Efecto letal y repelente del monoterpeno geraniol sobre ninfas de Triatoma infestans susceptibles y resistentes a deltametrina

Carla Rodas; Martin Daniele; Naomi Simon Difiori; Gustavo Marin; Guillermo Schinella; Roger Iván Rodríguez-Vivas; Martín Dadé

doi:10.19053/01228420.v20.n1.2023.15045

Authors

Carla Rodas Universidad Nacional Arturo Jauretche (CICPBA), Instituto de Ciencias de la Salud, Florencio Varela
Martin Daniele Universidad Nacional de Río Negro, Sede Alto Valle y Valle Medio, Escuela de Veterinaria y Produccion Agroindustrial, Choele Choel; Universidad de Ciencias Empresariales y Sociales (UCES), Carrera de Veterinaria, Cañuelas, Buenos Aires https://orcid.org/0000-0001-8744-2351
Naomi Simon Difiori Universidad Nacional de Río Negro, Sede Alto Valle y Valle Medio, Escuela de Veterinaria y Produccion Agroindustrial, Choele Choel
Gustavo Marin Universidad Nacional de La Plata, Facultad de Ciencias Médicas, Cátedra de Farmacología Básica, La Plata https://orcid.org/0000-0002-6380-6453
Guillermo Schinella Universidad Nacional Arturo Jauretche (CICPBA), Instituto de Ciencias de la Salud, Florencio Varela; Universidad Nacional de La Plata, Facultad de Ciencias Médicas, Cátedra de Farmacología Básica, La Plata https://orcid.org/0000-0001-9541-9688
Roger Iván Rodríguez-Vivas Universidad Autónoma de Yucatán, Facultad de Medicina Veterinaria and Zootecnia, Mérida https://orcid.org/0000-0002-3340-8059
Martín Dadé Universidad Nacional Arturo Jauretche (CICPBA), Instituto de Ciencias de la Salud, Florencio Varela; Universidad Nacional de Río Negro, Sede Alto Valle y Valle Medio, Escuela de Veterinaria y Produccion Agroindustrial, Choele Choel; Universidad de Ciencias Empresariales y Sociales (UCES), Carrera de Veterinaria, Cañuelas, Buenos Aires; Universidad Nacional de La Plata, Facultad de Ciencias Médicas, Cátedra de Farmacología Básica, La Plata

DOI:

https://doi.org/10.19053/01228420.v20.n1.2023.15045

Keywords:

Chagas disease, Insecticide resistance, Pyrethroid, Bioinsecticides, Potentiation

Abstract

Triatoma infestans is the main vector of the Trypanosoma cruzi parasite, the etiological agent of chagas disease. The most effective strategy for the control of T. infestans are pyrethroid-type insecticides. However, the presence of specimens of T. infestans resistant to pyrethroids now sparks the need to seek new alternatives for their control. Bioinsecticides are currently positioned as a novel alternative, less aggressive for the environment and less costly compared to traditional synthetic insecticides. Geraniol is a monoterpene that has been shown to have insecticidal and repellent activity on insects. The objectives of this work were to determine and compare the lethal and repellent activity of geraniol alone and in combination with the pyrethroid insecticide deltamethrin and the insect repellent N,N-Diethyl-meta-toluamide (DEET). Geraniol was shown to have similar lethal activity in pyrethroid-susceptible and -resistant nymphs (resistance ratio of 0.8). When the two insecticides were combined, geraniol showed a synergistic effect on the lethality of deltamethrin. Regarding their repellent activity, at low concentrations, geraniol was less potent than DEET; however, when both molecules were combined, the presence of this monoterpene increased the repellency capacity of DEET to 100%. It is concluded that geraniol has lethal activity on T. infestans nymphs susceptible and resistant to pyrethroids and has a synergistic effect on the lethality of deltamethrin. Likewise, geraniol increased the repellency capacity of DEET on T. infestans.

Downloads

Download data is not yet available.

References

Barnard, D. R., & Xue, R.-D. (2004). Laboratory evaluation of mosquito repellents against Aedes albopictus, Culex nigripalpus, and Ochierotatus triseriatus (Diptera: Culicidae). Journal of Medical Entomology, 41(4), 726–730. https://doi.org/10.1603/0022-2585-41.4.726

Busvine, J. R. (1957). A critical review of the techniques for testing insecticides. Commonwealth Institute of Entomology.

Chapman, R. F. (2013). The insects: Structure and function (5th ed). Cambridge University Press,

Chen, W., & Viljoen, A. M. (2010). — A review of a commercially important fragrance material. South African Journal of Botany, 76(4), 643–651. https://doi.org/10.1016/j.sajb.2010.05.008

Choochote, W., Chaithong, U., Kamsuk, K., Jitpakdi, A., Tippawangkosol, P., Tuetun, B., Champakaew, D., & Pitasawat, B. (2007). Repellent activity of selected essential oils against Aedes aegypti. Fitoterapia, 78(5), 359–364. https://doi.org/10.1016/j.fitote.2007.02.006

Chou, T.-C. (2006). Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies. Pharmacological Reviews, 58(3), 621–681. https://doi.org/10.1124/pr.58.3.10

Dadé, M., Zeinsteger, P., Bozzolo, F., & Mestorino, N. (2018). Repellent and lethal activities of extracts from fruits of chinaberry (Melia azedarach L., Meliaceae) against Triatoma infestans. Frontiers in Veterinary Science, 5, 158. https://doi.org/10.3389/fvets.2018.00158

Dadé, M. M., Daniele, M. R., Machicote, M., Errecalde, J. O., & Rodriguez-Vivas, R. I. (2020). First report of the lethal activity and synergism between deltamethrin, amitraz and piperonyl butoxide against susceptible and pyrethroid-resistant nymphs of Triatoma infestans. Experimental Parasitology, 218, 107986. https://doi.org/10.1016/j.exppara.2020.107986

De la Vega, G. J. (2016). Bases fisiológicas de la distribución de triatominos vectores de la enfermedad de Chagas [tesis de doctorado]. Universidad de Buenos Aires. https://bibliotecadigital.exactas.uba.ar/download/tesis/tesis_n5973_DelaVega.pdf

Deletre, E., Martin, T., Duménil, C., & Chandre, F. (2019). Insecticide resistance modifies mosquito response to DEET and natural repellents. Parasites & Vectors, 12(1), 89. https://doi.org/10.1186/s13071-019-3343-9

Gaire, S., Lewis, C. D., Booth, W., Scharf, M. E., Zheng, W., Ginzel, M. D., & Gondhalekar, A. D. (2020). Bed bugs, Cimex lectularius L., exhibiting metabolic and target site deltamethrin resistance are susceptible to plant essential oils. Pesticide Biochemistry and Physiology, 169, 104667. https://doi.org/10.1016/j.pestbp.2020.104667

Gaire, S., Zheng, W., Scharf, M. E., & Gondhalekar, A. D. (2021). Plant essential oil constituents enhance deltamethrin toxicity in a resistant population of bed bugs (Cimex lectularius L.) by inhibiting cytochrome P450 enzymes. Pesticide Biochemistry and Physiology, 175, 104829. https://doi.org/10.1016/j.pestbp.2021.104829

Germano, M. D., & Picollo, M. I. (2018). Stage-dependent expression of deltamethrin toxicity and resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina. Journal of Medical Entomology, 55(4), 964–968. https://doi.org/10.1093/jme/tjy017

Giatropoulos, A., Papachristos, D. P., Kimbaris, A., Koliopoulos, G., Polissiou, M. G., Emmanouel, N., & Michaelakis, A. (2012). Evaluation of bioefficacy of three Citrus essential oils against the dengue vector Aedes albopictus (Diptera: Culicidae) in correlation to their components enantiomeric distribution. Parasitology Research, 111(6), 2253–2263. https://doi.org/10.1007/s00436-012-3074-8

Govindarajan, M., Rajeswary, M., Hoti, S. L., Bhattacharyya, A., & Benelli, G. (2016). Eugenol, α-pinene and β-caryophyllene from Plectranthus barbatus essential oil as eco-friendly larvicides against malaria, dengue and Japanese encephalitis mosquito vectors. Parasitology Research, 115(2), 807–815. https://doi.org/10.1007/s00436-015-4809-0

Liu, Z., Li, Q. X., & Song, B. (2022). Pesticidal activity and mode of action of monoterpenes. Journal of Agricultural and Food Chemistry, 70(15), 4556– 4571. https://doi.org/10.1021/acs.jafc.2c00635

Moretti, A. N., Zerba, E. N., & Alzogaray, R. A. (2013). Behavioral and toxicological responses of Rhodnius prolixus and Triatoma infestans (Hemiptera: Reduviidae) to 10 monoterpene alcohols. Journal of Medical Entomology, 50(5), 1046–1054. https://doi.org/10.1603/ME12248

Mougabure-Cueto G., & Picollo, M. I. (2021). Insecticide resistance in triatomines. In: A. Guarneri, & M. Lorenzo (Ed.), Triatominae — The biology of Chagas disease vectors (pp. 537–555). Entomology in Focus. Springer International Publishing. https://doi.org/10.1007/978-3-030-64548-9_19

OMS (Organización Mundial de la Salud). (1994). Protocolo de evaluación de efecto insecticida sobre triatominos. Acta Toxicológica Argentina, 2, 29–32.

OPS/OMS (Organización Panamericana de la Salud). (s. f.) Enfermedad de chagas. https://www.paho.org/es/temas/enfermedad-chagas.

Pavela, R. (2016). History, presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects: a review. Plant Protection. Science, 52, 229–241. https://doi.org/10.17221/31/2016-PPS

Picollo, M. I., Vassena, C., Santo, P., Barrios, S., Zaidemberg, M., & Zerba, E. (2005) High resistance to pyrethroid insecticides associated with ineffective field treatments in Triatoma infestans (Hemiptera: Reduviidae) from Northern Argentina. Journal of Medical Entomology, 42(4), 637–642. https://doi.org/10.1093/jmedent/42.4.637

Remón, C., Lobbia, P., Zerba, E., Mougabure-Cueto, G. A. (2017). Methodology based on insecticide impregnated filter paper for monitoring resistance to deltamethrin in Triatoma infestans field populations. Medical and Veterinary Entomology, 31(4), 414–426. https://doi.org/10.1111/mve.12252

Reynoso, M. N. N., Lucia, A., Zerba, E. N., & Alzogaray, R. A. (2020). The octopamine receptor is a possible target for eugenol-induced hyperactivity in the blood-sucking bug Triatoma infestans (Hemiptera: Reduviidae). Journal of Medical Entomology, 57(2), 627–630. https://doi.org/10.1093/jme/tjz183

Robertson, J. L., Russell, R. M., Preisler, H. K., & Savin, N. E. (2007). Bioassays with arthropods (2nd ed.). CRC Press. https://doi.org/10.1201/9781420004045

Sfara, V., Zerba, E. M., & Alzogaray, R. A. (2006) Toxicity of pyrethroids and repellency of diethyltoluamide in two deltamethrin-resitant colonies of Triatoma infestans Klug, 1834 (Hemiptera: Reduviidae). Memórias do Instituto Oswaldo Cruz, 101(1), 89-94. https://doi.org/10.1590/S0074-02762006000100017

Sfara, V., Zerba, E. N., & Alzogaray, R. A. (2009). Fumigant insecticidal activity and repellent effect of five essential oils and seven monoterpenes on first-instar nymphs of Rhodnius prolixus. Journal of Medical Entomology, 46(3), 511–515. https://doi.org/10.1603/033.046.0315

Tapondjou, A. L., Adler, C., Fontem, D. A., Bouda, H., & Reichmut, C. (2005) Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucaliptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. Journal of Stored Products Research, 41(1), 91–102. https://doi.org/10.1016/j.jspr.2004.01.004