Potential use of electrochemically synthesized silver nanoparticles on Rice Panicle Blight Pathogen Burkholderia glumae

Uso potencial de nanopartículas de plata sintetizadas electroquímicamente sobre el patógeno Burkholderia glumae causante del tizón de la panícula del arroz

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Giovanni Chaves-Bedoya
Hilda Angélica Padilla
Luz Yineth Ortiz-Rojas
Gabriel Peña-Rodríguez

Abstract

Burkholderia glumae is the main causal agent of Bacterial Panicle Blight (BPB) in rice (Oriza sativa L.), a disease that generates losses in production worldwide. Despite its economic importance, effective control measures and varieties of rice with complete resistance to the disease have not yet been available. In this study, the antimicrobial activity of electrochemically synthesized silver nanoparticles (AgNPs) against B. glumae was evaluated. For the AgNPs synthesis, a DC power supply (UNI-T®) regulated at 24 V was used, which was connected to two cylindrical electrodes of high purity silver (Aldrich-99.99%), using distilled water as electrolyte. The concentration of AgNPs was determined by measuring the total dissolved solids (TDS) through the multiparameter HandyLab 680 FK. The antibacterial activity of these nanoparticles against B. glumae was determined by the method of broth macrodilution method, at different concentrations (1-10 mg L-1). The Minimum Inhibitory Concentration (MIC) was determined in 5 mg L-1 of AgNPs. The results of this study revealed that AgNPs could be considered as promising nanopesticide to control BPB disease in rice.

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