Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)
DOI:
https://doi.org/10.17584/rcch.2018v12i2.7226Keywords:
Native species, tissue culture, rooting, microcutting, explant, growth regulatorsAbstract
This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.
Downloads
References
Augusto, C.S.S., L.A. Biasi, and C.A. Telles. 2006. Enraizamento e aclimatização de plantas micropropagadas de amoreira-preta cv. Brazos. Rev. Bras. Frutic. 28(3), 473-476. Doi: 10.1590/S0100-29452006000300029
Campagnolo, M.A. and R. Pio. 2012. Enraizamento de estacas caulinares e radiculares de cultivares de amoreira-preta coletadas em diferentes épocas, armazenadas a frio e tratadas com IBA. Ciênc. Rural 42, 232-237. Doi: 10.1590/S0103-84782012000200008
Clapa, D., A. Fira and N. Joshee. 2013. An efficient ex vitro rooting and acclimatization method for horticultural plants using float hydroculture. HortScience 48(9), 1159-1167.
Cordeiro, J., C.V. Roderjan, and W.A. Rodrigues. 2011. Plantas lenhosas da floresta ombrófila mista do parque municipal das Araucárias – Guarapuava (PR). Ambiência 7, 441-460. Doi: 10.5777/ambiencia.2011.03.03
Debnath, S.C. 2003. Micropropagation of small fruits. pp. 465-506. In: Jain, S.M. and K. Ishii (eds.). Micropropagation of woody trees and fruits. Kluwer Academic Publishers, Dordrecht, The Netherlands. Doi: 10.1007/978-94-010-0125-0_15
Debnath, S.C. 2004. Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation. Plant Growth Reg. 43, 179-186. Doi: 10.1023/B:GROW.0000040110.53216.6a
Del Castillo, A.R. and A.A. Zerda. 1990. Estudios preliminaries para la propagación clonal in vitro de mora (Rubus glaucus L.). Agron. Colomb. 7(1-2), 17-25.
Deng, R. and D.J. Donnelly. 1993. In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium. Can. J. Plant Sci. 73(4), 1105-1113. Doi: 10.4141/cjps93-149
Erig, A.C., A. De Rossi, and G.R.L. Fortes. 2002. 6-benzilaminopurina e ácido indolbutírico na multiplicação in vitro da amoreira-preta (Rubus idaeus), cv. Tupy. Ciênc. Rural 32(5), 765-770. Doi: 10.1590/S0103-84782002000500005
Jin, W., Y. Gu, and S.Z. Zhen. 1992. In vitro propagation of Rubus species. Sci. Hortic. 49(3-4), 335-340. Doi. 10.1016/0304-4238(92)90169-D
Lazic, T. and D. Ruzic. 2007. Organogenesis in vitro from the leaf of blackberry cv. Cacanska Bestrna. Genetika 39, 69-78. Doi: 10.2298/GENSR0701069L
Leitzke, L.N., C.R. Damiani, and M.W. Schuch. 2009. Meio de cultura, concentração de AIB e tempo de cultivo no enraizamento in vitro de amoreira-preta e framboeseira. Rev. Bras. Frutic. 31(2), 582-587. Doi: 10.1590/S0100-29452009000200037
Leitzke, L.N., C.R. Damiani, and M.W. Schuch. 2010. Influência do meio de cultura, tipo e concentração de citocininas na multiplicação in vitro de amoreira-preta e framboeseira. Ciênc Agrotec. 34(2), 352-360. Doi: 10.1590/S1413-70542010000200012
Maia, A.J. and R.V. Botelho. 2008. Reguladores vegetais no enraizamento de estacas lenhosas da amoreira-preta cv. Xavante. Semina: Ciênc. Agrár. 29, 323-330. Doi: 10.5433/1679-0359.2008v29n2p323
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 15, 473-479. Doi: 10.1111/j.1399-3054.1962.tb08052.x
Oliveira, A.F., N.N.J. Chalfun, A.A. Alvarenga, J. Vieira Neto, R. Pio, and D.L. Oliveira. 2009. Estaquia de oliveira em diferentes épocas, substratos e doses de AIB diluído em NaOH e álcool. Ciênc. Agrotec. 33(1), 79-85. Doi: 10.1590/S1413-70542009000100011
Oliveira, R.P. and A.F.P. Nino. 2009. Potencial de multiplicação in vitro de cultivares de framboeseira. Rev. Bras. Frutic. 31(1), 280-284. Doi: 10.1590/S0100-29452009000100040
Oliveira, R.P., A.F.P. Nino, and L.V. Ferreira. 2008. Potencial de multiplicação in vitro de cultivares de amoreira-preta. Rev. Bras. Frutic. 30(3), 585-589. Doi: 10.1590/S0100-29452008000300004
Pasa, M.S., G.L. Carvalho, M.W. Schuch, J.D. Schmitz, M.M. Torchelsen, G.K. Nickel, L.R. Sommer, T.S. Lima, and S.S. Camargo. 2012. Qualidade de luz e fitorreguladores na multiplicação e enraizamento in vitro da amoreira-preta ‘Xavante’. Ciênc. Rural 42(8), 1392-1396. Doi: 10.1590/S0103-84782012000800010
Pelizza, T.R., J. Muniz, P. Camargo, A.A. Kretzschmar, and L. Rufato. 2013. Enraizamento ex vitro e aclimatização de plântulas micropropagadas de amoreira-preta 'Xavante'. Rev. Bras. Frutic. 35(1), 329-332. Doi: 10.1590/S0100-29452013000100039
Poothong, S. and B.M. Reed. 2015. Increased CaCl2, MgSO4, and KH2PO4 improve the growth of micropropagated red raspberries. In Vitro Cell Dev. Biol-Plant 51, 648-658. Doi: 10.1007/s11627-015-9720-y
Sigarroa-Rieche, A.K. and C.L. García-Delgado. 2011. Establecimiento y multiplicación in vitro de mora de castilla (Rubus glaucus Benth) variedade sin espinas, mediante ápices meristemáticos. Acta Agron. 60(4), 347-354.
Taiz, L. and E. Zeiger. 2013. Fisiologia vegetal. 5a ed. Artmed, Porto Alegre, Brazil.
Villa, F., M. Pasqual, F.A. Assis, L.A.S. Pio, and G.A. Assis. 2008. Crescimento in vitro de amoreira-preta: efeito de reguladores de crescimento e da cultivar. Ciênc. Agrotec. 32(6), 1754-1759. Doi: 10.1590/S1413-70542008000600012
Welander, M. 1985. In vitro culture of raspberry (Rubus idaeus) for mass propagation. J. Hortic. Sci. 60(4), 493-499. Doi: 10.1080/14620316.1985.11515656
Wu, J., S.A. Miller, H.K. Hall, and P.A. Mooney. 2009. Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus. Plant Cell Tiss. Org. 99, 17-25. Doi: 10.1007/s11240-009-9571-5
Downloads
Published
How to Cite
Issue
Section
License
The copyright of the articles and illustrations are the property of the Revista Colombiana de Ciencias Hortícolas. The editors authorize the use of the contents under the Creative Commons license Attribution-Noncommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). The correct citation of the content must explicitly register the name of the journal, name (s) of the author (s), year, title of the article, volume, number, page of the article and DOI. Written permission is required from publishers to publish more than a short summary of the text or figures.
