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Vol. 19 No. 1 (2025)
In Press / En prensa

Other species section

Modulating the PGPR activity of Lysinibacillus pinottii sp. nov. PB211 through plant sensitivity/resistance to exogenous auxins

https://doi.org/10.17584/rcch.2025v19i1.18010

Published 2025-01-01

  • Manuel Pantoja-Guerra
    • ,
  • Camilo A. Ramírez

Manuel Pantoja-Guerra
Universidad Popular del Cesar, Programa de Microbiología, Valledupar; INBACTER SAS, Departamento de Investigación y Desarrollo (I+D), La Estrella (Colombia)

Camilo A. Ramírez
Universidad de Antioquia, Instituto de Biología, Medellin (Colombia)

Modulating PGPR activity via plant auxin sensitivity. Photo: M. Pantoja-Guerra

Abstract

The effectiveness of plant growth-promoting rhizobacteria (PGPR) strains indole-3-acetic acid (IAA) producers may be influenced by the plant's resistance to auxins. In this work, the impact of auxin resistance on the PGPR activity of Lysinibacillus pinottii sp. nov. PB211 was investigated. PB211 produced an average of 32 μg mL-1 of indole-3-acetic acid (IAA). Genetic evidence indicated that PB211 utilizes the indole pyruvic acid pathway for IAA synthesis. Regards to the response of plant models to IAA treatment, eudicot models (cucumber and bean) exhibited higher sensitivity to IAA compared to monocot models (corn and brachiaria). Monocots required higher IAA concentrations to elicit phenotypic changes in root architecture. The resistance/sensitivity of plants to exogenous auxins was found to modulate the PGPR activity of PB211. Inoculation with PB211 at varying concentrations resulted in differential effects on plant models. Eudicots displayed significant PGPR activity from lower inoculum concentrations, whereas monocots required higher inoculum concentrations to exhibit a similar consistent effect. The PB211 effect also was evaluated on Arabidopsis thaliana wild-type (col-0 “auxin-sensible”) and mutant (aux1-7/axr4-2 “auxin-resistant”) plants. PB211 had a “bell-shaped” effect on wild-type plants response, a typical response of auxin-activity, so, the PGPR effect decreased at the highest inoculum concentration. Conversely, the mutant plants exhibited increased PGPR activity with higher inoculum concentrations, compensating for their auxin-deficient phenotype. These findings suggest that the resistance/sensitivity of plants to exogenous auxins influences the effect of auxin-producer PGPRs. These relationships could facilitate the development and application of more effective biological inoculants for agriculture.

Keywords

Monocots, Eudicots, Indole-3-acetic acid, Plant growth promotion, Biofertilizers, Biostimulants

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