Morphometry, viability and germination of seeds of Benincasa hispida [(Thunb.) Cogn.]
- Miguel Esptia-Camacho
, - Alisson Villalba-Soto,
- Hermes Aramédiz-Tatis,
- Carlos Cardona-Ayala
Abstract
The objective of the study was to evaluate the morphometry, viability and germination of B. hispida seeds, at the Universidad de Córdoba, Colombia. Eight biometric characteristics were measured in a sample of seeds taken at random from a balanced compound originating from 50 fruits. The external and internal morphological description was made on a sample of 10 seeds. Seed viability was estimated by the tetrazolium test, using the combination of two staining times (2 and 3 hours) and three concentrations of (0.5, 1.0 and 1.5%), under a design completely randomized, with six treatments and four repetitions of 50 seeds. The germination test (PRUGER) was performed in a mesh house with four repetitions of 50 seeds to estimate eight physiological parameters of germination. The morpho-metric characteristics of thickness, length and width of seeds showed reduced phenotypic variation, compared with the weight, density and volume of a seed, with variation coefficients greater than 11.7%. The seed, ovoid and flattened, presents an embryo with its cotyledons wrapped in a protective membrane. The viability, determined with tetrazolium, was 89.5-96.5%, while the PRUGER was 100%, with no statistical differences between them (P≥0.05). The combination of 0.5% tetrazolium with 2 hours is sufficient to determine the viability of the seed. Germination elapsed from 6 to 19 days after sowing, with a germination speed index of 0.22, average daily germination of 5.26%, peak value of 5.33 and germination value of 28.
Keywords
Winter gourd, Biometric characteristics, Tetrazolium test, Physiological quality
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