Skip to main navigation menu Skip to main content Skip to site footer

Effects of nitrogen fertilization on growth and yield of diterpene glycosides in stevia (Stevia rebaudiana Bertoni)

Supporting Agencies
CAPES, Universidade Federal do Paraná, Instituto Federal Catarinense

Matrices of Stevia rebaudiana.  Photo: R.J. Debarba

Abstract

The effects of different nitrogen doses on dry weight yield, stevioside and rebaudioside A contents, and yields were evaluated in stevia (Stevia rebaudiana Bertoni). This study was carried out at the Instituto Federal Catarinense, Rio do Sul (SC), Brazil, under decreasing photoperiods. A randomized complete block design with four blocks and five treatments (0, 65, 135, 200 and 270 kg ha-1 N) was used. Variables related to dry weight yield (total dry weight, leaf dry weight, stem dry weight, number of secondary branches and tertiary branches, and main stem length), growth rates (total leaf area, leaf area index, specific leaf area, leaf area ratio, leaf weight ratio), and stevioside and rebaudioside A contents and yields were evaluated. The doses of nitrogen fertilization did not affect the variables related to dry weight yield, stevioside and rebaudioside A contents and yields, or rebaudioside A:stevioside ratio in stevia genotype 8 (G8), grown under decreasing photoperiods.

Keywords

Steviol glycosides, Photoperiod, Urea, Growth rates

PDF

References

Aladakatti, Y.R., Y.B. Palled, M.B. Chetti, S.I. Halikattim, S.C. Alagundagi, P.L. Patil, V.C. Patil, and A.D. Janawade. 2012. Effect of nitrogen, phosphorus and potassium levels on growth and yield of stevia (Stevia rebaudiana Bertoni). Karnataka J. Agric. Sci. 25(1), 25-29.

Akbari, F., A. Arminian, D. Kahrizi, A. Fazeli, and M. Ghaheri. 2018. Effect of nitrogen sources on gene expression os Stevia rebaudiana (Bertoni) under in vitro conditions. Cell. Mol. Biol. 64(2), 11-16. Doi: 10.14715/cmb/2018.64.2.3

Benincasa, M.M.P. 2003. Análise de crescimento de plantas: noções básicas. FUNEP, Jaboticabal, Brazil.

Cantarella, H. 2007. Nitrogênio. pp. 375-470. In: Novais, R.F., V.H. Alvarez V., N.F. Barros, R.L.F. Fontes, R.B. Cantarutti, and J.C.L. Neves (eds.). Fertilidade do solo. Sociedade Brasileira de Ciência do Solo, Vicosa, Brazil.

Carvalho, M.A.M., M.M. Pinto, and R.C.L. Figueiredo-Ribeiro. 1998. Inulin production by Vernonia hebacea as influenced by mineral fertilization and time of harvest. Rev. Bras. Bot. 21, 275-280. Doi: 10.1590/S0100-84041998000300006

Carvalho, M.A.M. and L.B.P. Zaidan. 1995. Obtenção de plantas de Stevia rebaudiana através de estacas. Pesq. Agropec. Bras. 30(2), 201-206.

Castro, E.M., J.E.B.P. Pinto, A.A. Alvarenga, E.D.C.L. Júnior, S.K.V. Bertolucci, J.L.D.S. Filho, and C.V. Vieira. 2003. Crescimento e anatomia foliar de plantas jovens de Mikania glomerata Sprengel (Guaco) submetidas a diferentes fotoperíodos. Cienc. Agrotec. 27(6), 1293-1300. Doi: 10.1590/S1413-70542003000600013

Ceunen, S. and J.M.C. Geuns. 2013a. Spatio-temporal variation of the diterpene steviol in Stevia rebaudiana grown under different photoperiods. Phytochemistry 89, 32-38. Doi: 10.1016/j.phytochem.2013.01.007

Ceunen, S. and J.M.C. Geuns. 2013b. Glucose, sucrose, and steviol glycoside accumulation in Stevia rebaudiana grown under different photoperiods. Biol. Plant. 57, 390-394. Doi: 10.1007/s10535-012-0289-6

Ceunen, S. and J.M.C. Geuns. 2013c. Influence of photoperiodism on the spatiotemporal accumulation of steviol glycosides in Stevia rebaudiana (Bertoni). Plant Sci. 198, 72-82. Doi: 10.1016/j.plantsci.2012.10.003

Cunha, J.L.X.L., P.G.M.L. Naascimento, H.C. Mesquita, M.G.O. Silva, J.L.D. Dombroski, and I.N. Silva. 2010. Comparação de métodos de área foliar em Chrysobalanus icaco L. ACSA -Agropec. Cient. Semi-Árido 6(3), 22-27.

Duncan, R.R. and V.C. Baligar. 1990. Genetics, breeding, and physiological mechanisms of nutrient uptake and use efficiency: an overview. pp. 3-35. In: Duncan, V.C. and R.R. Baligar (eds.). Crops as enhances of nutrient use. Academic Press, New York, NY. Doi: 10.1016/B978-0-12-077125-7.50005-0

Embrapa. 2018. Sistema brasileiro de classificação de solos. 2nd ed. Rio de Janeiro, Brazil.

Espita, M., R. Montoya, and L. Atencio. 2009. Rendimiento de Stevia rebaudiana Bert. bajo tres arreglos poblacionales en el Sinú Medio. Rev. UDCA Actual. Divulg. Cient. 12(1), 151-161. Doi: 10.31910/rudca.v12.n1.2009.651

Francisco, F. 2015. Acúmulo de biomassa, produção de esteviosídeo e rebaudiosídeo A e tolerância à geada e ao acamamento de acessos de Stevia rebaudiana bert. MSc thesis. Universidade Federal do Paraná, Parana, Brazil.

Gobbo-Neto, L. and N.P. Lopes. 2007. Medicinal plants: factors of influence on the content of secondary metabolites. Quím. Nova 30(2), 374-381. Doi: 10.1590/S0100-40422007000200026

Hsieh, M.-H. and H.M. Goodman. 2005. The Arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis. Plant Physiol. 138(2), 641-653. Doi: 10.1104/pp.104.058735

Jarma, A., T. Rengifo, and H. Araméndez-Tatis. 2006. Fisiología de estevia (Stevia rebaudiana) en función de la radiación en el Caribe colombiano. II. Análisis de crecimiento. Agron. Colomb. 24(1), 38-47.

Kolb, N., J.L. Herrera, D.J. Ferreyra, and R.F. Uliana. 2001. Analysis of sweet diterpeno glycosides from Stevia rebaudiana: improved HPLC method. J. Agric. Food Chem. 49 (10), 4538-4541. Doi: 10.1021/jf010475p

Lima Filho, O.F., A.C.C. Valois, and Z.M. Lucas. 2004. Sistema de produção 5: Estévia. Embrapa Agropecuaria Oeste; Steviafarma Industrial, Dourados, Brazil.

Magalhães, A.C.N. 1986. Análise quantitativa do crescimento. pp. 331-350. In: Ferri, M.G. (ed.). Fisiologia vegetal. 2nd ed. rev. e atual. EPU/EDUSP, São Paulo, Brazil.

Mota, T.R., A.S. Dacome, and S.C. Costa. 2011. Seleção de clones de elite de Stevia rebaudiana (Bert.) Bertoni com ênfase no teor do rebaudiosídeo A e a atividade antioxidante. Anais eletrônicos. In: Proc. VII EPCC – Encontro Internacional de Produção Científica. CESUMAR, Maringá, Parana, Brazil.

Jarma Orozco, A., M. Espitia Camacho, and G. Fischer. 2010. Síntesis de esteviosidos en estévia (Stevia rebaudiana Bert.). Acta Biol. Colomb. 15(1), 289-294.

Pal, P.K., M. Mahajan, R. Prasad, V. Pathania, N. Singh, and P.S. Ahuja. 2015. Harvesting regimes to optimize yield and quality in annual and perennial Stevia rebaudiana under sub-temperate conditions. Ind. Crops Prod. 65, 556-564. Doi: 10.1016/j.indcrop.2014.09.060

Rashid, Z., M. Rashid, S. Inamullah, S. Rasool, and F.A. Bahar. 2013. Effect of different levels of farmyard manure and nitrogen on the yield and nitrogen uptake by stevia (Stevia rebaudiana Bertoni). Afr. J. Agric. Res. 8(29), 3941-3945.

SBCS, Sociedade Brasileira de Ciência do Solo. 2016. Manual de calagem e adubação para os estados do Rio Grande do Sul e Santa Catarina. 11th ed. Comissão de química e fertilidade do solo, Porto Alegre, Brazil.

Silva, F.A.S. and C.A.V. Azevedo. 2009. Principal components analysis in the software assistat-statistical attendance. Id. 711P0409e. In: Proc. 7th World Congress on Computers in Agriculture. Am. Soc. Agric. Biol. Eng., Reno, NV.

Sun, Y., M. Hou, L.A.J. Mur, Y. Yang, T. Zhang, X. Xu, S. Huang, and H. Tong. 2019. Nitrogen drives plant growth to the detriment of leaf sugar and steviol glycosides metabolisms in stevia (Stevia rebaudiana Bertoni). Plant Physiol. Biochem. 141, 240-249. Doi: 10.1016/j.plaphy.2019.06.008

Sun, Y., Y. Yang, M. Hou, X. Huang, T. Zhang, S. Huang, X. Xu, and H. Yuan. 2020. Optimized nitrogen topdressing strategies enhance steviol glycoside productivity in stevia (Stevia rebaudiana Bertoni) plants. J. Soil Sci. Plant Nutr. 20, 1133-1143. Doi: 10.1007/s42729-020-00199-w

Taiz, L. and E. Zeiger. 2013. Fisiologia vegetal. 5th ed. Artemed, Porto Alegre, Brazil.

Tavarini, S., C. Sgherri, A.M. Ranieri, and L.G. Angelini. 2015. Effect of nitrogen fertilization and harvest time on steviol glycosides, flavonoid composition and antioxidant properties in Stevia rebaudiana Bertoni. J. Agr. Food Chem. 63(31), 7041-7050. Doi: 10.1021/acs.jafc.5b02147

Teixeira, P.G., M.A.M. Carvalho, L.B.P. Zaidan, and A.L. Klein. 1997. Effect of mineral nutrients on growth and fructan contents in plants of Vernonia herbacea. Rev. Bras. Fisiol. Veg. 9(2), 89-96.

Yadav, A.K., S. Singh, D. Dhyani, and P.S. Ahuaja. 2011 A review on the improvement of stevia [Stevia rebaudiana Bertoni]. Can. J. Plant Sci. 91, 1-27. Doi: 10.4141/cjps10086

Yang, Y., S. Huang, Y. Han, H. Yuan, C. Gu, and Z. Wang. 2014. Environmental cues induce changes of steviol glycosides contents and transcription of corresponding biosynthetic genes in Stevia rebaudiana. Plant Physiol. Biochem. 86, 174-180. Doi: 10.1016/j.plaphy.2014.12.004

Yoneda, Y., H. Shimizu, H. Nakashima, J. Miyasaka, and K. Ohdoi. 2017. Effects of light intensity and photoperiod on improving steviol glycosides content in Stevia rebaudiana (Bertoni) Bertoni while conserving light energy consumption. J. Appl. Res. Med. Aroma. 7, 64-73. Doi: 10.1016/j.jarmap.2017.06.001

Watson, D.J. 1947. Comparative physiological studies on growth of field crops. I. Variation in net assimilation rate and leaf area between species and varieties, and within and between years. Ann. Bot. 11(1), 41-76. Doi: 10.1093/oxfordjournals.aob.a083148

Zaman, M.M., M.A.H. Chowdhury, and T. Chowdhury. 2015. Growth parameters and leaf biomass yield of stevia (Stevia rebaudiana Bertoni) as influenced by different soil types of Bangladesh. J. Bangladesh Agril. Univ. 13(1), 31-37.

Downloads

Download data is not yet available.

Most read articles by the same author(s)

Similar Articles

1 2 3 > >> 

You may also start an advanced similarity search for this article.