Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.))




Flower stigma, Peroxidase, Emasculation, Anthesis, Pollination


Classic plant breeding, based on the selection of superior individuals and directed crosses, led to the need-to-know aspects of the floral biology of cowpea beans. The research was carried out at the Universidad de Córdoba, Colombia, through two experiments: in the first, stigmatic receptivity was evaluated as response time to hydrogen peroxide, under a randomized complete block design, with a 3×4 factorial arrangement (three genotypes: Caupicor 50, Missouri and BRS Milenium, and at four hours of the day: 7:00 and 9:00 AM; 3:00 and 5:00 PM) and three replications. In the second, the percentage of viable crosses was evaluated, under a randomized complete block design, with a 2×2 factorial arrangement (two crosses: Missouri × IT86 and Missouri × BRS Milenium, and two methods: 1 (morning) and 2 (afternoon), and four replications. The greatest stigmatic receptivity was recorded in the BRS Milenium and Missouri genotypes with a time of 3.28±0.07 and 2.01±0.12 min at 7:00 and 9:00 AM, while Caupicor 50, time of 1.80±0.09 min at 3:00 PM. The artificial hybridizations carried out in the morning (method 1) registered the highest viable crosses, 78.6% in Missouri × BRS Milenium and 57.1% in Missouri × IT86. Therefore, artificial hybridizations should be done in the morning due to a more favorable environment for pollen grain germination, given the greater stigmatic receptivity.


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Hybridization in cowpea beans. Photo: H. Araméndiz-Tatis



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Araméndiz-Tatis, H, Cardona-Ayala, C, Espitia-Camacho, M, & Hernández-Murillo, J R. (2022). Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.)). Revista Colombiana de Ciencias Hortícolas, 16(2), e13820.



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