Skip to main navigation menu Skip to main content Skip to site footer

Physiological and morphological features denote that salt stress in Stevia rebaudiana is based on nonstomatic instead of stomatic limitation

Influence of NaCl salinity on plant dry weight of different organs G04 (A) and G19 (B) genotypes of S. rebaudiana plants. Arrow indicates the salinity resistance threshold (i.e. inflection curve point), while the dotted line marks the C50 values. LDW: Leaf dry weight. SDW: shoot dry weight. RDW: root dry weight.

Abstract

Stevia rebaudiana is a very widespread plant throughout the world, due to the production of diterpenic steviol glycosides, with high sweetening power, being up to 300 times sweeter than sugar at a concentration of 0.4% (w/v). In the Colombian Caribbean, research has focused on evaluating the adaptation and management of commercial genotypes and experimental lines, however, their behavior against soil salinization is unknown. The objective of this research was to evaluate the response of some physiological and morphological characteristics of two promising genotypes against salt stress. The relationship between gas exchange, chlorophyll a fluorescence, and growth parameters was modulated as a function of the NaCl concentration. Although stomatal conductance and perspiration did not show a response to salt levels, the reduction in net photosynthesis as salinity increased was not associated with stomatal effects, but rather with a drop in the rate of electron transport, which articulates the absorption of light energy and its conversion into photoassimilates. Therefore, it could be inferred that salinity-stressed S. rebaudiana plants are more sensitive to non-stomatal limitation than to stomatal limitation.

Keywords

Biomass distribution, Disruption between energy input and photosynthesis, Gas exchange, Salinity, Stomatal conductance

PDF

References

  • Acosta-Motos, J., L. Noguera-Vera, G. Barba-Espín, A. Piqueras, and J. Hernández. 2019. Antioxidant metabolism and chlorophyll fluorescence during the acclimatisation to ex vitro conditions of micropropagated Stevia rebaudiana Bertoni plants. Antioxidants 8(12), 615. Doi: 10.3390/antiox8120615
  • Basharat, S., Z. Huang, M. Gong, X. Lv, A. Ahmed, I. Hussain, J. Li, G. Du, and L. Liu. 2021. A review on current conventional and biotechnical approaches to enhance biosynthesis of steviol glycosides in Stevia rebaudiana. Chin. J. Chem. Eng. 30, 92-104. Doi: 10.1016/j.cjche.2020.10.018
  • Batista, K., W. Araújo, W. Antunes, P. Cavatte, G. Moraes, S. Martins, and F. DaMatta. 2012. Photosynthetic limitations in coffee plants are chiefly governed by diffusive factors. Trees 26, 459-468. Doi: 10.1007/s00468-011-0606-2
  • Brown, M. and A. Forsythe. 1974. Robust tests for equality of variances. J. Am. Stat. Assoc. 69(346), 364-367. Doi: 10.2307/2285659
  • Cantabella, D., A. Piqueras, J. Acosta-Motos, A. Bernal-Vicente, J. Hernández, and P. Díaz-Vivancos. 2017. Salt-tolerance mechanisms induced in Stevia rebaudiana Bertoni: Effects on mineral nutrition, antioxidative metabolism and steviol glycoside content. Plant Physiol Biochem. 115, 484-496. Doi: 10.1016/j.plaphy.2017.04.023
  • Centritto, M., F. Loreto, and K. Chartzoulakis. 2003. The use of low [CO2] to estimate diffusional and non-diffusional limitations of photosynthetic capacity of salt-stressed olive saplings. Plant Cell Environ. 26(4), 585-594. Doi: 10.1046/j.1365-3040.2003.00993.x
  • Cerqueira, J., J. Silveira, F. Carvalho, J. Cunha, and M. Neto. 2019. The regulation of P700 is an important photoprotective mechanism to NaCl‐salinity in Jatropha curcas. Physiol. Plant. 167(3), 404-417. Doi: 10.1111/ppl.12908
  • Chaves, M.M., J. Flexas, and C. Pinheiro. 2008. Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann. Bot. 103(4), 551-560. Doi: 10.1093/aob/mcn125
  • Debnath, M., N. Ashwath, and D. Midmore. 2019. Physiological and morphological responses to abiotic stresses in two cultivars of Stevia rebaudiana (Bert.) Bertoni. South Afr. J. Bot. 123, 124-132. Doi: 10.1016/j.sajb.2019.01.025
  • Dos Santos, O., K. Mendes, S. Martins, W. Batista-Silva, M. dos Santos, J. Figueiroa, E. Souza, D. Fernandes, W. Araujo, and M. Pompelli. 2019. Physiological parameters and plasticity as key factors to understand pioneer and late successional species in the Atlantic Rainforest. Acta Physiol. Plant. 41, 145. Doi: 10.1007/s11738-019-2931-9
  • Flexas, J. and H. Medrano. 2002. Drought‐inhibition of photosynthesis in C3 plants: Stomatal and non‐stomatal limitations revisited. Ann. Bot. 89(2), 183-189. Doi: 10.1093/aob/mcf027
  • Fonseca-Pereira, P., D. Daloso, J. Gago, A. Nunes-Nesi, and W. Araújo. 2019. On the role of the plant mitochondrial thioredoxin system during abiotic stress. Plant Signal Behav. 14(6), 1592536. Doi: 10.1080/15592324.2019.1592536
  • González, J., S. Eisa, S. Hussin, and F. Prado. 2015. Quinoa: An incan crop to face Global changes in agriculture. pp. 1-18. In: Murphy, K. and J. Matanguiban (eds.). Quinoa: Improvement and sustainable production. Wiley Blackwell, Hoboken, NJ. Doi: 10.1002/9781118628041.ch1
  • Hernández-Fernandéz, I., A. Jarma-Orozco, and M. Pompelli. 2021. Allometric models for non-destructive leaf area measurement of stevia: an in depth and complete analysis. Hortic. Bras. 39(2), 205-215. Doi: 10.1590/s0102-0536-20210212
  • Holbrook, N. 2015. Water balance of plants. pp. 99-118. In: Taiz, L., E. Zeiger, I. Moller, and A. Murphy (eds). Plant physiology and development. 6th ed. Sinauer Associates, Oxford.
  • Hsie, B., K. Mendes, W. Antunes, L. Endres, M. Campos, F. Souza, N. Santos, B. Singh, E. Arruda, and M. Pompelli. 2015. Jatropha curcas L. (Euphorbiaceae) modulates stomatal traits in response to leaf-to-air vapor pressure deficit. Biomass Bioenerg. 81, 273-281. Doi: 10.1016/j.biombioe.2015.07.014
  • Huang, L., Z. Li, Q. Liu, G. Pu, Y. Zhang, and J. Li. 2019. Research on the adaptive mechanism of photosynthetic apparatus under salt stress: New directions to increase crop yield in saline soils. Ann. Appl. Biol. 175, 1-17. Doi: 10.1111/aab.12510
  • Hussin, S., N. Geissler, M. El-Far, and H.-W. Koyro. 2017. Effects of salinity and short-term elevated atmospheric CO2 on the chemical equilibrium between CO2 fixation and photosynthetic electron transport of Stevia rebaudiana Bertoni. Plant Physiol. Biochem. 118, 178-186. Doi: 10.1016/j.plaphy.2017.06.017
  • Jarma-Orozco, A., E. Combatt-Caballero, and J. Jaraba.-Navas. 2020. Growth and development of Stevia rebaudiana Bert., in high and low levels of radiation. Curr. Plant Biol. 22, 100144. Doi: 10.1016/j.cpb.2020.100144
  • Mendes, K.R., J.A.A. Granja, J.P. Ometto, A.C.D. Antonio, R.S.C. Menezes, E.C. Pereira, and M.F. Pompelli. 2017. Croton blanchetianus modulates its morphophysiological responses to tolerate drought in a tropical dry forest. Func. Plant Biol. 44(10), 1039-1051. Doi: 10.1071/FP17098
  • Osmond, B., M. Badger, K. Maxwell, O. Björkman, and R. Leegood. 1997. Too many photons: photorespiration, photoinhibition and photooxidation. Trends Plant Sci. 2(4), 119-121. Doi: 10.1016/S1360-1385(97)80981-8
  • Pan, T., M. Liu, V. Kreslavski, S. Zharmukhamedov, C. Nie, M. Yu, V. Kuznetsov, S. Allakhverdiev, and S. Shabala. 2021. Non-stomatal limitation of photosynthesis by soil salinity. Crit Rev Environ. Sci. Technol. 51(8), 791-825. Doi: 10.1080/10643389.2020.1735231
  • Pinho-Pessoa, A.C.B., K.R. Mendes, A. Jarma-Orozco, M.P.S. Pereira, M.A. Santos, R.S.C. Menezes, J.P. Ometto, E.C. Pereira, and M.F. Pompelli. 2018. Interannual variation in temperature and rainfall can modulate the physiological and photoprotective mechanisms of a native semiarid plant species. Indian J. Sci. Technol. 11(41), 1-17. Doi: 10.17485/ijst/2018/v11i42/130972
  • Pompelli, M., R. Barata-Luís, H. Vitorino, E. Gonçalves, E. Rolim, M. Santos, J. Almeida-Cortez, V. Ferreira, E. Lemos, and L. Endres. 2010. Photosynthesis, photoprotection and antioxidant activity of purging nut under drought deficit and recovery. Biomass Bioenerg. 34(8), 1207-1215. Doi: 10.1016/j.biombioe.2010.03.011
  • Putnik, P., I. Bezuk, F. Barba, J. Lorenzo, I. Polunić and D. Bursać. 2020. Sugar reduction: Stevia rebaudiana Bertoni as a natural sweetener. pp. 123-152. In: Barba, F., P. Putnik, and D. Kovačević (eds.). Agri-food industry strategies for healthy diets and sustainability. Academic Press, San Diego, CA. Doi: 10.1016/B978-0-12-817226-1.00005-9
  • Ramesh, K.,V. Singh, and N. Megeji. 2006. Cultivation of stevia [Stevia rebaudiana (Bert.) Bertoni]: a comprehensive review. Adv. Agron. 89, 137-177. Doi: 10.1016/S0065-2113(05)89003-0
  • Reis, M., L. Coelho, G. Santos, U. Kienle, and J. Beltrão. 2015. Yield response of stevia (Stevia rebaudiana Bertoni) to the salinity of irrigation water. Agr. Water Manage. 152, 217-221. Doi: 10.1016/j.agwat.2015.01.017
  • Shahverdi, M., H. Omidi, and S. Tabatabaei. 2019. Stevia (Stevia rebaudiana Bertoni) responses to NaCl stress: Growth, photosynthetic pigments, diterpene glycosides and ion content in root and shoot. J. Saudi Soc. Agric. Sci. 18(4), 355-360. Doi: 10.1016/j.jssas.2017.12.001
  • Shapiro, S. and M. Wilk. 1965. An analysis of variance test for normality (complete samples). Biometrika. 52(3/4), 591-611. Doi: 10.2307/2333709
  • Silva-Santos, L., N. Corte-Real, J. Dias-Pereira, R. Figueiredo, L. Endres, and M. Pompelli. 2019. Salinity shock in Jatropha curcas leaves is more pronounced during recovery than during stress time. Braz. J. Dev. 5, 11359-11369. Doi: 10.1101/378208
  • Yu, B., J. Niu, J. Feng, M. Xu, X. Xie, W. Gu, S. Gao, and G. Wang. 2018. Regulation of ferredoxin-NADP+ oxidoreductase to cyclic electron transport in high salinity stressed Pyropia yezoensis. Front. Plant Sci. 9, 1092. Doi: 10.3389/fpls.2018.01092
  • Zeng, J., A. Chen, D. Li, B. Yi, and W. Wu. 2013. Effects of salt stress on the growth, physiological responses, and glycoside contents of Stevia rebaudiana Bertoni. J. Agric. Food Chem. 61(24), 5720-5726. Doi: 10.1021/jf401237x

Downloads

Download data is not yet available.

Similar Articles

1 2 3 > >> 

You may also start an advanced similarity search for this article.