Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme

Abstract

The present study focused to evaluate the effect of a laccase from Aspergillus sp. in order to expose cellulose contained in both sugarcane (Saccharum officinarum, BCA) and Opuntia-ficus cactus (BOF) bagasses.  The kinetic parameters of the enzymatic activity were calculated for each of the materials, using the initial rates method at various bagasse:solution ratios (10 g:L to 50 g:L) and a total reaction time of 1 h. The assays presented a non-enzymatic removal of extractables by the buffer effect, under the evaluated reaction conditions. On the other hand, there was no dissolution of cellulose or hemicellulose, but there was lignin. Therefore, the results were analyzed, considering the Michaelis-Menten equation and using the disappearance rate of the g_lignin:L ratio as a basis. Through a Lineweaver-Burk linearization, the best fit of the values was obtained, where V_max =  14.9566 g_lignina:L and K_m = 40.2011 g_lignina:L/h for BCA assays (R = 0.9933) and V_max = 26.8300 g_lignina:L and K_m = 43.5575 g_lignina:L/h for BOF assays (R = 0.9972). Based on the above, although laccase presented a similar enzymatic affinity for both bagasses, a better catalytic efficiency is predicted towards lignin removal from BOF (0.6160).

Keywords

bagazo de caña, bagazo de cactáceas, lignina, celulosa, lacasa, cinética enzimática

Author Biography

Nubia Rosa Cardona López

Universidad Pedagógica y Tecnológica de Colombia, Procesos Ambientalmente Amigables, Avenida Central del Norte 39-115, Tunja, Colombia.

James Alberto Marín

Universidad de Antioquia, Grupo Procesos Químicos Industriales, Calle 67 # 53-108, Medellín, Antioquia.

Óscar Julio Medina Vargas

Universidad Pedagógica y Tecnológica de Colombia, Grupo de Investigación en Química y Tecnología de Alimentos, Avenida Central del Norte 39-115, Tunja, Colombia.

Luis Alberto Ríos

Universidad de Antioquia, Grupo Procesos Químicos Industriales, Calle 67 # 53-108, Medellín, Antioquia.

Gerardo Andrés Caicedo Pineda

Universidad Pedagógica y Tecnológica de Colombia, Procesos Ambientalmente Amigables, Avenida Central del Norte 39-115, Tunja, Colombia.

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