% (180 kg ha-1), 50% (90 kg ha-1) y 0% (0 kg ha-1) y mostraron un aumento de 130-175% en peso de biomasa seca y un aumento de 183-313% en número de panículas en comparación con el control (WT) en el primer experimento. Estas líneas también tenían una senescencia foliar más lenta, el denominado rasgo de "permanecer verde", lo que indica el efecto de desactivación de genes del objetivo en el metabolismo del N. Sin embargo, ni AAP2-KO ni CRF4-KO mostraron mejor rendimiento o ANUE que WT. Este estudio demostró la utilidad de la tecnología de edición genética en la evaluación de genes y destacó los efectos de los genes AtAAP2 y AtCRF4 en el ciclo del N de la planta.

Palabras clave

Aminoácido permeasa 2 (AAP2), Factor de respuesta de citoquinina 4 (CRF4), Oryza sativa L., Sensores remotos

PDF (English)

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