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Vol. 1 No. E (2024)
Segunda Jornada de Catálisis

Artículos de investigación / Research papers

Metabolic Dynamics Of Bacterial Strains In Eichhornia crassipes Hydrolysate: A Comparative Study Of Growth And Lactic Acid Production

https://doi.org/10.19053/uptc.01217488.v1.nE.2024.18273

Published 2024-11-15

  • Andres Felipe Monroy Ramirez
    • ,
  • Aida J. Fonseca
    • ,
  • Angie P. Sotelo
    • ,
  • Gerardo A. Caicedo

Andres Felipe Monroy Ramirez
Universidad Pedagógica y Tecnológica de Colombia

Aida J. Fonseca
Universidad Santo Tomas

Angie P. Sotelo
Universidad Pedagógica y Tecnologica de Colombia

Gerardo A. Caicedo
Universidad Pedagógica y Tecnológica de Colombia

Abstract

In the present investigation, the growth conditions in a fermentation process at 35 °C, 180 rpm for 60 h, of four strains of commercial bacteria DRI SET 432, DRI FAS 992, Bacillus subtilis, and water kefir were compared in two growth media (M1 base growth medium and M2 medium supplemented with E. crassipes hydrolysate). The metabolic response of the strains in the media was monitored and evaluated by means of biomass production by the Mc Farland turbidimetric method (cells.mL-1), sugar consumption (g.L-1) DNS method and lactic acid production (%) NTC 4978, these controls were performed every 12 h. The strains evaluated presented their growth phase in the growth media. The strains evaluated presented their exponential phase at 12 h in M1 and M2. We found a decrease in biomass and lactic acid production in the fermentation processes with M2 for DRI SET 432, DRI FAS 992 and water kefir, and a greater growth with Bacillus subtilis (32 x 108 cells/mL) at 60 h. On the other hand, the highest lactic acid yield was presented with strain FAS 992 (streptococcus salivarius sub. thermophilus) with 1.390 g lactic acid/ g substrate consumed in M1 and in Water kefir with 0.753 g lactic acid/ g substrate consumed for M2.

Keywords

acid hydrolysis, alkaline pretreatment, Bacillus sp., Eichhornia crassipes, lactic acid bacteria

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