Análisis sensorial del licor de cacao (Theobroma cacao L.) en cultivares de diferentes orígenes cultivados en el trópico colombiano
Resumen
Se evaluó el perfil sensorial de 16 cultivares de cacao de diferentes orígenes de las zonas productoras de los departamentos de Arauca, Huila y Santander en Colombia. Este estudio utilizó el Laboratorio de Calidad Integral de Cacao del Instituto Nacional de Investigaciones Agropecuarias (INIAP) en la Estación Experimental Tropical Pichilingue (Quevedo-Ecuador), con un panel de cuatro evaluadores que analizaron los atributos de sabor típicos del licor de cacao como: cacao, ácido, amargo, astringente, frutal, floral, nuez, dulce y verde/crudo. Los valores medios de las calificaciones asignadas por los evaluadores mostraron que el perfil sensorial varió considerablemente entre genotipos y localidades en estudio. Algunos genotipos presentaron perfiles de sabor más complejos que el control, CCN 51. Los genotipos introducidos EET 8, ICS 1, ICS 39 e ICS 60, presentaron baja intensidad en los atributos evaluados, mientras que la mayoría de los materiales regionales seleccionados presentaron mayor intensidad de notas frutales, cacao y nuez, especialmente FLE 3, FSV 41, FEC 2 y FEAR 5. Los perfiles sensoriales demostraron el potencial del cacao colombiano con su fino aroma.
Palabras clave
Calidad, Perfil sensorial, Sabores, Aromas
Citas
- Afoakwa, E.O., A. Paterson, M. Fowler, and A. Ryan. 2008. Flavor formation and character in cocoa and chocolate: a critical review. Crit. Rev. Food Sci. Nutr. 48(9), 840-857. Doi: https://doi.org/10.1080/10408390701719272
- Afoakwa, E.O., J. Quao, F.S. Takrama, A.S. Budu, and F.K. Saalia. 2012. Changes in total polyphenols, o-diphenols and anthocyanin concentrations during fermentation of pulp pre-conditioned cocoa (Theobroma cacao) beans. Int. Food Res. J. 19(3), 1071-1077.
- Argout, X., O. Fouet, P. Wincker, K. Gramacho, T. Legavre, X. Sabau, A.M. Risterucci, C. Silva, J. Cascardo, M. Allegre, D. Kuhn, J. Verica, B. Courtois, G. Loor, R. Babin, O. Sounigo, M. Ducamp, M.J. Guiltinan, M. Ruiz, L. Alemanno, R. Machado, W. Phillips, R. Schnell, M. Gilmour, E. Rosenquist, D. Butler, S. Maximova, and C. Lanaud. 2008. Towards the understanding of the cocoa transcriptome: production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions. BMC Genom. 9, 512. Doi: https://doi.org/10.1186/1471-2164-9-512
- Bastos, V.S., T.M. Uekane, N.A. Bello, C.M. Rezende, V.M.F. Paschoalin, and E.M. Del Aguila. 2019. Dynamics of volatile compounds in TSH 565 cocoa clone fermentation and their role on chocolate flavor in Southeast Brazil. J. Food Sci. Technol. 56, 2874-2887. Doi: https://doi.org/10.1007/s13197-019-03736-3
- Batista, N.N., C.L. Ramos, D.R. Dias, A.C.M. Pinheiro, and R.F. Schwan. 2016. The impact of yeast starter cultures on the microbial communities and volatile compounds in cocoa fermentation and the resulting sensory attributes of chocolate. J. Food Sci. Technol. 53, 1101-1110. Doi: https://doi.org/10.1007/s13197-015-2132-5
- Bekele, F.L., I. Bekele, D.R. Butler, and G.G. Bidaisee. 2006. Patterns of morphological variation in a sample of cacao (Theobroma cacao L.) germplasm from the International Cocoa Genebank, Trinidad. Genet. Resour. Crop Evol. 53, 933-948. Doi: https://doi.org/10.1007/s10722-004-6692-x
- Bertoldi, D., A. Barbero, F. Camin, A. Caligiani, and R. Larcher. 2016. Multielemental fingerprinting and geographic traceability of Theobroma cacao beans and cocoa products. Food Control 65, 46-53. Doi: https://doi.org/10.1016/j.foodcont.2016.01.013
- Boza, E.J., J.C. Motamayor, F.M. Amores, S. Cedeño-Amador, C.L. Tondo, D.S. Livingstone III, and O.A. Gutiérrez. 2014. Genetic characterization of the cacao cultivar CCN 51: its impact and significance on global cacao improvement and production. J. Am. Soc. Hort. Sci. 139(2), 219-229. Doi: https://doi.org/10.21273/JASHS.139.2.219
- Cambrai, A., C. Marcic, S. Morville, P.S. Houer, F. Bindler, and E. Marchioni. 2010. Differentiation of chocolates according to the cocoa’s geographical origin using chemometrics. J. Agric. Food Chem. 58(3), 1478-1483. Doi: https://doi.org/10.1021/jf903471e
- Carrillo, L.C., J. Londoño-Londoño, and A. Gil. 2014. Comparison of polyphenol, methylxanthines and antioxidant activity in Theobroma cacao beans from different cocoa-growing areas in Colombia. Food Res. Int. 60, 273-280. Doi: https://doi.org/10.1016/j.foodres.2013.06.019
- Cevallos-Cevallos, J.M., L. Gysel, M.G. Maridueña-Zavala, and M.J. Molina-Miranda. 2018. Time-related changes in volatile compounds during fermentation of bulk and fine-flavor cocoa (Theobroma cacao) beans. J. Food Qual. 2018, 1758381. Doi: https://doi.org/10.1155/2018/1758381
- CoEx, Cacao of Excellence Programme. 2015. International cocoa awards results of the 2015 edition. In: www.cacaoofexcellence.org; consulted: May, 2023.
- Counet, C., C. Ouwerx, D. Rosoux, and S. Collin. 2004. Relationship between procyanidin and flavor contents of cocoa liquors from different origins. J. Agric. Food Chem. 52(20), 6243-6249. Doi: https://doi.org/10.1021/jf040105b
- Di Carro, M., F. Ardini, and E. Magi. 2015. Multivariate optimization of headspace solid-phase microextraction followed by gas chromatography–mass spectrometry for the determination of methylpyrazines in cocoa liquors. Microchem. J. 121, 172-177. Doi: https://doi.org/10.1016/j.microc.2015.03.006
- FAO. 2022. Statics platform of the Food and Agriculture Organization FAOSTAT: Crops and livestock products. In: http://www.fao.org/faostat/es/#home ; consulted: June, 2023.
- Frauendorfer, F. and P. Schieberle. 2008. Changes in key aroma compounds of criollo cocoa beans during roasting. J. Agric. Food Chem. 56(21), 10244-10251. Doi: https://doi.org/10.1021/jf802098f
- González, Y., E. Pérez, and C. Palomino. 2012. Factores que inciden en la calidad sensorial del chocolate. Actual. Nutr. 13(4), 314-331.
- ICCO, International Cocoa Organization. 2022. What is Fine Flavour Cocoa? In:: https://www.icco.org/fine-or-flavor-cocoa/; consulted: May, 2023.
- ICONTEC, Instituto Colombiano de Normas Técnicas y Certificación. 2021. NTC 3929: Análisis sensorial. Metodología. Métodos del perfil del sabor. Segunda actualización. Bogota.
- ISO, International Organization for Standardization. 2012. ISO 8586, sensory analysis - General guidelines for the selection, training and monitoring of selected assessors and expert sensory assessors. In: https://www.iso.org/standard/45352.html; consulted: May, 2023.
- Jiménez, J.C., F.M. Amores, C. Nicklin, D. Rodríguez, F. Zambrano, M. Bolaños, V. Reynel, A. Dueñas, and P. Cedeño. 2011. Micro fermentación y análisis sensorial para la selección de árboles superiores de cacao. Bol. Tec. 140. INIAP, Quevedo, Ecuador.
- Johnson, E.S., F.L. Bekele, S.J. Brown, Q. Song, D. Zhang, L.W. Meinhardt, and R.J. Schnell. 2009. Population structure and genetic diversity of the Trinitario cacao (Theobroma cacao L.) from Trinidad and Tobago. Crop Sci. 49(2), 564-572. Doi: https://doi.org/10.2135/cropsci2008.03.0128
- Luna, F., D. Crouzillat, L. Cirou, and P. Bucheli. 2002. Chemical composition and flavor of Ecuadorian cocoa liquor. J. Agric. Food Chem. 50(12), 3527-3532. Doi: https://doi.org/10.1021/jf0116597
- Luykx, D.M.A.M. and S.M. van Ruth. 2008. An overview of analytical methods for determining the geographical origin of food products. Food Chem. 107(2), 897-911. Doi: https://doi.org/10.1016/j.foodchem.2007.09.038
- Menezes, A.G.T., N.N. Batista, C.L. Ramos, A.R.A. Silva, P. Efraim, A.C.M. Pinheiro, and R.F. Schwan. 2016. Investigation of chocolate produced from four different Brazilian varieties of cocoa (Theobroma cacao L.) inoculated with Saccharomyces cerevisiae. Food Res. Int. 81, 83-90. Doi: https://doi.org/10.1016/j.foodres.2015.12.036
- Moreira, I.M.V., M.G.C.P. Miguel, W.F. Duarte, D.R. Dias, and R.F. Schwan. 2013. Microbial succession and the dynamics of metabolites and sugars during the fermentation of three different cocoa (Theobroma cacao L.) hybrids. Food Res. Int. 54(1), 9-17. Doi: https://doi.org/10.1016/j.foodres.2013.06.001
- Ordoñez, E.S., Y. Quispe, and L.F. García. 2020. Cuantificación de fenoles, antocianinas y caracterización sensorial de nibs y licor de cinco variedades de cacao, en dos sistemas de fermentación. Sci. Agropec. 11(4), 473-481. Doi: https://doi.org/10.17268/sci.agropecu.2020.04.02
- Owusu, M., M.A. Petersen, and H. Heimdal. 2012. Effect of fermentation method, roasting and conching conditions on the aroma volatiles of dark chocolate. J. Food Proces. Preserv. 36(5), 446-456. Doi: https://doi.org/10.1111/j.1745-4549.2011.00602.x
- Palacios, A.M., J.N. Quevedo, and I. Rodriguez. 2021. FETENSECA: alternativa para mejorar la calidad sensorial del cacao (Theobroma cacao L.) cultivar CCN-51. Manglar 18(4), 411-417. Doi: https://doi.org/10.17268/manglar.2021.053
- Perea, J.A., N. Martínez, F. Aránzazu, and T. Cadena. 2017. Características de la calidad del cacao de Colombia: catálogo de 26 cultivares. Universidad Industrial de Santander; Fedecacao, Bucaramanga, Colombia.
- Pino, J.A., L. Llera, and E. Roncal. 2011. Caracterización del perfil de compuestos volátiles de licores de cacao cubanos. Instituto de Investigaciones para la Industria Alimentaria, La Habana.
- Porras, L.D., J.D. Torres, M.A. Gil, and O.L. Martínez. 2019. Effect of the solar drying process on the sensory and chemical quality of cocoa (Theobroma cacao L.) cultivated in Antioquia, Colombia. Food Res. Int. 115, 259-267. Doi: https://doi.org/10.1016/j.foodres.2018.08.084
- Ramos, C.L., D.R. Dias, M.G.C.P Miguel, and R.F. Schwan. 2014. Impact of different cocoa hybrids (Theobroma cacao L.) and S. cerevisiae UFLA CA11 inoculation on microbial communities and volatile compounds of cocoa fermentation. Food Res. Int. 64, 908-918. Doi: https://doi.org/10.1016/j.foodres.2014.08.033
- Ramos, G., N. González, A. Zambrano, and A. Gómez. 2013. Smells and flavors of Venezuelan cocoa (Theobroma cacao L.) obtained using a trained tasting panel. Rev. Cient. UDO Agric. 13(1), 114-127.
- Ríos, F., A. Ruiz, J. Lecaro, and C. Rehpani. 2017. Estrategias país para la oferta de cacaos especiales. Políticas e iniciativas privadas exitosas en el Perú, Ecuador, Colombia y República Dominicana. Fundación Swisscontact Colombia, Bogota.
- Rodríguez-Campos, J., H.B. Escalona-Buendía, I. Orozco-Ávila, E. Lugo-Cervantes, and M.E. Jaramillo-Flores. 2011. Dynamics of volatile and non-volatile compounds in cocoa (Theobroma cacao L.) during fermentation and drying processes using principal components analysis. Food Res. Int. 44(1), 250-258. Doi: https://doi.org/10.1016/j.foodres.2010.10.028
- Sim, S.Y.J., J.W. Ng, W.K. Ng, C.G. Forde, and C.J. Henry. 2016. Plant polyphenols to enhance the nutritional and sensory properties of chocolates. Food Chem. 200, 46-54. Doi: https://doi.org/10.1016/j.foodchem.2015.12.092
- Streule, S., S.F. Leischtfeld, M. Galler, and S.W. Schwenninger. 2022. Monitoring of cocoa post-harvest process practices on a small-farm level at five locations in Ecuador. Heliyon 8(6), e09628. Doi: https://doi.org/10.1016/j.heliyon.2022.e09628
- Sukha, D.A., D.R. Butler, P. Umaharan, and E. Boult. 2008. The use of an optimised organoleptic assessment protocol to describe and quantify different flavour attributes of cocoa liquors made from Ghana and Trinitario beans. Eur. Food Res. Technol. 226, 405-413. Doi: https://doi.org/10.1007/s00217-006-0551-2
- Vázquez-Ovando, A., F. Molina-Freaner, J. Nuñez-Farfán, and M. Salvador-Figueroa. 2012. Potencial de los marcadores moleculares para el rescate de individuos de Theobroma cacao L. de alta calidad. BioTecnología 16(1), 36-56.