Effect of biostimulants on dry matter accumulation and gas exchange in plantain plants (Musa AAB)
- Diana Mateus-Cagua
, - Gustavo Rodríguez-Yzquierdo
Abstract
Biostimulants can potentially improve plant growth and development, modifying physiological processes. This study evaluated the effect of four biostimulants on the growth of ‘Hartón’ plantain plants and the leaf gas exchange during the vegetative phase. This experiment was developed on a plantain farm’s nursery in Fuente de Oro (Colombia) with a randomized complete block design with four replicates. The treatments were the biostimulants: Bactox WP®: Bacillus subtilis (Bs); Baliente®: Bacillus amyloliquefaciens (Ba); Tierra Diatomeas®: silicon dioxide (Si); Re-Leaf®: salicylic acid (SA) and the control (water). All products had a positive effect on the accumulation of total dry matter (DM) (between 58.4 and 21.9%) and on the photosynthetic activity (a maximum of 110 and 24.3% in first and second evaluation), as compared to the control, while no differences were found (P>0.05) for the foliar emission rate and chlorophyll content between the treatments. The plants treated with Bs had the greatest DM accumulation at the end of the study and a constant, high photosynthetic activity. All the while Bs, Ba and Si managed to stimulate greater early photosynthetic activity. According to the results, the use of these biostimulants during the vegetative phase had an effect on the physiological processes that enhance DM accumulation in plantain plants, which could be potentially useful for the transplanting stage and increase the reserves used during their establishment and development in the field.
Keywords
Photoassimilates distribution, ‘Hartón’ plantain, Plant growth promoting rhizobacteria (PGPR), Bacillus, Silicon, Salicylic acid
References
Agarie, S., H. Uchida, W. Agata, F. Kubota, and P.B. Kaufman. 1998. Effects of silicon on transpiration and leaf conductance in rice plants (Oryza sativa L). Plant Prod. Sci. 1(2), 89-95. Doi: 10.1626/pps.1.89
Agarwal, P., P.C. Singh, V. Chaudhry, P.A. Shirke, D. Chakrabarty, A. Farooqui, C.S. Nautiyal, A.P. Sane, and V.A. Sane. 2019. PGPR-induced OsASR6 improves plant growth and yield by altering root auxin sensitivity and the xylem structure in transgenic Arabidopsis thaliana. J. Plant Physiol. 240, 153010. Doi: 10.1016/j.jplph.2019.153010
Ahmad, Z., J. Wu, L. Chen, and W. Dong. 2017. Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR. Sci. Rep. 7, 1777. Doi: 10.1038/s41598-017-01940-9
Anusuya, P. 2014. Studies on screening of banana genotypes against salt and water deficit stresses. PhD thesis. Tamil Nadu Agricultural University, Coimbatore, India.
Asari, S., D. Tarkowská, J. Rolčík, O. Novák, D.V. Palmero, S. Bejai, and J. Meijer. 2017. Analysis of plant growth-promoting properties of Bacillus amyloliquefaciens UCMB5113 using Arabidopsis thaliana as host plant. Planta 245(1), 15-30. Doi: 10.1007/s00425-016-2580-9
Aucique-Pérez, C.E., P.E. Menezes Silva, W.R. Moreira, F.M. DaMatta, and F.Á. Rodrigues. 2017. Photosynthesis impairments and excitation energy dissipation on wheat plants supplied with silicon and infected with Pyricularia oryzae. Plant Physiol. Biochem. 121, 196-205. Doi: 10.1016/j.plaphy.2017.10.023
Buah, J.N. and J.W. Tachie-Menson. 2015. Suitability of bud manipulation technique as an alternative to tissue culture in the production of suckers for plantains and bananas. Biotechnol. 14(1), 41-46. Doi: 10.3923/biotech.2015.41.46
Bulgari, R., G. Cocetta, A. Trivellini, P. Vernieri, and A. Ferrante. 2015. Biostimulants and crop responses: a review. Biol. Agric. Hort. 31(1), 1-17. Doi: 10.1080/01448765.2014.964649
Calvo, P., L. Nelson, and J.W. Kloepper. 2014. Agricultural uses of plant biostimulants. Plant Soil 383(1-2), 3-41. Doi: 10.1007/s11104-014-2131-8
Calvo, P., D.B. Watts, J.W. Kloepper, and H.A. Torbert. 2017. Effect of microbial-based inoculants on nutrient concentrations and early root morphology of corn (Zea mays). J. Plant Nutr. Soil Sci. 180(1), 56-70. Doi: 10.1002/jpln.201500616
Cao, W.L., X.C. Meng, and W. Ma. 2015. Effect of salicylic acid on photosynthesis, physio-biochemistry and quality of Panax ginseng under full sun shine in spring. China J. Chin. Materia Med. 40(18), 3553-3559. Doi: 10.4268/cjcmm20151808
Chaves, B., G. Cayón, and J.W. Jones. 2009. Modeling plantain (Musa AAB Simmonds) potential yield. Agron. Colomb. 27(3), 359-366.
Detmann, K.C., W.L. Araújo, S.C.V. Martins, L.M.V.P. Sanglard, J.V. Reis, E. Detmann, F.Á. Rodrigues, A. Nunes-Nesi, A.R. Fernie, and F.M. DaMatta. 2012. Silicon nutrition increases grain yield, which, in turn, exerts a feed-forward stimulation of photosynthetic rates via enhanced mesophyll conductance and alters primary metabolism in rice. New Phytologist 196(3), 752-762. Doi: 10.1111/j.1469-8137.2012.04299.x
Du Jardin, P. 2015. Plant biostimulants: definition, concept, main categories and regulation. Sci. Hortic. 196, 3-14. Doi: 10.1016/j.scienta.2015.09.021
Elhakem, A.H. 2019. Impact of salicylic acid application on growth, photosynthetic pigments and organic osmolytes response in Mentha arvensis under drought stress. J. Biol. Sci. 19(6), 372-380. Doi: 10.3923/jbs.2019.372.380
FAOSTAT. 2016. Agriculture data. In: http://www.fao.org/faostat/en/#data/QC; consulted: Mach, 2018.
Galán-Saúco, V. and J. C. Robinson. 2013. Fisiología, clima y producción de banano. pp. 44-56. In: XX Reunião Internacional da Associação para a Cooperação em Pesquisa e Desenvolvimento Integral das Musáceas (Bananas e Plátanos). Fortaleza, Brazil.
Gao, X., C. Zou, L. Wang, and F. Zhang. 2006. Silicon decreases transpiration rate and conductance from stomata of maize plants. J. Plant Nutr. 29(9), 1637-1647. Doi: 10.1080/01904160600851494
Gemin, L.G., Á.F. Mógor, J.D.O. Amatussi, and G. Mógor. 2019. Microalgae associated to humic acid as a novel biostimulant improving onion growth and yield. Sci. Hortic. 256, 108560. Doi: 10.1016/j.scienta.2019.108560
Halpern, M., A. Bar-Tal, M. Ofek, D. Minz, T. Müller, and U. Yermiyahu. 2015. The use of biostimulants for enhancing nutrient uptake. Adv. Agron. 130, 141-174. Doi: 10.1016/bs.agron.2014.10.001
Helaly, M.N., H. El-Hoseiny, N.I. El-Sheery, A. Rastogi, and H.M. Kalaji. 2017. Regulation and physiological role of silicon in alleviating drought stress of mango. Plant Physiol. Biochem. 118, 31-44. Doi: 10.1016/j.plaphy.2017.05.021
Hooks, C.R.R., M.G. Wright, D.S. Kabasawa, R. Manandhar, and R.P.P. Almeida. 2008. Effect of banana bunchy top virus infection on morphology and growth characteristics of banana. Ann. Appl. Biol. 153(1), 1-9. Doi: 10.1111/j.1744-7348.2008.00233.x
Jalal, R.S., S.O. Bafeel, and A.E. Moftah. 2012. Effect of salicylic acid on growth, photosynthetic pigments and essential oil components of Shara (Plectranthus tenuiflorus) plants grown under drought stress conditions. Int. Res. J. Agric. Sci. Soil Sci. 2(6), 252-260.
Janda, T., O.K. Gondor, R. Yordanova, G. Szalai, and M. Pál. 2014. Salicylic acid and photosynthesis: signalling and effects. Acta Physiol. Plant. 36(10), 2537-2546. Doi: 10.1007/s11738-014-1620-y
Jang, J.H., S.-H. Kim, I. Khaine, M.J. Kwak, H.K. Lee, T.Y. Lee, W.Y. Lee, and S.Y. Woo. 2018. Physiological changes and growth promotion induced in poplar seedlings by the plant growth-promoting rhizobacteria Bacillus subtilis JS. Photosynthetica 56(4), 1188-1203. Doi: 10.1007/s11099-018-0801-0
Kauffman, G.L., D.P. Kneivel, and T.L. Watschke. 2007. Effects of a biostimulant on the heat tolerance associated with photosynthetic capacity, membrane thermostability, and polyphenol production of perennial ryegrass. Crop Sci. 47(1), 261-267. Doi: 10.2135/cropsci2006.03.0171
Kavino, M., S. Harish, N. Kumar, D. Saravanakumar, and R. Samiyappan. 2010. Effect of chitinolytic PGPR on growth, yield and physiological attributes of banana (Musa sp) under field conditions. Appl. Soil Ecol. 45(2), 71-77. Doi: 10.1016/j.apsoil.2010.02.003
Kurabachew, H. and K. Wydra. 2014. Induction of systemic resistance and defense-related enzymes after elicitation of resistance by rhizobacteria and silicon application against Ralstonia solanacearum in tomato (Solanum lycopersicum). Crop Prot. 57, 1-7. Doi: 10.1016/j.cropro.2013.10.021
Lavakush, J. Yadav, J.P. Verma, D.K. Jaiswal, and A. Kumar. 2014. Evaluation of PGPR and different concentration of phosphorus level on plant growth, yield and nutrient content of rice (Oryza sativa). Ecol. Eng. 62, 123-128. Doi: 10.1016/j.ecoleng.2013.10.013
Lavinsky, A.O., K.C. Detmann, J.V. Reis, R.T. Ávila, M.L. Sanglard, L.F. Pereira, L.M.V.P. Sanglard, F.A. Rodrigues, W.L. Araújo, and F.M. DaMatta. 2016. Silicon improves rice grain yield and photosynthesis specifically when supplied during the reproductive growth stage. J. Plant Physiol. 206, 125-132. Doi: 10.1016/j.jplph.2016.09.010
Magalhães, J.E.S., E.A. Ferreira, M.C. Oliveira, G.A.M. Pereira, D.V. Silva, and J.B Santos. 2016. Effect of plant-biostimulant on cassava initial growth. Rev. Ceres 63(2), 208-213. Doi: 10.1590/0034-737X201663020012
Maghsoudi, K., Y. Emam, and M. Ashraf. 2016a. Foliar application of silicon at different growth stages alters growth and yield of selected wheat cultivars. J. Plant Nutr. 39(8), 1194-1203. Doi: 10.1080/01904167.2015.1115876
Maghsoudi, K., Y. Emam, and M. Pessarakli. 2016b. Effect of silicon on photosynthetic gas exchange, photosynthetic pigments, cell membrane stability and relative water content of different wheat cultivars under drought stress conditions. J. Plant Nutr. 39(7), 1001-1015. Doi: 10.1080/01904167.2015.1109108
Martínez, A.M. and D.G. Cayón. 2011. Dinámica del crecimiento y desarrollo del banano (Musa AAA Simmonds cvs. Gran Enano y Valery). Rev. Fac. Nac. Agron. Medellín 64(2), 6055-6064.
Mena-Violante, H.G. and V. Olalde-Portugal. 2007. Alteration of tomato fruit quality by root inoculation with plant growth-promoting rhizobacteria (PGPR): Bacillus subtilis BEB-13bs. Sci. Hortic. 113(1), 103-106. Doi: 10.1016/j.scienta.2007.01.031
Mia, M.A.B., Z.H. Shamsuddin, and M. Mahmood. 2010a. Use of plant growth promoting bacteria in banana: a new insight for sustainable banana production. Int. J. Agric. Biol. 12(3), 459-467.
Mia, M.A.B., Z.H. Shamsuddin, Z. Wahab, and M. Marziah. 2010b. Rhizobacteria as bioenhancer and biofertilizer for growth and yield of banana (Musa spp. cv. ‘Berangan’). Sci. Hortic. 126(2), 80-87. Doi: 10.1016/j.scienta.2010.06.005
Mohamed, H.I. and E.Z. Gomaa. 2012. Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescens on growth and pigment composition of radish plants (Raphanus sativus) under NaCl stress. Photosynthetica 50(2), 263-272. Doi: 10.1007/s11099-012-0032-8
Nazar, R., S. Umar, and N.A. Khan. 2015. Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress. Plant Signal. Behav. 10(3), e1003751. Doi: 10.1080/15592324.2014.1003751
Njukwe, E., A. Tenkouano, D. Amah, K. Sadik, M. Pérez, M. Nyine, and T. Dubois. 2007. Macro-propagation of banana and plantain: training manual. International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria.
Posmyk, M.M. and K. Szafrańska. 2016. Biostimulators: a new trend towards solving an old problem. Front. Plant Sci. 7, 748. Doi: 10.3389/fpls.2016.00748
Saa, S., A. Olivos-Del Rio, S. Castro, and P.H. Brown. 2015. Foliar application of microbial and plant based biostimulants increases growth and potassium uptake in almond (Prunus dulcis [Mill.] DA Webb). Front. Plant Sci. 6, 87. Doi: 10.3389/fpls.2015.00087
Saia, S., G. Colla, G. Raimondi, E. Di Stasio, M. Cardarelli, P. Bonini, P. Vitaglione, S. De Pascale, and Y. Rouphael. 2019. An endophytic fungi-based biostimulant modulated lettuce yield, physiological and functional quality responses to both moderate and severe water limitation. Sci. Hortic. 256, 108595. Doi: 10.1016/j.scienta.2019.108595
Song, A., P. Li, F. Fan, Z. Li, and Y. Liang. 2014. The effect of silicon on photosynthesis and expression of its relevant genes in rice (Oryza sativa L.) under high-zinc stress. PLoS ONE 9(11), e113782. Doi: 10.1371/journal.pone.0113782
Stefan, M., N. Munteanu, V. Stoleru, M. Mihasan, and L. Hritcu. 2013. Seed inoculation with plant growth promoting rhizobacteria enhances photosynthesis and yield of runner bean (Phaseolus coccineus L.). Sci. Hortic. 151, 22-29. Doi: 10.1016/j.scienta.2012.12.006
Turner, D.W. 1998. Ecophysiology of bananas: the generation and functioning of the leaf canopy. Acta Hortic. 490, 211-222. Doi: 10.17660/ActaHortic.1998.490.21
Ul Hassan, T. and A. Bano. 2015. The stimulatory effects of L-tryptophan and plant growth promoting rhizobacteria (PGPR) on soil health and physiology of wheat. J. Soil Sci. Plant Nutr. 15(1), 190-201. Doi: 10.4067/S0718-95162015005000016
Wang, Q., I.C. Dodd, A.A. Belimov, and F. Jiang. 2016. Rhizosphere bacteria containing 1-aminocyclopropane-1-carboxylate deaminase increase growth and photosynthesis of pea plants under salt stress by limiting Na+ accumulation. Funct. Plant Biol. 43(2), 161-172. Doi: 10.1071/FP15200
Yakhin, O.I., A.A. Lubyanov, I.A. Yakhin, and P.H. Brown. 2017. Biostimulants in plant science: a global perspective. Front. Plant Sci. 7, 2049. Doi: 10.3389/fpls.2016.02049
Xie, Z., F. Song, H. Xu, H. Shao, and R. Song. 2014. Effects of silicon on photosynthetic characteristics of maize (Zea mays L.) on alluvial soil. Sci. World J. 2014, 718716. Doi: 10.1155/2014/718716
Xu, H., Y. Lu, and Z. Xie. 2016. Effects of silicon on maize photosynthesis and grain yield in black soils. Emir. J. Food Agr. 28(11), 779-785. Doi: 10.9755/ejfa.2016-06-730
Zanetti, L.V., C.R.D. Milanez, V.N. Gama, M.A.G. Aguilar, C.A.S. Souza, E. Campostrini, T.M. Ferraz, and F.A.M.M.A. Figueiredo. 2016. Leaf application of silicon in young cacao plants subjected to water deficit. Pesq. Agropec. Bras. 51(3), 215-223. Doi: 10.1590/S0100-204X2016000300003
Zhang, H., X. Xie, M.S. Kim, D.A. Kornyeyev, S. Holaday, and P.W. Paré. 2008. Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in planta. Plant J. 56(2), 264-273. Doi: 10.1111/j.1365-313X.2008.03593.x