Skip to main navigation menu Skip to main content Skip to site footer

Effect of drying method and storage conditions on the essential oil yield and composition in Eugenia uniflora L. leaves

Supporting Agencies
CAPES – Brazilian Agency Foundation for Research

Eugenia uniflora (detail: leaves) Photo: R.R. Cipriano

Abstract

The essential oil produced by Eugenia uniflora leaves is intensively used by the cosmetic, pharmaceutic and perfumery industries mainly because its antioxidant effect and antimicrobial and antihelmintic ativities. Drying and storage of the aromatic plant leaves can affect the yield and quality of its essential oil. This study aimed to evaluate the effect of drying method and storage conditions on the essential oil yield and composition in Eugenia uniflora leaves. The essential oil were extracted from dried leaves at ambient temperature and at 45°C for six drying periods (0, 6, 24, 48, 72 and 96 hours-h). The essential oil yield was also analysed after 0, 60, 120, and 180 days of storage in three types of packaging. The oil was extracted by hydrodistillation and the composition was determined by gas chromatography-mass spectrometry. Results showed that drying the leaves at ambient temperature and 45°C for 48 and 24 h, respectively, increased the essential oil yield. At 45°C the monoterpene and sesquiterpene compounds increased, with curzerene+viridiflorene identified as the major compounds. Package types did not affect the essential oil yield but the increase of the storage period decreased it.

Keywords

Post-harvest, Aromatic plants, Sesquiterne compounds, Curzerene viridiflorene, Surinam cherry

PDF

References

Abdelmageed, A.H.A., Q.Z. Faridah, A. Nur Amalina, and M. Yaacob. 2011. The influence of organ and post-harvest drying period on yield and chemical composition of the essential oils of Etlingera elatior (Zingiberaceae). J. Med. Plants Res. 5(15), 3432-3439.

Adams, R.P. 2017. Identification of essential oil components by gas chromatography/ mass spectrometry. 4th ed. Allured Publishing Corporation, Carol Stream, IL.

Amaral, W., C. Deschamps, L.A. Biasi, H.R. Bizzo, M.P. Machado and L.E. Silva. 2018b. Yield and chemical composition of the essential oil of species of the Asteraceae family from Atlantic Forest, South of Brazil. J. Essent. Oil Res. 30(4), 278-284. Doi: https://doi.org/10.1080/10412905.2018.1434092

Amaral, W., C. Deschamps, L.E. Silva, H.R. Bizzo, M.A.S. Pinto, and L.A. Biasi. 2019. Essential oil yield and composition of native species of the Myrtaceae family from “Campos Gerais” of the Atlantic Forest in Parana State. Ciênc. Nat. 41, e45. Doi: https://doi.org/10.5902/2179460X40405

Amorim, A.C.L., C.K.F. Lima, A.M.C. Howell, A.L.P. Miranda, and C.M. Rezende. 2009. Antinociceptive and hypothermic evaluation of the leaf essential oil and isolated terpenoids from Eugenia uniflora L. (Brazilian Pitanga). Phytomedicine 16, 923-928. Doi: https://doi.org/10.1016/j.phymed.2009.03.009

ANVISA, Agência Nacional de Vigilância Sanitária. 2010. Farmacopéia brasileira. Vol. 2. 5a ed. Brasilia, DF. In: http://www.anvisa.gov.br/hotside/cd_farcopeia/index.htm; consulted: March, 2016.

Argyropoulos, D. and J. Müller. 2014. Changes of essential oil content and composition during convective drying of lemon balm (Melissa officinalis L.). Ind. Crops Prod. 52, 118-124. Doi: https://doi.org/10.1016/j.indcrop.2013.10.020

Costa, D.P., E.G. Alves-Filho, L.M.A. Silva, S.C. Santos, X.S. Passos, M.R.R. Silva, J.C. Seraphin, and P.H. Ferri. 2010. Influence of fruit biotypes on the chemical composition and antifungal activity of the essential oils of Eugenia uniflora leaves. J. Braz. Chem. Soc. 21(5), 851-858. Doi: https://doi.org/10.1590/S0103-50532010000500012

Costa, D.P., S.C. Santos, J.C. Seraphin, and P.H. Ferri. 2009. Seasonal variability of essential oils of Eugenia uniflora leaves. J. Braz. Chem. Soc. 20(7), 1287-1293. Doi: https://doi.org/10.1590/S0103-50532009000700013

Corrêa Jr., C., M.C. Scheffer, and L.C. Ming. 2006. Cultivo agroecológico de plantas medicinais, aromáticas e condimentares. Ministério do Desenvolvimento Agrário, Brasilia, DF. pp. 58-65.

Dušková, E., K. Dušek, P. Indrák, and K. Smékalová. 2016. Postharvest changes in essential oil content and quality of lavender flowers. Ind. Crops Prod. 79, 225-231. Doi: https://doi.org/10.1016/j.indcrop.2015.11.007

Fiuza, T.S., M.H. Rezende, S.M.T. Sabóia, M.T. Bara, L.M.F. Tresvenzol, and J.R. Paula. 2008. Caracterização farmacognóstica das folhas de Eugenia uniflora L. (Myrtaceae). Rev. Eletronica. Farm. 5(2), 21-31. Doi: https://doi.org/10.5216/ref.v5i2.5148

Gallucci, S., A. Placeres-Neto, C. Porto, D. Barnizan, I. Costa, K. Marques, P. Benevides, and R. Figueiredo. 2010. Essential oil of Eugenia uniflora L.: an industrial perfumery approach. J. Essent. Oil Res. 22, 176-179. Doi: https://doi.org/10.1080/10412905.2010.9700296

Hannaa, M., Y.I. Sallam, A.S. El-Leithy, and S.E. Aly. 2012. Lemongrass (Cymbopogon citratus) essential oil as affected by drying methods. Ann. Agric. Sci. 57(2), 113-116. Doi: https://doi.org/10.1016/j.aoas.2012.08.004

Harbourne, N., E. Marete, J.C. Jacquier, and D. O’Riordan. 2009. Effect of drying methods on the phenolic constituents of meadowsweet (Filipendula ulmaria) and willow (Salix alba). J. Food Sci. Technol. 42, 1468-1473. Doi: https://doi.org/10.1016/j.lwt.2009.05.005

Lisboa, C.F., E.C. Melo, and S.M.L. Donzeles. 2018. Influence of storage conditions on quality attributes of medicinal plants. Biomed. J. Sci. Tech. Res. 4(4), 4093-4095. Doi: https://doi.org/10.26717/BJSTR.2018.04.001097

Lorenzi, H. and F.J.A. Matos. 2008. Plantas medicinais no Brasil: nativas e exóticas. 2nd ed. Plantarum, Nova Odessa, SP.

Melo, R.M., V.F.S. Corrêa, A.C.L. Amorim, A.L.P. Miranda, and C.M. Rezende. 2007. Identification of impact aroma compounds in Eugenia uniflora L. (Brazilian Pitanga) leaf oil. J. Braz. Chem. Soc. 18(1), 179-183. Doi: https://doi.org/10.1590/S0103-50532007000100020

Ogunwande, I.A., N.O. Olawore, O. Ekundayo, T.M. Walker, J.M. Schmidt, and W.N. Setzer. 2015. Studies on the essential oils composition, antibacterial and cytotoxicity of Eugenia uniflora L. Int. J. Aromather. 15, 147-152. Doi: https://doi.org/10.1016/j.ijat.2005.07.004

Pirbalouti, A.G., M. Oraine, M. Pouriamehr, and S.E. Babadi. 2013. Effects of drying methods on qualitative and quantitative properties of the essential oil of Bakhtiari savory (Satureja bachtiarica Bunge.). Ind. Crops Prod. 46, 324-327. Doi: https://doi.org/10.1016/j.indcrop.2013.02.014

Pripdeevech, P. and E. Chukeatirote. 2010. Chemical compositions, antifungal and antioxidant activities of essential oil and various extracts of Melodorum fruticosum L. flowers. Food Chem. Toxicol. 48, 2754-2758. Doi: https://doi.org/10.1016/j.fct.2010.07.002

Rahimmalek, M. and S.A.H. Goli. 2013. Evaluation of six drying treatments with respect to essential oil yield, composition and color characteristics of Thymys daenensis subsp. daenensis. Celak leaves. Ind. Crops Prod. 42, 613-619. Doi: https://doi.org/10.1016/j.indcrop.2012.06.012

Rocha, R.P., E.C. Elo, and L.L. Radünz. 2011. Influence of drying process on the quality of medicinal plants: a review. J. Med. Plants Res. 5(33), 7076-7084. Doi: https://doi.org/10.5897/JMPRX11.001

Rodrigues, K.A.F., L.V. Amorim, J.M.G. Oliveira, C.N. Dias, D.F.C. Moraes, E.H.A. Andrade, J.G.S. Maia, S.M.P. Carneiro, and F.A.A. Carvalho. 2013. Eugenia uniflora L. essential oil as a potential anti-Leishmania agent: effects on Leishmania amazonensis and possible mechanisms of action. Evid. Based Complement. Alternat. Med. 2013(Spe. Iss.), 279726. Doi: https://doi.org/10.1155/2013/279726

Santos, F.R., R. Braz-Filho, and R.N. Castro. 2015. Influência da idade das folhas de Eugenia uniflora L. na composição química do óleo essencial. Quím. Nova 38(6), 762-768. Doi: https://doi.org/10.5935/0100-4042.20150072

Santos, J.F.S., J.E. Rocha, C.F. Bezerra, M.K.N. Silva, Y.M.L.S. Matos, T.S. Freitas, A.T.L. Santos, R.P. Cruz, A.J.T. Machado, T.H.S. Rodrigues, E.S. Britos, D.L. Sales, W.O. Almeida, J.G.M. Costa, H.D.M. Coutinho, and M.F.B. Morais-Braga. 2018. Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp. Food Chem. 261, 233-239. Doi: https://doi.org/10.1016/j.foodchem.2018.04.015

Sározi, Sz., L. Sipos, Z. Kókai, Zs. Plihár, B. Szilvássy, and I. Novák. 2013. Effect of different drying techniques on the aroma profile of Thymus vulgaris analyzed by GC-MS and sensory profile methods. Ind. Crops Prod. 46, 210-216. Doi: https://doi.org/10.1016/j.indcrop.2013.01.028

Sellami, I.H., W.A. Wannes, I. Bettaied, S. Berrima, T. Chahed, B. Marzouk, and F. Lima. 2011. Qualitative and quantitative changes in the essential oil of Laurus nobilis L. leaves as affected by different drying methods. Food Chem. 126, 691-697. Doi: https://doi.org/10.1016/j.foodchem.2010.11.022

Shahhoseini, R., A. Estaji, N. Hosseini, M. Ghorbanpour, and R. Omidbaigi. 2013. The effect of different drying methods on the content and chemical composition of essential oil of lemon verbena (Lippia citriodora). J. Essent. Oil Bear. Pl. 16(4), 474-481. Doi: https://doi.org/10.1080/0972060X.2013.813270

Silva, V.P., C.C.F. Alves, M.L.D. Miranda, L.C. Bretanha, M.P. Balleste, G.A. Micke, E.V. Silveira, C.H.G. Martins, M.A.L. Ambrosio, T.S. Silva, D.C. Tavares, L.G. Magalhães, F.G. Silva, and M.B. Egea. 2018. Chemical composition and in vitro leischmanicidal, antibacterial and cytotoxic activities of essential oils of the Myrtaceae family occurring in the Cerrado biome. Ind. Crops Prod. 123, 638-645. Doi: https://doi.org/10.1016/j.indcrop.2018.07.033

Silva, F.A.Z. and C.A.V. Azevedo. 2016. The assistat software v. 7.7 and its use in the analysis of experimental data. Afr. J. Agric. Res. 11(39), 3733-3740. Doi: https://doi.org/10.5897/AJAR2016.11522

Stefanello, M.E.A., A.C.R.F. Pascoal, and M.J. Salvador. 2011. Essential oils from Neotropical Myrtaceae: chemical diversity and biological properties. Chem. Biodivers. 8, 73-94. Doi: https://doi.org/10.1002/cbdv.201000098

Van Den Dool, H. and P.D. Kratz. 1963. A generalisation of the retention index system including linear temperature programmed gasliquid chromatography. J. Chromatogr. 11, 463-471.

Venditti, A., A. Bianco, C. Frezza, and F. Conti. 2015. Essential oil composition, polar compounds, glandular trichomes and biological activity of Hyssopus officinalis subsp. aristatus (Godr.) Nyman from central Italy. Ind. Crops Prod. 77, 353-363. Doi: https://doi.org/10.1016/j.indcrop.2015.09.002

Verma, R.K., L. Rahman, S. Mishra, R.K. Verma, A. Chauhan, and A. Singh. 2011. Changes in essential oil content and composition of leaf and leaf powder of Rosmarinus officinalis cv. CIM-Hariyali during storage. Maejo Int. J. Sci. Technol. 5(2), 181-190.

Victoria, F.N., E.J. Lenardão, L. Savegnago, G. Perin, R.G. Jacob, D. Alves, W.P. Silva, A.S. Motta, and P.S. Nascente. 2012. Essential oil of the leaves of Eugenia uniflora L.: antioxidant and antimicrobial properties. Food Chem. Toxicol. 50, 2668-2674. Doi: https://doi.org/10.1016/j.fct.2012.05.002

Voung, Q.V., N. Zammit, B.R. Munro, S. Murchie, M.C. Bowyer, and C.J. Scarlett. 2015. Effect of drying conditions on physicochemical and antioxidant properties of Vitex agnus-castus leaves. J. Food Process. Pres. 39, 2562-2571. Doi: https://doi.org/10.1111/jfpp.12506

Zuzarte, M. and L. Salgueiro. 2015. Essential oils chemistry. pp. 19-61. In: Sousa, D.P. (ed.). Bioactive essential oils and cancer. Springer International Publishing, Cham, Switzerland. Doi: https://doi.org/10.1007/978-3-319-19144-7

Downloads

Download data is not yet available.

Most read articles by the same author(s)

Similar Articles

1 2 3 > >> 

You may also start an advanced similarity search for this article.