Morphometry, viability and germination of seeds of Benincasa hispida [(Thunb.) Cogn.]




Winter gourd, Biometric characteristics, Tetrazolium test, Physiological quality


The objective of the study was to evaluate the morphometry, viability and germination of B. hispida seeds, at the Universidad de Córdoba, Colombia. Eight biometric characteristics were measured in a sample of seeds taken at random from a balanced compound originating from 50 fruits. The external and internal morphological description was made on a sample of 10 seeds. Seed viability was estimated by the tetrazolium test, using the combination of two staining times (2 and 3 hours) and three concentrations of (0.5, 1.0 and 1.5%), under a design completely randomized, with six treatments and four repetitions of 50 seeds. The germination test (PRUGER) was performed in a mesh house with four repetitions of 50 seeds to estimate eight physiological parameters of germination. The morpho-metric characteristics of thickness, length and width of seeds showed reduced phenotypic variation, compared with the weight, density and volume of a seed, with variation coefficients greater than 11.7%. The seed, ovoid and flattened, presents an embryo with its cotyledons wrapped in a protective membrane. The viability, determined with tetrazolium, was 89.5-96.5%, while the PRUGER was 100%, with no statistical differences between them (P≥0.05). The combination of 0.5% tetrazolium with 2 hours is sufficient to determine the viability of the seed. Germination elapsed from 6 to 19 days after sowing, with a germination speed index of 0.22, average daily germination of 5.26%, peak value of 5.33 and germination value of 28.


Download data is not yet available.


Agbagwa, I.O. and B.C. Ndukwu. 2004. The value of morpho-anatomical features in the systematics of Cucurbita L. (Cucurbitaceae) species in Nigeria. Afr. J. Biotechnol. 3(10), 541-546. Doi: 10.5897/AJB2004.000-2106

Cañadas-López, Á.G., D.Y. Rade-Loor, R.O. Quijije-Pinargote, I.A. Sotomayor, and A.M. Ormaza-Molina. 2018. Assessment of 112 tomato (Solanum lycopersicum L.) cultivars for industrial processing in Portoviejo, Ecuador. Acta Agron. 67(2), 347-354. Doi: 10.15446/acag.v67n2.62725

Chomicki, G., H. Schaefer, and S.S. Renner. 2020. Origin and domestication of Cucurbitaceae crops: insights from phylogenies, genomics and archaeology. New Phytol. 226(5), 1240-1255. Doi: 10.1111/nph.16015

Cruz, C.D. 2016. Programa Genes V.2014.6.1 - Aplicativo computacional em genética e estatística. In:; consulted: September, 2016

Czabator, F. 1962. Germination value: An index combining speed and completeness of pine seed germination. For. Sci. (USA) 8(4), 386-396.

Delgado, G., C. Rojas, A. Sencie, and L. Vásquez. 2014. Caracterización de frutos y semillas de algunas cucurbitáceas en el norte del Perú. Rev. Fitotec. Mex. 37(1), 7-20. Doi: 10.35196/rfm.2014.1.7

Dutta, P., S. Hazari, C. Karak, and S. Talukdar. 2018. Study on genetic variability of different tomato (Solanum lycopersicum) cultivars grown under open field condition. Int. J. Chem. Stud. 6(5), 1706-1709.

Ekeke, C., C.A. Ogazie, and I.O. Agbagwa. 2019. Anatomical and Phytochemical Studies on Benincasa hispida (Thunb.) Cogn. (Cucurbitaceae). Not. Sci. Biol. 11(1), 102-111. Doi: 10.15835/nsb11110394

Espitia, M., C. Cardona, and H. Araméndiz. 2017. Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers. Agron. Mesoam. 28(3), 605-617. Doi: 10.15517/ma.v28i3.26287

Fantazzini, T.B., S.D.V.F. da Rosa, G.R. Carvalho, G.R. Liska, M.L.M. Carvalho, S.V.B. Coelho, M.A. Cirillo, and F.A.V. Ribeiro. 2020. Correlation between historical data of the germination test and of the tetrazolium test in coffee seeds by GAMLSS. Seed Sci. Technol. 48(2), 179-188. Doi: 10.15258/sst.2020.48.2.05

Hartmann-Filho, C.P., A.L.D. Goneli, T.E. Masetto, E.A.S. Martins, and G.C. Oba. 2016. The effect of drying temperatures and storage of seeds on the growth of soybean seedlings. J. Seed Sci. 38(4), 287-295. Doi: 10.1590/2317-1545v38n4161866

Hidayat, T.R.S. and A. Ridhawati. 2020. The vigor and viability seed testing of three tobacco varieties on various seed germination media. Agrotech. J. 5(1), 1-9. Doi: 10.31327/atj.v5i1.1210

Lima, L.B., T.L. Pinto, and A.D. Novembre. 2010. Avaliação da viabilidade e do vigor de sementes de pepino pelo teste de tetrazolio. Rev. Bras. Sementes 32(1), 60-68. Doi: 10.1590/S0101-31222010000100007

Macedo-Sousa, D.M., R.D. Alcântara-Bruno, C.D. Gomes-Silva, S. Barros-Torres, and A. Pereira-Andrade. 2017. Viabilidade e vigor de sementes de Poincianella pyramidalis (Tul.) L. P. Queiroz pelo teste de tetrazólio. Rev. Cien. Agron. 48(2), 381-388. Doi: 10.5935/1806-6690.20170044

Maguire, D. 1962. Speed of germination-aid. Selection and evaluation for seedling emergence and vigour. Crop Sci. 2(1), 176-177. Doi: 10.2135/cropsci1962.0011183X000200020033x

Mandana, B., A.R. Russly, S.T. Farah, M.A. Noranizan, I.S. Zaidul, and G. Ali. 2012. Antioxidant activity of winter melon (Benincasa hispida) seeds using conventional Soxhlet extraction technique. Int. Food Res. J. 19(1), 229-234.

MAPAB, Ministério da Agricultura, Pecuária e Abastecimento do Brasil. 2009. Regras para análise de sementes. Ministério da Agricultura, Pecuária e Abastecimento do Brasil. In:; consulted: October, 2018.

Mercado, S.A.S. and E.A.B. Delgado. 2018. Viabilidad de semillas de Glycine max (l.) Utilizando la prueba de tetrazolio. Rev. Inv. Agra. Amb. 9(2), 4-12.

Niembro, A. 1988. Semillas de árboles y arbustos: Ontogenia y estructura. Editorial Limusa, Mexico, DC.

Pandey, A.K., D.R. Bhardwaj, R.K.Dubey, V. Singh, and S. Pandey. 2015. Botany, diversity, utilization and improvement of ash gourd (Benincasa Hispida THUNB. Ex Murray Cogn)—A review. Ann. Hort. 8(1), 1-15.

Pinto, T.L.F., J.M. Filho, V.A. Forti, C. Carvalho, and F.G. Gomes. 2009. Avaliação da viabilidade de sementes de pinhão manso pelos testes de tetrazólio e de raios X. Rev. Bras. Sementes 31(2), 195-201. Doi: 10.1590/S0101-31222009000200023

Pradhan, K., A. Nandi, S. Rout, and B. Tripathy. 2020. Ash gourd - an under exploited potential crop. Dogo Rangsang Res. J. 10(06), 142-151. Doi: 10.20546/ijcmas.2018.703.258

Rao, N.K., J. Hanson, M.E. Dulloo, K. Ghosh, D. Novell, and M. Larinde. 2007. Manual para el manejo de semillas en bancos de germoplasma. Manuales para Bancos de Germoplasma No. 8. Bioversity International, Roma.

Shafique, M., M. Ahsan, Z. Mehmood, M. Abdullah, A. Shakoor, and M. Ahmad. 2016. Genetic variability and interrelationship of various agronomic traits using correlation and path analysis in Chickpea (Cicer arietinum L.). Acad. J. Agric. Res. 4(2), 082-085.

Souza, C.R., O.C. Ohlson, and M. Panobianco. 2010. Avaliação da viabilidade de sementes de aveia branca pelo teste de tetrazólio. Rev. Bras. Sementes 32(1), 174-180. Doi: 10.1590/S0101-31222010000400020

Steiner, F., A.M. Zuffo, A. Busch, T.D.O. Sousa, and T. Zoz. 2019. Does seed size affect the germination rate and seedling growth of peanut under salinity and water stress? Pesq. Agropecu. Trop. 49(1), e54353. Doi: 10.1590/1983-40632019v4954353
B. hispida seeds germination. Photo: A. Villalba-Soto




How to Cite

Esptia-Camacho, M., Villalba-Soto, A., Aramédiz-Tatis, H., & Cardona-Ayala, C. (2021). Morphometry, viability and germination of seeds of Benincasa hispida [(Thunb.) Cogn.]. Revista Colombiana De Ciencias Hortícolas, 15(1), e11724.



Vegetable section