Parasitism of Trichogramma and predation of Chrysoperla on Compsus viridivittatus eggs, a Vitis vinifera pest, under laboratory conditions

Main Article Content


Diego Alonso Pinzón-Hamón
John Wilson Martínez-Osorio
Mayerly Alejandra Castro-López


The parasitic potential of Trichogramma exiguum and Trichogramma pretiosum and the predatory effect of Chrysoperla carnea were evaluated on Compsus viridivittatus eggs, an insect pest in grape fields in Boyaca, Colombia. The T. exiguum and T. pretiosum tests used 240 eggs groups exposed to 0, 15, 30 and 45 parasitoids densities. In the C. carnea test, a second instar larva was placed with prey densities of 80, 160 and 240 C. viridivittatus eggs. A completely randomized design was used under laboratory conditions (18±2°C and 60±5% RH). In the trials with T. exiguum and T. pretiosum, the highest percentages of parasitism were obtained with the 45 parasitoid density (69.4% and 82.5%, respectively). It was established that T. pretiosum was more effective in the parasitism of C. viridivittatus than T. exiguum, resulting in larvae emergence under 13% and parasitism over 70%. The test with C. carnea showed that the highest consumption percentage (85.8%) was obtained with the 80 C. viridivittatus egg density. There was no emergence of parasitoids from the eggs parasitized by T. exiguum and T. pretiosum, probably because of the environmental conditions and/or C. viridivittatus host intrinsic characteristics. The tested entomophagous species showed over 50% C. viridivittatus egg mortality, suggesting that they can be used in future field tests in grape vine crops in order to observe their potential in C. viridivittatus biological control programs in Boyaca.


Article Details


The copyright of the articles and illustrations are the property of the Revista Colombiana de Ciencias Hortícolas. The editors authorize the use of the contents under the Creative Commons license Attribution-Noncommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). The correct citation of the content must explicitly register the name of the journal, name (s) of the author (s), year, title of the article, volume, number, page of the article and DOI. Written permission is required from publishers to publish more than a short summary of the text or figures.


Boina, D.R., E.O. Onagbola, M. Salyani, and L.L. Stelinski. 2009. Antifeedant and sublethal effects of imidacloprid on Asian citrus psyllid, Diaphorina citri. Pest Manag. Sci. 65, 870-877. Doi: 10.1002/ps.1767

Boina, D.R. and J.R. Bloomquist. 2015. Chemical control of the Asian Citrus Psyllid and of Huanglongbing disease in citrus. Pest Manag. Sci. 71, 808-823. Doi: 10.1002/ps.3957

Castle, J.S., F.J. Byrne, J.L. Bi, and N.C. Toscano. 2005. Spatial and temporal distribution of imidacloprid and thiamethoxam in citrus and impact on Homalodisca coagulate populations. Pest Manag. Sci. 61, 75-84. Doi: 10.1002/ps.949

Chen, X.D. and L.L. Stelinski. 2017a. Rapid detection of insecticide resistance in Diaphorina citri (Hemiptera: Liviidae) populations, using a bottle bioassay. Fla. Entomol. 100, 124-133. Doi: 10.1653/024.100.0118

Chen, X.D. and L.L. Stelinski. 2017b. Resistance management for Asian Citrus Psyllid, Diaphorina citri Kuwayama, in Florida. Insects 8, 103. Doi: 10.3390/insects8030103

Cocco, A., and M.A. Hoy, M.A. 2008. Toxicity of organosilicone adjuvants and selected pesticides to the Asian citrus psyllid (Hemiptera: Psyllidae) and its parasitoid Tamarixia radiata (Hymenoptera: Eulophidae). Fla. Entomol. 9, 610-620. Doi: 10.1653/0015-4040-91.4.610

Davies, T.G.E., L.M. Field, P.N.R. Usherwood, and M.S. Williamson. 2007. DDT, pyrethrins, pyrethroids and insect sodium channels. IUBMB life 59, 151-162. Doi: 10.1080/15216540701352042

Ebratt-Ravelo, E.E., L.T. Rubio-González, V.A. Costa, A.P. Castro-Ávila, E.M. Zambrano-Gómez, and J.E. Ángel-Díaz. 2011. Diaphorina citri (Kuwayama, 1907) and Tamarixia radiata (Waterson, 1922) in citrus crops of Cundinamarca, Colombia. Agron. Colomb. 29, 487-493.

Elbert, A., M. Haas, B. Springer, W. Thielert, and R. Nauen. 2008. Applied aspects of neonicotinoid uses in crop protection. Pest Manag. Sci. 64, 1099-1105. Doi: 10.1002/ps.1616

Gatineau, F., F. Bonnot, T.T.H. Yen, N.D. Tuyen, and N.T.N. Truc. 2010. Effects of imidacloprid and fenobucarb on the dynamics of the psyllid Diaphorina citri Kuwayama and on the incidence of Candidatus Liberibacter asiaticus. Fruits 65, 209-220. Doi: 10.1051/fruits/2010017

Gómez, K.A. and A.A. Gómez AA. 1984. Statistical procedures for agricultural research. John Wiley & Sons, Toronto, Canada.

Henderson, C.F. and E.W. Tilton. 1955. Tests with acaricides against the brown wheat mite. J. Econ. Entomol. 48, 157-161. Doi: 10.1093/jee/48.2.157

Hernández-Fuentes, L.M., M.A. Urias-López, J.I. López-Arroyo, R. Gómez-Jaimes, and N. Bautista-Martínez. 2012. Control químico de Diaphorina citri Kuwayama (Hemíptera: Psyllidae) en Lima Persa Citrus latifolia Tanaka. Rev. Mex. Cienc. Agríc. 3, 427-439.

Hoffmann, E.J., S.M. Middleton, and J.C. Wise. 2008. Ovicidal activity of organophosphate, oxadiazine, neonicotinoid and insect growth regulator chemistries on northern strain plum curculio, Conotrachelus nenuphar. J. Insect Sci. 8, 1-6. Doi: 10.1673/031.008.2901

ICA (Instituto Colombiano de Agropecuario). 2015. Resolución 2390 de 2015, Por medio de la cual se declara el estado de emergencia fitosanitaria en el territorio nacional por la presencia de adultos de Diaphorina citri infectados con la bacteria de la enfermedad del HLB de los cítricos. Bogotá.

ICA (Instituto Colombiano de Agropecuario). 2017. Resolución número 00007109 de 2017, por medio de la cual se declara el estado de emergencia fitosanitaria en el territorio nacional por la presencia de la enfermedad conocida como Huanglongbing (HLB) de los cítricos. Bogotá.

Langdon, K.W. and M.E. Rogers. 2017. Neonicotinoid-induced mortality of Diaphorina citri (Hemiptera: Liviidae) is affected by route of exposure. J. Econ. Entomol. 110, 2229-2234. Doi: 10.1093/jee/tox231

Maienfisch, P., M. Angst, F. Brand, W. Fischer, D. Hofer, H. Kayser, W. Kobel, A. Rindlisbacher, R. Senn, A. Steinemann, and H. Widmer 2001. Chemistry and biology of thiamethoxam: a second-generation neonicotinoid. Pest Manag. Sci. 57, 906-913. Doi: 10.1002/ps.365

Pardo, S., A.M. Martínez, J.I. Figueroa, J.M. Chavarrieta, E. Viñuela, Á. Rebollar-Alviter, M.A. Miranda, J. Valle, and S. Pineda. 2017. Insecticide resistance of adults and nymphs of Asian Citrus Psyllid populations from Apatzingán Valley, Mexico. Pest Manag. Sci. 74, 135-140. Doi: 10.1002/ps.4669

Qureshi, J.A., B.C. Kostyk, and P.A. Stansly. 2009. Control of Diaphorina citri (Hemiptera: Psyllidae) with foliar and soil-applied insecticides. P. Fl. St. Hortic. Soc. 122, 189-193.

Qureshi, J.A., B.C. Kostyk, and P.A. Stansly. 2014. Insecticidal suppression of Asian Citrus Psyllid Diaphorina citri (Hemiptera: Liviidae) vector of huanglongbing pathogens. PloS One 9, e112331. Doi: 10.1371/journal.pone.0112331

Ruiz-Galván, I., N. Bautista-Martínez, H. Sánchez-Arroyo, and F.A. Valenzuela-Escoboza. 2015. Control químico de Diaphorina citri (Kuwayama) (Hemiptera: Liviidae) en lima persa. Acta Zool. Mex. 31, 41-47. Doi: 10.21829/azm.2015.311503

Santivañez, T., G. Mora Aguilera, G. Diaz Pinilla, J.I. López Arrollo, and P. Vernal Hurtado. 2013. Citrus. Marco estratégico para la gestión regional del Huanglongbing en América Latina y el Caribe. FAO, Santiago.

Sétamou, M., D. Flores, J.V. French, and D.G. Hall. 2008. Dispersion patterns and sampling plans for Diaphorina citri (Hemiptera: Psyllidae) in citrus. J. Econ. Entomol. 101, 1478-1487. Doi: 10.1093/jee/101.4.1478

Sétamou, M., D. Rodriguez, R. Saldana, G. Schwarzlose, D. Palrang, and S.D. Nelson. 2010. Efficacy and uptake of soil-applied imidacloprid in the control of Asian Citrus Psyllid and a citrus leafminer, two foliar-feeding citrus pests. J. Econ. Entomol. 103, 1711-1719. Doi: 10.1603/EC09371

Smith, E.H. and E.H. Salkeld. 1966. The use and action of ovicides. Annu. Rev. Entomol. 11, 331-368. Doi: 10.1146/annurev.en.11.010166.001555

Stansly, P.A., J.A. Qureshi, and B.C. Kostyk. 2015. Evaluation of organophosphate and pyrethroid insecticides for control of Asian Citrus Psyllid and citrus leafminer on oranges, summer, 2014. Arthropod Manag. Tests 40, 1-2. Doi: 10.1093/amt/tsv045

Tiwari, S., R.S. Mann, M.E. Rogers, and L.L. Stelinski. 2011. Insecticide resistance in field populations of Asian citrus psyllid in Florida. Pest Manag. Sci. 67, 1258-1268. Doi: 10.1002/ps.2181

Yamamoto, P.T., M.R. Fellipe, A.L. Sanches, J.H. Coelho, L.F. Garbim, and N.L. Ximenes. 2009. Eficácia de insecticidas para o manejo de Diaphorina citri Kuwayama (Hemiptera: Psyllidae) em citros. BioAssay 4, 1-9. Doi: 10.14295/BA.v4.0.21


Download data is not yet available.