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Vol. 12 No. 2 (2018)

Fruits section

Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

https://doi.org/10.17584/rcch.2018v12i2.7226

Published 2018-05-04

  • Paulo Mauricio Centenaro Bueno
    • ,
  • Luiz Antonio Biasi
    • ,
  • Mauro Brasil Dias Tofanelli

Paulo Mauricio Centenaro Bueno
Instituto Federal do Paraná, Campus Palmas, Palmas-PR; Universidade Federal do Paraná, Departamento de Fitotecnia e Fitossanitarismo, Curitiba-PR

Luiz Antonio Biasi
Universidade Federal do Paraná, Departamento de Fitotecnia e Fitossanitarismo, Curitiba-PR

Mauro Brasil Dias Tofanelli
Universidade Federal do Paraná, Departamento de Fitotecnia e Fitossanitarismo, Curitiba-PR

Abstract

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

Keywords

Native species, tissue culture, rooting, microcutting, explant, growth regulators

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Author Biography

Paulo Mauricio Centenaro Bueno

Agronomist, PhD in Plant Sciences.

Professor at IFPR, teaching plant phisiology, plant breeding, fruitculture and anual crops.

Luiz Antonio Biasi

Departamento de Fitotecnia e Fitossanitarismo

Setor de Ciências Agrárias

Mauro Brasil Dias Tofanelli

Departamento de Fitotecnia e Fitossanitarismo

Setor de Ciências Agrárias

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