Skip to main navigation menu Skip to main content Skip to site footer

Evaluation of the ixodicide effect of botanical extracts on Rhipicephalus (Boophilus) microplus tick

Abstract

Ticks have negatively affected cattle production, causing great economic losses; due to the indiscriminate use of chemical products they have developed resistance, forcing the search for control alternatives, presenting an interest in investigating the acaricidal properties of plant species, the objective of the study was to determine the efficacy of the natural extract Phytolacca bogotensis and Alnus alcuminata in the control in vitro of the adult tick Rhipicephalus (Boophilus) microplus. The method used for the elaboration of the extracts of the analyzed plants was the hot extraction "Soxhlet", and the secondary metabolites were evaluated through qualitative tests. Adult ticks were exposed by the immersion technique to the pure extracts and dilutions. At 24, 48, 72 and 96h post-exposure, mortality was evaluated, taking a mortality of 60% as the minimum effective. The P. bogotensisextract showed effectiveness with the pure extract and the 1:2 dilution, and the pure extract of A. alcuminata did not exceed the minimum effective mortality rate. It was observed that the P. bogotensis extract showed greater efficacy in the control of the adult tick Rhipicephalus (Boophilus) microplus, constituting itself as a source of active principles that contribute to the discovery of ixodicides of natural origin.

Keywords

biologic control, ectoparasites, natural extracts, cattle

PDF (Español)

Author Biography

Carlos Eduardo Rodriguez Molano

Zootecnista, Universidad Nacional de Colombia, Especialista en Bioquímica, Investigación y Docencia, Magister en Ciencias Biológicas, Profesor Asociado Universidad Pedagógica y Tecnológica de Colombia. Coordinador Grupo de Investigacion en Bioquimica y Nutrición Animal


References

  1. Boulanger, N., Boyer, P., Talagrand-Reboul, E., & Hansmann, Y. (2019). Ticks and tick-borne diseases. Medecine et maladies infectieuses, vol. 49 no. 2 pp. 87-97. https://doi.org/10.1016/j.medmal.2019.01.007 DOI: https://doi.org/10.1016/j.medmal.2019.01.007
  2. García, D. D., Agatón, F. T., & Rosario-Cruz, R. (2016). Evaluación económica del control de garrapatas Rhipicephalus microplus en México/Economic evaluation of tick (Rhipicephalus microplus) control in Mexico. CIBA Revista Iberoamericana de las Ciencias Biológicas y Agropecuarias, vol. 5 no. 9 pp. 43-52. DOI: https://doi.org/10.23913/ciba.v5i9.49
  3. Polanco-Echeverry, D. N., & Ríos-Osorio, L. A. (2016). Biological and ecological aspects of hard ticks. Ciencia y Tecnología Agropecuaria, vol. 17 no. 1 pp. 81-95. DOI: https://doi.org/10.21930/rcta.vol17_num1_art:463
  4. Adenubi, O. T., Fasina, F. O., McGaw, L. J., Eloff, J. N., & Naidoo, V. (2016). Plant extracts to control ticks of veterinary and medical importance: A review. South African Journal of Botany, vol. 105 pp. 178-193. https://doi.org/10.1016/j.sajb.2016.03.010 DOI: https://doi.org/10.1016/j.sajb.2016.03.010
  5. FAO. (2004). Resistance Management and Integrated Parasite Control in Ruminants: Guidelines Module 1 - Ticks: Acaricide Resistance: Diagnosis, Management and Prevention. Food and Agriculture Organization, Animal Production and Health Division, Rome 53.
  6. Benavides-Montaño, J. A., Jaramillo-Cruz, C. A., & Mesa-Cobo, N. C. (2018). Garrapatas Ixodidae (Acari) en el valle del cauca, Colombia. Boletín Científico. Museo de Historia Natural, vol. 22 no. 1 pp. 131-150. https://doi.org/10.17151/bccm.2018.22.1.12 DOI: https://doi.org/10.17151/bccm.2018.22.1.12
  7. Weber, T. & Selzer, P. M. (2016). Isoxazolines: A Novel Chemotype Highly Effective on Ectoparasites. A Comprehensive Analysis of Parasite Biology: From Metabolism to Drug Discovery, pp. 105-119. https://doi.org/10.1002 / cmdc.201500516 DOI: https://doi.org/10.1002/9783527694082.ch4
  8. McNair, C. M. (2015). Ectoparasites of medical and veterinary importance: drug resistance and the need for alternative control methods. Journal of Pharmacy and Pharmacology, vol. 67 no. 3 pp. 351-363. https://doi.org/10.1111/jphp.12368 DOI: https://doi.org/10.1111/jphp.12368
  9. Pulido, N. J., Moyano, B. M. & Rodríguez, C. E. (2018). Evaluación de varias especies vegetales para inhibir la oviposición y controlar la proliferación de Rhipicephalus (Boophilus) microplus. Revista Cubana de Plantas Medicinales, vol. 23 no. 1.
  10. Rodríguez-Molano, C. E. & Pulido-Suárez, N. J. (2015). Efficacy of plant extracts on the adult tick Rhipicephalus (Boophilus) microplus and oviposition. Revista Cubana de Plantas Medicinales, vol. 20 no. 4 pp. 375-388.
  11. Rondina, R. V. & Coussio, J. D. (1989). Estudio fitoquímico de plantas medicinales argentinas. Revista de Investigaciones Agropecuarias. Biología y Producción Vegetal –INTA, vol. 6 no. 22 pp. 351-366.
  12. Cruz-Carrillo, A., Rodríguez-Molano, C. E., & Ortiz-López, C. (2011). Efecto insecticida in vitro del extracto etanólico de algunas plantas sobre la mosca adulta Haematobia irritans. Revista Cubana de Plantas Medicinales, vol. 16 no. 3 pp. 216-226.
  13. Jurado, B. (2010). Preparación de extractos y estudio fitoquímico de plantas biocidas. Primera edición. Universidad Del Perú. Lima, Perú pp. 1-26.
  14. Sanabria, G. A., López, S. I. & Gualdrón, R. (1997). Estudio fotoquímico preliminar y letalidad sobre Artemia salina de plantas colombianas. Rev. Colomb. Cienc. Quím. Farm vol. 26 no. 1 pp. 15-19.
  15. Klafke, G. M., Miller, R. J., Tidwell, J. P., Thomas, D. B., Sanchez, D., Feria Arroyo, T. P. y Pérez de León, A. A. (2019). Análisis de fusión de alta resolución (HRM) para la detección de SNP asociados con la resistencia a piretroides en la garrapata de la fiebre del ganado del sur, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). International Journal for Parasitology: Drugs and Drug Resistance, vol. 9 pp. 100-111. https://doi.org/10.1016/j.ijpddr.2019.03.001 DOI: https://doi.org/10.1016/j.ijpddr.2019.03.001
  16. Ruedisueli, F. L. & Manship, B. (2006). Tick identification key. University of Lincoln.
  17. Drummond R, Ernst S, Treviño J, Gladney W. (1973). Boophilus annulatus and Boophilus microplus: Laboratory test of insecticides. J Econ Entomol vol. 66 no. 1 pp. 130-133. DOI: https://doi.org/10.1093/jee/66.1.130
  18. Bravo, M. J., Coronado, A., & Henríquez, H. (2008). Eficacia in vitro del amitraz sobre poblaciones de Boophilus microplus provenientes de explotaciones lecheras del estado Lara, Venezuela. Zootecnia tropical, vol. 26 no. 1 pp. 35-40.
  19. FAO. (1993). Requisitos de efectividad biológica para los ixodicidas de uso en bovinos y método de prueba. Secretaria de Agricultura y Recursos Hidráulicos, México.
  20. Braga, A. G. S., Souza, K. F. A. D., Barbieri, F. D. S., Fernandes, C. D. F., Rocha, R. B., Vieira Junior, J. R., & Brito, L. G. (2018). Acaricidal activity of extracts from different structures of Piper tuberculatum against larvae and adults of Rhipicephalus microplus. Acta Amazonica, vol. 48 no. 1 pp. 57-62. https://doi.org/10.1590/1809-4392201700053 DOI: https://doi.org/10.1590/1809-4392201700053
  21. Galarraga, E., Amaro, J. M. A., Rojas, L., Offer, A. C. M., & Dubois, M. A. L. (2014). Triterpenos y saponinas triterpénicas de Phytolacca icosandra y Phytolacca rugosa. Ciencia, vol. 22 no. 221 pp. 53-66.
  22. Wu M. J., Wang L, Ding HY, Weng CY, Yen JH. (2014). Glossogyne tenuifolia Acts to Inhibit Inflammatory Mediator Production in a Macrophage Cell Line by Downregulating LPS-Induced NF-IκB. Journal of Biomedical Science vol. 11 no. 2 pp. 186-199. DOI: https://doi.org/10.1007/BF02256562
  23. Lopera, J. P., León, G., Guzmán, P. A., & Escobar, C. A. (2017). Efecto de los extractos vegetales de Jatropha curcas y Anonna muricata sobre teleoginas de la garrapata común del ganado Ripichephalus (Boophilus) microplus bajo condiciones de laboratorio. CES Medicina Veterinaria y Zootecnia, vol. 12 no. 1 pp. 21-32. DOI: https://doi.org/10.21615/cesmvz.12.1.2
  24. Jaramillo, H. D., González, R. A., Pedraza, C. N., Sierra, A. G., García, M. G. & Jara, A. R. (2020). Efecto acaricida de Momordica charantia, Megaskepasma erythrochlamys y Gliricidia sepium sobre Rhipicephalus microplus. Rev MVZ Córdoba. vol. 25 no. 1 pp. 1951. https://doi.org/10.21897/rmvz.1951 DOI: https://doi.org/10.21897/rmvz.1951
  25. Pochet, L., Frédérick, R., & Masereel, B. (2004). Coumarin and isocoumarin as serine protease inhibitors. Current pharmaceutical design, 10(30), 3781-3796. DOI: https://doi.org/10.2174/1381612043382684
  26. Fagotto, F. (1990). Yolk degradation in tick eggs: I. Occurrence of a cathepsin L‐like acid proteinase in yolk spheres. Archives of insect biochemistry and physiology, vol. 14 no. 4 pp. 217-235. https://doi.org/10.1002/arch.940140403 DOI: https://doi.org/10.1002/arch.940140403
  27. Amado, S. M. A., & Chocontá, A. C. (2016). Análisis fitoquímico de cinco especies nativas de las zonas rurales de Bogotá DC (Colombia). Boletín Semillas Ambientales, vol. 10 no. 1 pp. 15-20.
  28. Hüe, T., Cauquil, L., Fokou, J. B. H., Dongmo, P. M. J., Bakarnga, I., & Menut, C. (2015). Acaricidal activity of five essential oils of Ocimum species on Rhipicephalus (Boophilus) microplus larvae. Parasitology Research, vol. 114 no. 1 pp. 91-99. DOI: https://doi.org/10.1007/s00436-014-4164-6
  29. Dantas, A. C. S., Machado, D. M. R., Araujo, A. C., Oliveira-Junior, R. G., Lima-Saraiva, S. R. G., Ribeiro, L. A. A., ... & Horta, M. C. (2015). Acaricidal activity of extracts from the leaves and aerial parts of Neoglaziovia variegata (Bromeliaceae) on the cattle tick Rhipicephalus (Boophilus) microplus. Research in veterinary science, vol. 100 pp. 165-168. https://doi.org/10.1016/j.rvsc.2015.04.012 DOI: https://doi.org/10.1016/j.rvsc.2015.04.012
  30. Ghosh, S., Tiwari, S. S., Srivastava, S., Sharma, A. K., Nagar, G., Kumar, K. A., ... & Rawat, A. K. S. (2015). In vitro acaricidal properties of Semecarpus anacardium fruit and Datura stramonium leaf extracts against acaricide susceptible (IVRI-I line) and resistant (IVRI-V line) Rhipicephalus (Boophilus) microplus. Research in veterinary science, vol. 101 pp. 69-74. https://doi.org/10.1016/j.rvsc.2015.05.015 DOI: https://doi.org/10.1016/j.rvsc.2015.05.015
  31. González, G., Ojeda-Chi, M. M., Casanova L. F., Oros, I., Hernández, L. I., Piñeiro, Á. T., & Rodríguez, R. I. (2019.) Actividad acaricida de extractos etanólicos de tres genotipos de Leucaena spp. sobre Rhipicephalus microplus en condiciones in vitro. Revista mexicana de ciencias pecuarias, vol. 10 no. 3 pp. 692-704. DOI: https://doi.org/10.22319/rmcp.v10i3.4822
  32. Chang, F. R., Chen, C. Y., Wu, P. H., Kuo, R. Y., Chang, Y. C., & Wu, Y. C. (2000). New alkaloids from Annona purpurea. Journal of natural products, vol. 63 no. 6 pp. 746-748. https://doi.org/10.1021 / np990548n DOI: https://doi.org/10.1021/np990548n

Downloads

Download data is not yet available.

Most read articles by the same author(s)

Similar Articles

1 2 > >> 

You may also start an advanced similarity search for this article.