Phenotypic performance of four stevia genotypes in the Alto Vale do Itajaí region, Brazil
- Rômulo João Debarba
, - Cícero Deschamps,
- Luiz Alberto Kanis,
- Diego Moterle,
- Marcio Rampelotti
Abstract
An evaluation of four stevia genotypes for biomass yield, stevioside and rebaudioside A content and yield under decreasing photoperiod conditions was carried out in the Alto Vale do Itajaí region, located in the State of Santa Catarina (SC), Brazil. This field experiment was conducted at Site São Miguel, a farm located in the city of Lontras (SC), Brazil, under conditions of decreasing photoperiods, with a variation of 13.72 h of light at experiment implantation to 12.57 h of light at the end of the evaluations. The treatments consisted of four genotypes (G4, G8, G9 and G12) provided by EMBRAPA-CENARGEN. A randomized complete block design with four treatments (stevia genotypes) and four replications was used. Each plot consisted of 21 plants, and the floor area had five plants. G12 had the highest leaf dry weight (LDW), total leaf area, leaf area index, leaf area ratio and specific leaf area of all the genotypes. G4 and G12 were equal for LDW and were higher than the other genotypes, with yields of 755.6 and 836.4 kg ha-1, respectively. The stevioside content was highest in G12 (200.07 mg g-1). G8 and G9 were similar for rebaudioside A content (64.77 and 49.05 mg g-1, respectively). The rebaudioside A: stevioside ratio was highest in G8 (0.44 g g-1). No genotype had a rebaudioside A: stevioside ratio suitable for industry requirements.
Keywords
Photoperiodicity, Physiological response, Genotypes, Sweeteners, Stevia rebaudiana (Bert.) Bertoni, Stevioside, Rebaudioside
References
Anton, S.D., C.K. Martin, H. Han, S. Coulon, W.T. Cefalu, P. Geiselman, and D.A.Williamson. 2010. Effects of stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels. Appetite 55(1), 37-43. Doi: 10.1016/j.appet.2010.03.009
Bondarev, N.I., M.A. Sukhanova, G.A. Semenova, O.V. Goryaeva, S.E. Andreeva, and A.M. Nosov. 2010. Morphology and ultrastructure of trichomes of intact and in vitro plants of Stevia rebaudiana Bertoni with reference to biosynthesis and accumulation of steviol glycosides. Moscow Univ. Biol. Sci. Bull. 65(1), 12-16.
Carvalho, M.A.M. and L.B.P. Zaidan. 1995. Obtenção de plantas de Stevia rebaudiana através de estacas. Pesq. Agropec. Bras. 30(2), 201-206.
Ceunen, S. and J.M.C. Geuns. 2013. Spatio-temporal variation of the diterpene steviol in Stevia rebaudiana grown under different photoperiods. Phytochemistry 89, 32-38. Doi: 10.1016/j.phytochem.2013.01.007
Cunha, J.L.X.L., P.G.M.L. Nascimento, H.C. Mesquita, M.G.O. Silva, J.L.D. Dombroski, and I.N. Silva. 2010. Comparação de métodos de área foliar em Chrysobalanus icaco L. Agropecu. Cient. Semi-árido 6(3), 22-27.
Daza, M.C., J. Díaz, E. Aguirre, and N. Urrutia. 2015. Efecto de abonos de liberación lenta en la lixiviación de nitratos y nutrición nitrogenada en estevia. Rev. Colomb. Cienc. Hortic. 9(1), 112-123. Doi: 10.17584/rcch.2015v9i1.3750
Embrapa. 2006. Sistema brasileiro de classificação de solos. 2nd ed. Rio de Janeiro, Brazil.
Espita, M., R. Montoya, and L. Atencio. 2009. Rendimiento de Stevia rebaudiana Bert. bajo tres arreglos poblacionales en el Sinú Medio. Rev. UDCA Actual. Divulg. Cient. 12(1), 151-161.
Francisco, F. 2015. Acúmulo de biomassa, produção de esteviosídeo e rebaudiosídeo A e tolerância à geada e ao acamamento de acessos de Stevia rebaudiana Bert. MSc thesis. Pós-graduação em Agronomia, Universidade Federal do Parana, Brazil.
Hastoy, C., P. Cosson, S. Cavaignac, P. Boutié, P. Waffo-Teguo, D. Rolin, and V. Schurdi-Levraud. 2019. Deciphering performances of fifteen genotypes of Stevia rebaudiana in southwestern France through dry biomass and steviol glycoside evaluation. Ind. Crop. Prod. 128, 607-619. Doi: 10.1016/j.indcrop.2018.09.053
IFC, Instituto Federal Catarinense. 2015. Dados climáticos Campus Rio do Sul. Jardim América, Brazil.
Jarma, A., M. Espitia, M.E. Camacho, and G. Fischer. 2010. Síntesis de esteviosidos en estévia (Stevia rebaudiana Bert.). Acta Biol. Colomb. 15(1), 289-294.
Kolb, N., J.L. Herrera, D.J. Ferreyra, and R.F. Uliana. 2001. Analysis of sweet diterpene glycosides from Stevia rebaudiana: improved HPLC method. J. Agric. Food Chem. 49, 4538-4541. Doi: 10.1021/jf010475p
Kumar, R., S. Sharma, and S. Sood. 2014. Yield components, light interception and marker compound accumulation of stevia (Stevia rebaudiana Bertoni) affected by planting material and plant density under western Himalayan conditions. Arch. Agron. Soil Sci. 60(12), 1731-1745.
Lambers, H., F.S. Chapin III, and T.L. Pons. 2008. Plant physiological ecology. 2nd ed. Springer, New York, NY. Doi: 10.1007/978-0-387-78341-3
Lima Fiho, O.F., A.C.C. Valois, Z.M. Lucas, A.L. Melhorança, S. Maeda, C.R. Fietz, M.A. Urchei, A.M.A. Lícia, and F. Meneguetti. 2004. Estévia. Sistemas de Produção 5. Embrapa; Steviafarma Industrial, Maringá, Brazil.
Magalhães, A.C.N. 1979. Análise quantitativa do crescimento. pp. 331-350. In: Ferri, M.G. (ed.). Fisiologia vegetal. EPU/EDUSP, São Paulo, Brazil.
Mandal, S., H. Evelin, B. Giri, V.P. Singh, and R. Kapoor, 2013. Arbuscular mycorrhiza enhances the production of stevioside and rebaudioside-A in Stevia rebaudiana via nutritional and non-nutritional mechanisms. Appl. Soil Ecol. 72, 187-194. Doi: 10.1016/j.apsoil.2013.07.003
Mandal, S., S. Upadhyay, V.P. Singh, and R. Kapoor. 2015. Enhanced production of steviol glycosides in mycorrhizal plants: a concerted effect of arbuscular mycorrhizal symbiosis on transcription of biosynthetic genes. Plant Physiol. Biochem. 89, 100-106. Doi: 10.1016/j.plaphy.2015.02.010
MDIC, Ministério do Desenvolvimento, Indústria e Comércio Exterior. 2014. Sistema de Análisis de Información de Comercio Exterior (Aliceweb). In: http://aliceweb.desenvolvimento.gov.br; consulted: June, 2019.
Mota, T.R., A.S. Dacome, and S.C. Costa. 2011. Seleção de clones de elite de Stevia rebaudiana (Bert.) Bertoni com ênfase no teor do rebaudiosídeo A e a atividade antioxidante. Anais eletrônicos. In: VII EPCC – Encontro Internacional de Produção Científica Cesumar. CESUMAR; Centro Universitário de Maringá, Maringá, Brazil.
Munz, S., A. Präger, N. Merkt, W. Claupein, and S. Graeff-Hönninger. 2018. Leaf area index, light interception, growth and steviol glycoside formation of Stevia rebaudiana Bertoni under field conditions in southwestern Germany. Ind. Crop. Prod. 111, 520-528. Doi: 10.1016/j.indcrop.2017.11.021
Pal, P.K., M. Mahajan, R. Prasad, V. Pathania, B. Singh, and P.S. Ahuja. 2015. Harvesting regimes to optimize yield and quality in annual and perennial Stevia rebaudiana under sub-temperate conditions. Ind. Crops Prod. 65, 556-564. Doi: 10.1016/j.indcrop.2014.09.060
Parris, C.A., C.C. Shock, and M. Qian. 2016. Dry leaf and steviol glycoside productivity of Stevia rebaudiana in the Western United States. HortScience 51(10), 1220-1227. Doi: 10.21273/HORTSCI11149-16
Poorter, H. and E. Garnier. 1999. Ecological significance of inherent variation in relative growth rate and its components. pp. 81-120. In: Pugnaire, F.I. and F. Valladares (eds.). Handbook of functional plant. Marcel Dekker, New York, NY.
SBCS, Sociedade Brasileira de Ciência do Solo - Núcleo Regional Sul; CQFS, Comissão de Química e Fertilidade do Solo – RS/SC. 2016. Manual de calagem e adubação para os estados do Rio Grande do Sul e Santa Catarina. 11th ed. Viçosa, Brazil.
Serfaty, M., M. Ibdah, R. Fischer, D. Chaimovitsh, Y. Saranga, and N. Dudai. 2013. Dynamics of yield components and stevioside production in Stevia rebaudiana grown under different planting times, plant stands and harvesting regimes. Ind. Crops Prod. 50, 731-736. Doi: 10.1016/j.indcrop.2013.08.063
Silva, F.A.S. and C.A.V. Azevedo. 2009. Principal components analysis in the software assistat-statistical attendance. In: Proc. 7th World Congress on Computers in Agriculture. American Society of Agricultural and Biological Engineers, Reno, NV.
Tavarini, S., C. Sgherri, A.M. Ranieri, and L.G. Angelini. 2015. Effect of nitrogen fertilization and harvest time on steviol glycosides, flavonoid composition and antioxidant properties in Stevia rebaudiana Bertoni. J. Agr. Food Chem. 63(31), 7041-7050. Doi: 10.1021/acs.jafc.5b02147
Vasilakoglou, I., D. Kalfountzos, N. Gougoulias, and C. Reppas. 2016. Productivity of two stevia varieties under reduced irrigation and fertilization inputs. Arch. Agron. Soil Sci. 62(4), 457-472. Doi: 10.1080/03650340.2015.1060554
Watson, D.J. 1947. Comparative physiological studies on growth of field crops. I. Variation in net assimilation rate and leaf area between species and varieties, and within and between years. Ann. Bot. 11(1), 41-76. Doi: 10.1093/oxfordjournals.aob.a083148
Yoneda, Y., H. Shimizu, H. Nakashima, J. Miyasaka, and K. Ohdoi. 2017. Effects of light intensity and photoperiod on improving steviol glycosides content in Stevia rebaudiana (Bertoni) Bertoni while conserving light energy consumption. J. Appl. Res. Med. Aromat. Plants 7, 64-73. Doi: 10.1016/j.jarmap.2017.06.001