Estimation and correlation of chlorophyll and nitrogen contents in Psidium guajava L. with destructive and non-destructive methods
- Luisa-Liliana Güiza-Castillo
, - Elberth-Hernando Pinzón-Sandoval,
- Pablo-Antonio Serrano-Cely,
- German-Eduardo Cely-Reyes,
- Pablo-Cesar Serrano-Agudelo
Abstract
Estimating photosynthetic pigments, such as chlorophylls, as well as the foliar nitrogen content is very important for the evaluation of the nutritional and physiological status of a plant. However, producers cannot easily carry out these estimations because they involve analyses in laboratories that are costly and time-consuming. The SPAD-502 total chlorophyll index meter is efficient at rapidly estimating total chlorophyll contents in situ, along with its relationship with the total nitrogen concentration in tissues. The objective of this research was to validate a non-destructive in situ method (SPAD) for use in place of destructive estimation methods for chlorophylls a, b and total, as well as the foliar nitrogen concentration in guava plants (Psidium guajaba L.), cv. Regional Roja, in three phenological stages under the conditions of the municipality of Vélez-Santander (Colombia). The data obtained with the SPAD-502 and the content of chlorophylls a, b and total were adjusted to a second-degree polynomial with correlation coefficients (r) greater than 0.91 for the three phenological stages, while the concentration of foliar nitrogen was adjusted to a linear type model with determination coefficients (R2) higher than 0.90, indicating a highly linear correlation between the non-destructive method and the destructive methods used in the present study.
Keywords
SPAD, Photosynthetic pigments, Mineral nutrition, Portable meter, Nutritional status
References
Amarante, C.V.T., O.Z. Zanardi, A. Miqueloto, C.A. Steffens, J. Erhart, and J.A.D. Almeida. 2009. Quantificação da área e do teor de clorofilas em folhas de plantas jovens de videira “Cabernet sauvignon” mediante métodos não destrutivos. Rev. Bras. Frutic. 31(3), 680-686. Doi: 10.1590/S0100-29452009000300009
Azia, F. and K.A. Stewart. 2001. Relationships between extractable chlorophyll and SPAD values in muskmelon leaves. J. Plant Nutr. 24(6), 961-966. Doi: 10.1081/PLN-100103784
Callejas, R., E. Kania, A. Contreras, C. Peppi, and L. Morales. 2014. Evaluación de un método no destructivo para estimar las concentraciones de clorofila en hojas de variedades de uva de mesa. Idesia (Arica), 31(4), 19-26. Doi: 10.4067/s0718-34292013000400003
Castañeda, C.S., P.J. Almanza-Merchán, E.H. Pinzón-Sandoval, G.E. Cely-Reyes, and P.A. Serrano-Cely. 2018. Estimación de la concentración de clorofila mediante métodos no destructivos en vid (Vitis vinifera L.) cv. Riesling Becker. Rev. Colomb. Cienc. Hortic. 12(2), 329-337. Doi: 10.17584/rcch.2018v12i2.7566
Castillo, Á.R. and G.A. Ligarreto. 2016. Relación entre nitrógeno foliar y el contenido de clorofila, en maíz asociado con pastos en el Piedemonte Llanero colombiano. Corpoica Cienc. Tecnol. Agropecu. 11(2), 122. Doi: 10.21930/rcta.vol11_num2_art:202
Donnelly, A., R. Rehberg C. Yu, G. Meyer, and E.B. Young. 2020. Leaf chlorophyll estimates of temperate deciduous shrubs during autumn senescence using a SPAD-502 meter and calibration with extracted chlorophyll. Ann. For. Sci. 77(2). Doi: 10.1007/s13595-020-00940-6
Fenech-Larios, L., E. Troyo-Diéguez, M. Trasviña-Castro, F. Ruiz-Espinoza, A. Beltrán-Morales, B. Murillo-Amador, J. García-Hernández, and S. Zamora-Salgado. 2009. Relación entre un método no destructivo y uno de extracción destructivo, para medir el contenido de clorofila en hojas de plántula de albahaca (Ocimum basilicum L). Univer. Cienc. 25(1), 99-102.
Hurtado, E., F. Gonzalez-Vallejos, C. Röper, E. Bastías, and P. Mazuela. 2017. Propuesta para la determinación del contenido de clorofila en hojas de tomate. Idesia 35(4), 129-130. Doi: 10.4067/S0718-34292017000400129
Jiang, C., M. Johkan, M. Hohjo, S. Tsukagoshi, and T. Maturo. 2017. A correlation analysis on chlorophyll content and SPAD value in tomato leaves. HortResearch (71), 37-42. Doi: 10.20776/S18808824-71-P37
Jifon, J.L., J.P. Syvertsen, and E. Whaley. 2005. Growth environment and leaf anatomy affect nondestructive estimates of chlorophyll and nitrogen in Citrus sp. leaves. J. Am. Soc. Hort. Sci. 130(2), 152-158. Doi: 10.21273/jashs.130.2.152
Lichtenthaler, H.K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol. 148(C), 350-382. Doi: 10.1016/0076-6879(87)48036-1
Malavolta, E., G.C. Vitti, and S.A. Oliveira. 1997. Avaliação do estado nutricional das. 2nd ed. Potafos, Piracicaba, Brazil.
Marschner, P. 2012. Mineral nutrition of higher plants. 3th ed. Elsevier, New York, NY.
Mendoza-Tafolla, R.O., P. Juarez-Lopez, R.E. Ontiveros-Capurata, M. Sandoval-Villa, I. Alia-Tejacal, and G. Alejo-Santiago. 2019. Estimating nitrogen and chlorophyll status of romaine lettuce using SPAD and at LEAF readings. Not. Bot. Horti. Agrobo. 47(3), 751-756. Doi: 10.15835/nbha47311525
Neves Caires, O.S., J.G.D. Carvalho, F.A.D. Martins, T.R.P.D. Pádua, and P.J.D. Pinho. 2005. Uso do SPAD-502 na avaliação dos teores foliares de clorofila, nitrogênio, enxofre, ferro e manganês do algodoeiro herbáceo. Pesq. Agropec. Bras. 40(5), 517-521. Doi: 10.1590/S0100-204X2005000500014
Ramírez Builes, V.H., M. Moreno Berrocal, and J.C. López Ruiz. 2012. Evaluación temprana de la deficiencia del nitrógeno en café y aplicaciones. Avan. Téc. Cenicafé (11), 1-7.
Rodríguez, M., G. Alcántar, A. Aguilar, J. Etchevers, and J. Santizó. 1998. Estimation of nitrogen and chlorophyll status of tomato with a portable chlorophyll meter. Terra 16(2), 135-141.
Salazar, D.M., P. Melgarejo, R. Martínez, J.J. Martínez, F. Hernández, and M. Burguera. 2006. Phenological stages of the guava tree (Psidium guajava L.). Sci. Hortic. 108(2), 157-161. Doi: 10.1016/j.scienta.2006.01.022
Solarte, M., L. Moreno, and L. Melgarejo. 2010. Fotosíntesis y pigmentos vegetales. pp. 107-122. In: Melgarejo, L. (ed.). Experimentos en fisiología y bioquímica vegetal. Departamento de Biología, Universidad Nacional de Colombia, Bogota.
Taiz, L. and E. Zeiger. 2010. Plant physiology. 3rd ed. Sinauer Associates, Sunderland, MA.
Torres Netto, A., E. Campostrini, J.G.D. Oliveira, and R.E. Bressan-Smith. 2005. Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves. Sci. Hortic. 104(2), 199-209. Doi: 10.1016/j.scienta.2004.08.013
Uddling, J., J. Gelang-Alfredsson, K. Piikki, and H. Pleijel. 2007. Evaluating the relationship between leaf chlorophyll concentration and SPAD-502 chlorophyll meter readings. Photosynth. Res. 91(1), 37-46. Doi: 10.1007/s11120-006-9077-5
Yue, X., Y. Hu, H. Zhang, and U. Schmidhalter. 2020. Evaluation of both SPAD reading and SPAD index on estimating the plant nitrogen status of winter wheat. Int. J. Plant Prod. 14(1), 67-75. Doi: 10.1007/s42106-019-00068-2
Zotarelli, L., E.G. Cardoso, J.L. Piccinin, S. Urquiaga, R.M. Boddey, E. Torres, and B.J.R. Alves. 2003. Calibração do medidor de clorofila Minolta SPAD-502 para avaliação do conteúdo de nitrogênio do milho. Pesq. Agropec. Bras. 38(9), 1117-1122. Doi: 10.1590/S0100-204X2003000900014