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Vol. 16 Núm. 1 (2025)
Vol. 16 Núm. 1 (2025): Vol. 16 Núm. 1 (2025): Vol 16, Núm.1 (2025): Enero - Junio

Artículos de investigación / Research papers

IDENTIFICACIÓN MOLECULAR DE GRUPOS DE ANASTOMOSIS DE Rhizoctonia solani Kühn CAUSANTE DE COSTRA NEGRA DE LA PAPA EN NARIÑO (COLOMBIA)

https://doi.org/10.19053/uptc.01217488.v16.n1.2025.17646

Publicado 2025-04-20

  • Claudia Quiroz

Claudia Quiroz
Universidad de nariño

Resumen

Rhizoctonia solani Kühn causante de costra negra en papa, ha aumentado su incidencia en Nariño, dificultando su manejo. El desconocimiento de los grupos de anastomosis (AG) presentes en sus poblaciones construyen a esta situación, pues influyen en el desempeño de las estrategias de control. Por lo tanto, se planteó evaluar la variabilidad genética de R. solani en Nariño, a partir de la identificación molecular de sus AG. Se analizaron 287 aislamientos procedentes de papa, de estos, 116 con PCR y cebadores ITS1-ITS4 y toda la colección, con PCR y cebadores específicos para AG-2-1, AG-3 PT y AG-4. El análisis filogenético se hizo con Máxima Verosimilitud, bootstrap de 1000 réplicas y modelo Kimura 2-parámetros. En la caracterización molecular con ITS, el 100 % de las secuencias coincidieron con R. solani y se identificó a AG-3 (46), AG-3 PT (69) y AG-2-1 (1). Los cebadores específicos determinaron la presencia de AG-3 PT en 281 y AG-2-1 en seis aislamientos del patógeno. R. solani AG-2-1 se reporta por primera vez en Nariño. El análisis filogenético agrupó los aislamientos en siete clados, independientemente de la procedencia, lo que comprueba la existencia de variabilidad genética intraespecífica en las poblaciones de R. solani presentes en Nariño.

Palabras clave

Solanum tuberosum, Cancro del tallo, Variabilidad genética, PCR, cebadores

Citas

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