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Vol. 16 No. 2 (2022)

Fruits section

Correlation between SPAD and chlorophylls a, b and total in leaves from Vaccinium corymbosum L. cv. Biloxi, Legacy and Victoria in the high tropics

https://doi.org/10.17584/rcch.2022v16i2.14693

Published 2022-05-01

  • Elberth Hernando Pinzón-Sandoval
    • ,
  • Pedro José Almanza-Merchán
    • ,
  • Germán Eduardo Cely-Reyes
    • ,
  • Pablo Antonio Serrano-Cely
    • ,
  • Guillermo Alexander Ayala-Martínez

Elberth Hernando Pinzón-Sandoval
Universidad Pedagógica y Tecnológica de Colombia, Facultad de Ciencias Agropecuarias, Grupo de Investigación en Desarrollo y Producción Agraria Sostenible, Tunja

Pedro José Almanza-Merchán
Universidad Pedagógica y Tecnológica de Colombia, Facultad de Ciencias Agropecuarias, Grupo de Investigación en Desarrollo y Producción Agraria Sostenible, Tunja

Germán Eduardo Cely-Reyes
Universidad Pedagógica y Tecnológica de Colombia, Facultad de Ciencias Agropecuarias, Grupo de Investigación en Desarrollo y Producción Agraria Sostenible, Tunja

Pablo Antonio Serrano-Cely
Universidad Pedagógica y Tecnológica de Colombia, Facultad de Ciencias Agropecuarias, Grupo de Investigación en Desarrollo y Producción Agraria Sostenible, Tunja

Guillermo Alexander Ayala-Martínez
Universidad Pedagógica y Tecnológica de Colombia, Facultad de Ciencias Agropecuarias, Grupo de Investigación en Desarrollo y Producción Agraria Sostenible, Tunja

Blueberry cv. Biloxi. Photo: E.H. Pinzón-Sandoval

Abstract

Blueberries in the Ericaceae family are important, cultivable species for commercial and industrial purposes. Critical parameters for evaluating the nutritional and physiological status of plants include estimates of photosynthetic pigments such as chlorophylls; however, this is a destructive, expensive and time-consuming method that is not easily accessible for agricultural producers. For this reason, in recent years, several technological solutions have been made available, such as the SPAD-502 chlorophyll index meter, which has demonstrated efficiency in terms of rapid estimates of total chlorophyll content in the field. Therefore, the objective of this research was to evaluate the contents of chlorophylls a, b and total obtained with spectrophotometry and to determine the correlation with the non-destructive method (SPAD) in blueberry plant (Vaccinium corymbosum L.) cultivares Biloxi, Legacy and Victoria, in the vegetative phase under conditions of the municipality of Paipa-Boyaca (Colombia). The data obtained with the SPAD-502 ranged from 57.4 to 62.8 SPAD in the 'Victoria', 61.2 to 68.3 in the 'Biloxi', and 68.2 to 73.1 in the 'Legacy'. The content of chlorophylls a, b and total presented a positive and significant correlation with the SPAD index, with a correlation coefficient (r) greater than 0.91 and adjustment to simple linear regression models with a coefficient of determination (R2) greater than 0.90 in the three cultivars. This confirms the usefulness and importance of using SPAD for non-destructive estimation of chlorophyll content under field conditions.

Keywords

Blueberry, Cultivars, Photosynthetic pigments, Physiological status

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