Didactic intervention for the extraction and quantification of steroids in urine
- Diego Hernando Angulo
, - Jaime Augusto Casas,
- Edna Carolina Cipagauta,
- David Eduardo Aparicio
Abstract
This article describes a didactic strategy for teaching a miniaturized solid-phase extraction
method in the context of laboratory practical training [LPT]. It is based on the students’
previous imaginaries in order to generate conceptual changes and promote meaningful learning through a few laboratory sessions. This article will report on the experience of combining the use of a sample preparation technique—PT-SPE—with a didactic intervention exercise for the improvement of procedural skills. We present the results of the application of LPT in the multi-determination of three steroids in urine samples using HPLC-DAD-PT-C18-SPE, which show adequate recovery rates and validation parameters. The results of the intervention show improvement of skills related to solid-phase extraction methods. It is concluded that there were conceptual changes in analytical processes, an adequate familiarization with new sample preparation methodologies, a significant increase in chemical vocabulary,and understanding of concepts, along with the application of lower-order cognitive processes to the enhancement of higher-order cognitive skills.
Keywords
experimental science didactics, laboratory practical training, sample preparation, SPE, HPLC
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