Genetic diversity of <i>Moringa oleifera</i> Lam. in the northeast of Colombia using RAMs markers
Article Sidebar
PDF (Español)
FLIP
Section: SECCIÓN DE HIERBAS AROMÁTICAS, MEDICINALES Y CONDIMENTARIAS
How to Cite
Chaves-Bedoya, G., Galvis-Perez, Z., & Ortiz-Rojas, L. Y. (2018). Genetic diversity of <i>Moringa oleifera</i> Lam. in the northeast of Colombia using RAMs markers. Revista Colombiana De Ciencias Hortícolas, 11(2), 408-415. https://doi.org/10.17584/rcch.2017v11i2.7343
More Citation Formats
Main Article Content
Autores
Giovanni Chaves-Bedoya
http://orcid.org/0000-0003-1013-614X
Zaida Lorena Galvis-Perez
Luz Yineth Ortiz-Rojas
http://orcid.org/0000-0002-0818-3089
Abstract
Moringa oleifera Lam., also known as Moringa pterygosperma Gaertn., is a fast growing tree, indigenous to Himalaya that has been used as food or as medicine, recognized for its bioactive compounds. In Colombia, moringa has been promoted primarily because of its medicinal properties. However, despite the importance of this phytogenetic resource, there are no studies on its genetic variability. In this study, we analyzed 45 accessions of moringa from four departments in the northeast of Colombia. The DICE and Nei-Li coefficients, at a level of similarity of 0.75, differentiated the population into four genetic groups. The expected heterozygosity was 0.13 for the oligo CT and 0.29 for the oligos CGA and GT. The percentage of polymorphic loci ranged from 31 to 100% for the primers CT and CA, respectively. With the UPGMA grouping, we revealed that the Moringa accessions from Villa del Rosario in Norte de Santander are genetically isolated. The genetic diversity of the studied Moringa accessions was low. This knowledge on the genetic variability of moringa provides a new contribution to Colombian growers that can be used in future breeding programs.
Article Details
Licence
The copyright of the articles and illustrations are the property of the Revista Colombiana de Ciencias Hortícolas. The editors authorize the use of the contents under the Creative Commons license Attribution-Noncommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). The correct citation of the content must explicitly register the name of the journal, name (s) of the author (s), year, title of the article, volume, number, page of the article and DOI. Written permission is required from publishers to publish more than a short summary of the text or figures.
References
Al-Asmari, A.K., S.M. Albalawi, M.T. Athar, A.Q. Khan, H. Al-Shahrani y M. Islam. 2015. Moringa oleifera as an anti-cancer agent against breast and colorectal cancer cell lines. PLoS One 10(8), e0135814. Doi: https://doi.org/10.1371/journal.pone.0135814
Aoki, K., S. Ueno, T. Kamijo, H. Setoguchi, N. Murakami, M. Kato y Y. Tsumura. 2014. Genetic differentiation and genetic diversity of Castanopsis (Fagaceae), the dominant tree species in Japanese broadleaved evergreen forests, revealed by analysis of EST-associated microsatellites. PLoS One 9, e87429. Doi: https://doi.org/10.1371/journal.pone.0087429
Chaves-Bedoya, G. y V. Nuñez. 2007. A SCAR marker for the sex types determination in Colombian genotypes of Carica papaya. Euphytica 153 (1-2), 215-220. Doi: https://doi.org/10.1007/s10681-006-9256-7
Cruz da Silva, A., A. ferreira dos Santos, A. Silva, R. Barroso, C. Almeida, G. Melo da Silva y M. Alves. 2012. Moringa genetic diversity from germoplasm bank using RAPD markers. Trop. Subtrop. Agroecosyst. 15, 31-39.
Engels, J.M.M., A.W. Ebert, I. Thormann y M.C. de Vicente. 2006. Centres of crop diversity and/or origin, genetically modified crops and implications for plant genetic resources conservation. Genet. Resour. Crop Evol. 53(8), 1675-1688. Doi: https://doi.org/10.1007/s10722-005-1215-y
Ganesan, S., R. Singh, D. Choudhuery, J. Bharadwaj, V. Gupta y A. Singode. 2014. Genetic diversity and population structure study of drumstick (Moringa oleifera Lam.) using morphological and SSR markers. Ind. Crops Prod. 60, 316-25. Doi: https://doi.org/10.1016/j.indcrop.2014.06.033
Jiang-Chong, W. e Y. Jing. 2010. Isolation and characterization of twenty polymorphic microsatellite loci for Moringa oleifera (Moringaceae). HortScience 45(4), 690-692.
Jungmann, L., B.B. Vigna, K.R. Boldrini, A.C. Sousa, C.B. do Valle, R.M. Resende, M.S. Pagliarini, M.I. Zucchi y A.P. de Souza. 2010. Genetic diversity and population structure analysis of the tropical pasture grass Brachiaria humidicola based on microsatellites, cytogenetics, morphological traits, and geographical origin. Genome 53(9), 698-709. Doi: https://doi.org/10.1139/G10-055
Kantartzi, S.K., M. Ulloa, E. Sacks y J.M. Stewart. 2009. Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites. Genet. 136(1), 141-147. Doi: https://doi.org/10.1007/s10709-008-9327-x
Krieg, J., D. Goetze, S. Porembski, P. Arnold, K.E. Linsenmair y K. Stein. 2017. Floral and reproductive biology of Moringa oleifera (Moringaceae) in Burkina Faso, West Africa. Acta Hortic. 1158, 63-69. Doi: https://doi.org/10.17660/ActaHortic.2017.1158.8
Kuhn, D.N., J.C. Motamayor, A.W. Meerow, J.W. Borrone y R.J. Schnell. 2008. SSCP markers provide a useful alternative to microsatellites in genotyping and estimating genetic diversity in populations and germplasm collections of plant specialty crops. Electrophoresis 29(19), 4096-4108. Doi: https://doi.org/10.1002/elps.200700937
Leone, A., A. Spada, A. Battezzati, A. Schiraldi, J. Aristil y S. Bertoli. 2015. Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. Int. J. Mol. Sci. 16(6), 12791-12835. Doi: https://doi.org/10.3390/ijms160612791
Liu, K., M. Goodman, S. Muse, J.S. Smith, E. Buckler y J. Doebley. 2003. Genetic structure and diversity among maize inbred lines as inferred from DNA microsatellites. Genet. 165(4), 2117-2128.
Martin, C., G. Martin, A. Garcia, T. Fernandez, E. Hernandez y J. Puls. 2013. Potenciales aplicaciones de Moringa oleifera. Una revisión crítica. Pastos Forrajes 36(2), 137-149.
Mbikay, M. 2012. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: A review. Front Pharmacol. 3, 24. Doi: https://doi.org/10.3389/fphar.2012.00024
Muller, F., M. Voccia, A.B. y J.M. Bouvet. 2009. Genetic diversity and gene flow in a Caribbean tree Pterocarpus officinalis Jacq.: a study based on chloroplast and nuclear microsatellites. Genet. 135(2), 185-198. Doi: https://doi.org/10.1007/s10709-008-9268-4
Muñoz, J., A. Morillo y Y. Morillo. 2008. Microsatelite amplificados al azar (RAM) en estudios de diversidad genética vegetal. Acta Agron. 57 (4), 219-226.
Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70(12), 3321-3323. Doi: https://doi.org/10.1073/pnas.70.12.3321
Nei, M. y W.H. Li. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76(2), 5269-5273. Doi: https://doi.org/10.1073/pnas.76.10.5269
Olson, M. 2002. Combining data from DNA sequences and morphology for a phylogeny of Moringaceae (Brassicales). Syst. Bot. 27(1), 55-73. Doi: https://doi.org/10.1043/0363-6445-27.1.55
Olson, M.E. y J.W. Fahey. 2011. Moringa oleifera: un árbol multiusos para las zonas tropicales secas. Rev. Mex. Biodiversidad 82, 1071-1082.
Rasouli, M., P. Martínez-Gómez y R. Karimi. 2015. Application of Random Amplified Microsatellite Polymorphism (RAMP) in Prunus characterization and mapping. J. Nuts 6(1), 1-5.
Rufai, S., M.M. Hanafi, M.Y. Rafii, S. Ahmad, I.W. Arolu y J. Ferdous. 2013. Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker. Biomed. Res. Int. 2013, 598-604. Doi: https://doi.org/10.1155/2013/604598
Saddoud, O., K. Chatti, A. Salhi-Hannachi, M. Mars, A. Rhouma, M. Marrakchi y M. Trifi. 2007. Genetic diversity of Tunisian figs (Ficus carica L.) as revealed by nuclear microsatellites. Hereditas 144(4), 149-157. Doi: https://doi.org/10.1111/j.2007.0018-0661.01967.x
Saini, R.K., K.R. Saad, G.A. Ravishankar, P. Giridhar y N.P. Shetty 2013. Genetic diversity of commercially grown Moringa oleifera Lam. cultivars from India by RAPD, SSR and cytochrome P450-based markers. Plant Syst. Evol. 299(7), 1205-1213. Doi: https://doi.org/10.1007/s00606-013-0789-7
Sanabria O., H., M. García, J. Muñoz y H. Diaz. 2006. Caracterización molecular con marcadores RAM de árboles nativos de Psidium guajava (guayaba) en el Valle del Cauca. Acta Agron. 55(1), 23-30.
Shahzad, U., M. Awais, M. Jaffar, I. Ahmad y S. Korban. 2013. Genetic diversity and population structure of Moringa oleifera. Conserv. Genet. 14(6), 1161-1172. Doi: https://doi.org/10.1007/s10592-013-0503-x
Shamsuddeen, R., M. Hanafi, M. Rafii, S. Ahmad, I. Arolu y J. Ferdous. 2013. Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using Random Amplified Polymorphic DNA Marker. BioMed Res. Int. 2013, 604598. Doi: https://doi.org/10.1155/2013/604598
Sun, Y., X. Wen y H. Huang. 2011. Genetic diversity and population structure of two important medicinal plant species Schisandra chinensis and Schisandra sphenanthera revealed by nuclear microsatellites. Genet. 139(4), 497-503. Doi: https://doi.org/10.1007/s10709-011-9570-4
Wu, J. y J. Yang. 2010. Isolation and characterization of twenty polymorphic microsatellite loci for Moringa oleifera (Moringaceae). HortScience 45(4), 690-692.
Aoki, K., S. Ueno, T. Kamijo, H. Setoguchi, N. Murakami, M. Kato y Y. Tsumura. 2014. Genetic differentiation and genetic diversity of Castanopsis (Fagaceae), the dominant tree species in Japanese broadleaved evergreen forests, revealed by analysis of EST-associated microsatellites. PLoS One 9, e87429. Doi: https://doi.org/10.1371/journal.pone.0087429
Chaves-Bedoya, G. y V. Nuñez. 2007. A SCAR marker for the sex types determination in Colombian genotypes of Carica papaya. Euphytica 153 (1-2), 215-220. Doi: https://doi.org/10.1007/s10681-006-9256-7
Cruz da Silva, A., A. ferreira dos Santos, A. Silva, R. Barroso, C. Almeida, G. Melo da Silva y M. Alves. 2012. Moringa genetic diversity from germoplasm bank using RAPD markers. Trop. Subtrop. Agroecosyst. 15, 31-39.
Engels, J.M.M., A.W. Ebert, I. Thormann y M.C. de Vicente. 2006. Centres of crop diversity and/or origin, genetically modified crops and implications for plant genetic resources conservation. Genet. Resour. Crop Evol. 53(8), 1675-1688. Doi: https://doi.org/10.1007/s10722-005-1215-y
Ganesan, S., R. Singh, D. Choudhuery, J. Bharadwaj, V. Gupta y A. Singode. 2014. Genetic diversity and population structure study of drumstick (Moringa oleifera Lam.) using morphological and SSR markers. Ind. Crops Prod. 60, 316-25. Doi: https://doi.org/10.1016/j.indcrop.2014.06.033
Jiang-Chong, W. e Y. Jing. 2010. Isolation and characterization of twenty polymorphic microsatellite loci for Moringa oleifera (Moringaceae). HortScience 45(4), 690-692.
Jungmann, L., B.B. Vigna, K.R. Boldrini, A.C. Sousa, C.B. do Valle, R.M. Resende, M.S. Pagliarini, M.I. Zucchi y A.P. de Souza. 2010. Genetic diversity and population structure analysis of the tropical pasture grass Brachiaria humidicola based on microsatellites, cytogenetics, morphological traits, and geographical origin. Genome 53(9), 698-709. Doi: https://doi.org/10.1139/G10-055
Kantartzi, S.K., M. Ulloa, E. Sacks y J.M. Stewart. 2009. Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites. Genet. 136(1), 141-147. Doi: https://doi.org/10.1007/s10709-008-9327-x
Krieg, J., D. Goetze, S. Porembski, P. Arnold, K.E. Linsenmair y K. Stein. 2017. Floral and reproductive biology of Moringa oleifera (Moringaceae) in Burkina Faso, West Africa. Acta Hortic. 1158, 63-69. Doi: https://doi.org/10.17660/ActaHortic.2017.1158.8
Kuhn, D.N., J.C. Motamayor, A.W. Meerow, J.W. Borrone y R.J. Schnell. 2008. SSCP markers provide a useful alternative to microsatellites in genotyping and estimating genetic diversity in populations and germplasm collections of plant specialty crops. Electrophoresis 29(19), 4096-4108. Doi: https://doi.org/10.1002/elps.200700937
Leone, A., A. Spada, A. Battezzati, A. Schiraldi, J. Aristil y S. Bertoli. 2015. Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. Int. J. Mol. Sci. 16(6), 12791-12835. Doi: https://doi.org/10.3390/ijms160612791
Liu, K., M. Goodman, S. Muse, J.S. Smith, E. Buckler y J. Doebley. 2003. Genetic structure and diversity among maize inbred lines as inferred from DNA microsatellites. Genet. 165(4), 2117-2128.
Martin, C., G. Martin, A. Garcia, T. Fernandez, E. Hernandez y J. Puls. 2013. Potenciales aplicaciones de Moringa oleifera. Una revisión crítica. Pastos Forrajes 36(2), 137-149.
Mbikay, M. 2012. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: A review. Front Pharmacol. 3, 24. Doi: https://doi.org/10.3389/fphar.2012.00024
Muller, F., M. Voccia, A.B. y J.M. Bouvet. 2009. Genetic diversity and gene flow in a Caribbean tree Pterocarpus officinalis Jacq.: a study based on chloroplast and nuclear microsatellites. Genet. 135(2), 185-198. Doi: https://doi.org/10.1007/s10709-008-9268-4
Muñoz, J., A. Morillo y Y. Morillo. 2008. Microsatelite amplificados al azar (RAM) en estudios de diversidad genética vegetal. Acta Agron. 57 (4), 219-226.
Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70(12), 3321-3323. Doi: https://doi.org/10.1073/pnas.70.12.3321
Nei, M. y W.H. Li. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76(2), 5269-5273. Doi: https://doi.org/10.1073/pnas.76.10.5269
Olson, M. 2002. Combining data from DNA sequences and morphology for a phylogeny of Moringaceae (Brassicales). Syst. Bot. 27(1), 55-73. Doi: https://doi.org/10.1043/0363-6445-27.1.55
Olson, M.E. y J.W. Fahey. 2011. Moringa oleifera: un árbol multiusos para las zonas tropicales secas. Rev. Mex. Biodiversidad 82, 1071-1082.
Rasouli, M., P. Martínez-Gómez y R. Karimi. 2015. Application of Random Amplified Microsatellite Polymorphism (RAMP) in Prunus characterization and mapping. J. Nuts 6(1), 1-5.
Rufai, S., M.M. Hanafi, M.Y. Rafii, S. Ahmad, I.W. Arolu y J. Ferdous. 2013. Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker. Biomed. Res. Int. 2013, 598-604. Doi: https://doi.org/10.1155/2013/604598
Saddoud, O., K. Chatti, A. Salhi-Hannachi, M. Mars, A. Rhouma, M. Marrakchi y M. Trifi. 2007. Genetic diversity of Tunisian figs (Ficus carica L.) as revealed by nuclear microsatellites. Hereditas 144(4), 149-157. Doi: https://doi.org/10.1111/j.2007.0018-0661.01967.x
Saini, R.K., K.R. Saad, G.A. Ravishankar, P. Giridhar y N.P. Shetty 2013. Genetic diversity of commercially grown Moringa oleifera Lam. cultivars from India by RAPD, SSR and cytochrome P450-based markers. Plant Syst. Evol. 299(7), 1205-1213. Doi: https://doi.org/10.1007/s00606-013-0789-7
Sanabria O., H., M. García, J. Muñoz y H. Diaz. 2006. Caracterización molecular con marcadores RAM de árboles nativos de Psidium guajava (guayaba) en el Valle del Cauca. Acta Agron. 55(1), 23-30.
Shahzad, U., M. Awais, M. Jaffar, I. Ahmad y S. Korban. 2013. Genetic diversity and population structure of Moringa oleifera. Conserv. Genet. 14(6), 1161-1172. Doi: https://doi.org/10.1007/s10592-013-0503-x
Shamsuddeen, R., M. Hanafi, M. Rafii, S. Ahmad, I. Arolu y J. Ferdous. 2013. Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using Random Amplified Polymorphic DNA Marker. BioMed Res. Int. 2013, 604598. Doi: https://doi.org/10.1155/2013/604598
Sun, Y., X. Wen y H. Huang. 2011. Genetic diversity and population structure of two important medicinal plant species Schisandra chinensis and Schisandra sphenanthera revealed by nuclear microsatellites. Genet. 139(4), 497-503. Doi: https://doi.org/10.1007/s10709-011-9570-4
Wu, J. y J. Yang. 2010. Isolation and characterization of twenty polymorphic microsatellite loci for Moringa oleifera (Moringaceae). HortScience 45(4), 690-692.
Downloads
Download data is not yet available.
