Skip to main navigation menu Skip to main content Skip to site footer

Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

Abstract

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

Keywords

Native species, tissue culture, rooting, microcutting, explant, growth regulators

PDF

Author Biography

Paulo Mauricio Centenaro Bueno

Agronomist, PhD in Plant Sciences.

Professor at IFPR, teaching plant phisiology, plant breeding, fruitculture and anual crops.

Luiz Antonio Biasi

Departamento de Fitotecnia e Fitossanitarismo

Setor de Ciências Agrárias

Mauro Brasil Dias Tofanelli

Departamento de Fitotecnia e Fitossanitarismo

Setor de Ciências Agrárias


References

  1. Augusto, C.S.S., L.A. Biasi, and C.A. Telles. 2006. Enraizamento e aclimatização de plantas micropropagadas de amoreira-preta cv. Brazos. Rev. Bras. Frutic. 28(3), 473-476. Doi: 10.1590/S0100-29452006000300029
  2. Campagnolo, M.A. and R. Pio. 2012. Enraizamento de estacas caulinares e radiculares de cultivares de amoreira-preta coletadas em diferentes épocas, armazenadas a frio e tratadas com IBA. Ciênc. Rural 42, 232-237. Doi: 10.1590/S0103-84782012000200008
  3. Clapa, D., A. Fira and N. Joshee. 2013. An efficient ex vitro rooting and acclimatization method for horticultural plants using float hydroculture. HortScience 48(9), 1159-1167.
  4. Cordeiro, J., C.V. Roderjan, and W.A. Rodrigues. 2011. Plantas lenhosas da floresta ombrófila mista do parque municipal das Araucárias – Guarapuava (PR). Ambiência 7, 441-460. Doi: 10.5777/ambiencia.2011.03.03
  5. Debnath, S.C. 2003. Micropropagation of small fruits. pp. 465-506. In: Jain, S.M. and K. Ishii (eds.). Micropropagation of woody trees and fruits. Kluwer Academic Publishers, Dordrecht, The Netherlands. Doi: 10.1007/978-94-010-0125-0_15
  6. Debnath, S.C. 2004. Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation. Plant Growth Reg. 43, 179-186. Doi: 10.1023/B:GROW.0000040110.53216.6a
  7. Del Castillo, A.R. and A.A. Zerda. 1990. Estudios preliminaries para la propagación clonal in vitro de mora (Rubus glaucus L.). Agron. Colomb. 7(1-2), 17-25.
  8. Deng, R. and D.J. Donnelly. 1993. In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium. Can. J. Plant Sci. 73(4), 1105-1113. Doi: 10.4141/cjps93-149
  9. Erig, A.C., A. De Rossi, and G.R.L. Fortes. 2002. 6-benzilaminopurina e ácido indolbutírico na multiplicação in vitro da amoreira-preta (Rubus idaeus), cv. Tupy. Ciênc. Rural 32(5), 765-770. Doi: 10.1590/S0103-84782002000500005
  10. Jin, W., Y. Gu, and S.Z. Zhen. 1992. In vitro propagation of Rubus species. Sci. Hortic. 49(3-4), 335-340. Doi. 10.1016/0304-4238(92)90169-D
  11. Lazic, T. and D. Ruzic. 2007. Organogenesis in vitro from the leaf of blackberry cv. Cacanska Bestrna. Genetika 39, 69-78. Doi: 10.2298/GENSR0701069L
  12. Leitzke, L.N., C.R. Damiani, and M.W. Schuch. 2009. Meio de cultura, concentração de AIB e tempo de cultivo no enraizamento in vitro de amoreira-preta e framboeseira. Rev. Bras. Frutic. 31(2), 582-587. Doi: 10.1590/S0100-29452009000200037
  13. Leitzke, L.N., C.R. Damiani, and M.W. Schuch. 2010. Influência do meio de cultura, tipo e concentração de citocininas na multiplicação in vitro de amoreira-preta e framboeseira. Ciênc Agrotec. 34(2), 352-360. Doi: 10.1590/S1413-70542010000200012
  14. Maia, A.J. and R.V. Botelho. 2008. Reguladores vegetais no enraizamento de estacas lenhosas da amoreira-preta cv. Xavante. Semina: Ciênc. Agrár. 29, 323-330. Doi: 10.5433/1679-0359.2008v29n2p323
  15. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 15, 473-479. Doi: 10.1111/j.1399-3054.1962.tb08052.x
  16. Oliveira, A.F., N.N.J. Chalfun, A.A. Alvarenga, J. Vieira Neto, R. Pio, and D.L. Oliveira. 2009. Estaquia de oliveira em diferentes épocas, substratos e doses de AIB diluído em NaOH e álcool. Ciênc. Agrotec. 33(1), 79-85. Doi: 10.1590/S1413-70542009000100011
  17. Oliveira, R.P. and A.F.P. Nino. 2009. Potencial de multiplicação in vitro de cultivares de framboeseira. Rev. Bras. Frutic. 31(1), 280-284. Doi: 10.1590/S0100-29452009000100040
  18. Oliveira, R.P., A.F.P. Nino, and L.V. Ferreira. 2008. Potencial de multiplicação in vitro de cultivares de amoreira-preta. Rev. Bras. Frutic. 30(3), 585-589. Doi: 10.1590/S0100-29452008000300004
  19. Pasa, M.S., G.L. Carvalho, M.W. Schuch, J.D. Schmitz, M.M. Torchelsen, G.K. Nickel, L.R. Sommer, T.S. Lima, and S.S. Camargo. 2012. Qualidade de luz e fitorreguladores na multiplicação e enraizamento in vitro da amoreira-preta ‘Xavante’. Ciênc. Rural 42(8), 1392-1396. Doi: 10.1590/S0103-84782012000800010
  20. Pelizza, T.R., J. Muniz, P. Camargo, A.A. Kretzschmar, and L. Rufato. 2013. Enraizamento ex vitro e aclimatização de plântulas micropropagadas de amoreira-preta 'Xavante'. Rev. Bras. Frutic. 35(1), 329-332. Doi: 10.1590/S0100-29452013000100039
  21. Poothong, S. and B.M. Reed. 2015. Increased CaCl2, MgSO4, and KH2PO4 improve the growth of micropropagated red raspberries. In Vitro Cell Dev. Biol-Plant 51, 648-658. Doi: 10.1007/s11627-015-9720-y
  22. Sigarroa-Rieche, A.K. and C.L. García-Delgado. 2011. Establecimiento y multiplicación in vitro de mora de castilla (Rubus glaucus Benth) variedade sin espinas, mediante ápices meristemáticos. Acta Agron. 60(4), 347-354.
  23. Taiz, L. and E. Zeiger. 2013. Fisiologia vegetal. 5a ed. Artmed, Porto Alegre, Brazil.
  24. Villa, F., M. Pasqual, F.A. Assis, L.A.S. Pio, and G.A. Assis. 2008. Crescimento in vitro de amoreira-preta: efeito de reguladores de crescimento e da cultivar. Ciênc. Agrotec. 32(6), 1754-1759. Doi: 10.1590/S1413-70542008000600012
  25. Welander, M. 1985. In vitro culture of raspberry (Rubus idaeus) for mass propagation. J. Hortic. Sci. 60(4), 493-499. Doi: 10.1080/14620316.1985.11515656
  26. Wu, J., S.A. Miller, H.K. Hall, and P.A. Mooney. 2009. Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus. Plant Cell Tiss. Org. 99, 17-25. Doi: 10.1007/s11240-009-9571-5

Downloads

Download data is not yet available.

Most read articles by the same author(s)

Similar Articles

You may also start an advanced similarity search for this article.